Iron in PDB 6yf4: [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Enzymatic activity of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
All present enzymatic activity of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D:
1.12.7.2;
Protein crystallography data
The structure of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D, PDB code: 6yf4
was solved by
J.Duan,
E.Hofmann,
T.Happe,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
47.48 /
1.77
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
89.810,
72.020,
102.930,
90.00,
97.48,
90.00
|
R / Rfree (%)
|
20 /
23.4
|
Other elements in 6yf4:
The structure of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D also contains other interesting chemical elements:
Iron Binding Sites:
Iron binding site 1 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 1 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe601
b:26.4
occ:1.00
|
FE1
|
A:402601
|
0.0
|
26.4
|
1.0
|
C3
|
A:402601
|
1.7
|
19.5
|
1.0
|
C4
|
A:402601
|
1.8
|
26.9
|
1.0
|
C5
|
A:402601
|
1.9
|
26.8
|
1.0
|
S1
|
A:402601
|
2.3
|
31.1
|
1.0
|
S2
|
A:402601
|
2.3
|
25.8
|
1.0
|
SG
|
A:CYS503
|
2.3
|
25.8
|
1.0
|
FE2
|
A:402601
|
2.6
|
31.5
|
1.0
|
O3
|
A:402601
|
3.0
|
32.6
|
1.0
|
N4
|
A:402601
|
3.0
|
28.4
|
1.0
|
O5
|
A:402601
|
3.1
|
29.6
|
1.0
|
HB3
|
A:CYS503
|
3.2
|
26.8
|
1.0
|
CB
|
A:CYS503
|
3.2
|
22.4
|
1.0
|
HB2
|
A:CYS503
|
3.4
|
26.8
|
1.0
|
C1
|
A:402601
|
3.4
|
27.7
|
1.0
|
C2
|
A:402601
|
3.5
|
30.5
|
1.0
|
C7
|
A:402601
|
3.8
|
39.8
|
1.0
|
C6
|
A:402601
|
3.8
|
34.4
|
1.0
|
HD2
|
A:PRO231
|
3.9
|
27.8
|
1.0
|
FE4
|
A:SF4602
|
4.0
|
22.7
|
1.0
|
N1
|
A:402601
|
4.0
|
29.1
|
1.0
|
HE3
|
A:LYS358
|
4.0
|
30.9
|
1.0
|
HB1
|
A:ALA230
|
4.1
|
29.5
|
1.0
|
S2
|
A:SF4602
|
4.3
|
22.3
|
1.0
|
HA2
|
A:GLY418
|
4.3
|
34.4
|
1.0
|
HD2
|
A:PRO354
|
4.3
|
32.6
|
1.0
|
HG2
|
A:PRO354
|
4.4
|
29.7
|
1.0
|
HG3
|
A:PRO354
|
4.6
|
29.7
|
1.0
|
HE2
|
A:LYS358
|
4.6
|
30.9
|
1.0
|
CA
|
A:CYS503
|
4.7
|
26.4
|
1.0
|
HG2
|
A:PRO231
|
4.7
|
30.4
|
1.0
|
N6
|
A:402601
|
4.7
|
20.6
|
1.0
|
CE
|
A:LYS358
|
4.8
|
25.7
|
1.0
|
CG
|
A:PRO354
|
4.8
|
24.8
|
1.0
|
CD
|
A:PRO231
|
4.8
|
23.2
|
1.0
|
O7
|
A:402601
|
4.8
|
35.0
|
1.0
|
HA
|
A:CYS503
|
4.9
|
31.7
|
1.0
|
CB
|
A:ALA230
|
4.9
|
24.6
|
1.0
|
HB2
|
A:ALA230
|
4.9
|
29.5
|
1.0
|
CD
|
A:PRO354
|
4.9
|
27.1
|
1.0
|
H
|
A:CYS503
|
5.0
|
29.0
|
1.0
|
HG
|
A:SER232
|
5.0
|
33.4
|
1.0
|
|
Iron binding site 2 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 2 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe601
b:31.5
occ:1.00
|
FE2
|
A:402601
|
0.0
|
31.5
|
1.0
|
C7
|
A:402601
|
1.8
|
39.8
|
1.0
|
C6
|
A:402601
|
1.9
|
34.4
|
1.0
|
C5
|
A:402601
|
2.0
|
26.8
|
1.0
|
S2
|
A:402601
|
2.3
|
25.8
|
1.0
|
S1
|
A:402601
|
2.3
|
31.1
|
1.0
|
FE1
|
A:402601
|
2.6
|
26.4
|
1.0
|
O5
|
A:402601
|
2.8
|
29.6
|
1.0
|
O7
|
A:402601
|
2.9
|
35.0
|
1.0
|
N6
|
A:402601
|
3.1
|
20.6
|
1.0
|
HG2
|
A:PRO324
|
3.1
|
32.9
|
1.0
|
N1
|
A:402601
|
3.3
|
29.1
|
1.0
|
C1
|
A:402601
|
3.4
|
27.7
|
1.0
|
C2
|
A:402601
|
3.4
|
30.5
|
1.0
|
C4
|
A:402601
|
3.6
|
26.9
|
1.0
|
HD2
|
A:PRO324
|
3.7
|
35.5
|
1.0
|
C3
|
A:402601
|
3.7
|
19.5
|
1.0
|
HB2
|
A:PRO324
|
3.7
|
31.6
|
1.0
|
HD2
|
A:PHE417
|
3.8
|
30.7
|
1.0
|
HE3
|
A:LYS358
|
3.9
|
30.9
|
1.0
|
CG
|
A:PRO324
|
3.9
|
27.4
|
1.0
|
HD2
|
A:PRO231
|
3.9
|
27.8
|
1.0
|
HB3
|
A:PHE417
|
4.1
|
33.9
|
1.0
|
HZ3
|
A:LYS358
|
4.3
|
27.1
|
1.0
|
CD
|
A:PRO324
|
4.3
|
29.6
|
1.0
|
CB
|
A:PRO324
|
4.3
|
26.3
|
1.0
|
HG2
|
A:PRO231
|
4.3
|
30.4
|
1.0
|
HB2
|
A:CYS299
|
4.3
|
39.7
|
1.0
|
SG
|
A:CYS503
|
4.5
|
25.8
|
1.0
|
HD3
|
A:PRO231
|
4.5
|
27.8
|
1.0
|
CD
|
A:PRO231
|
4.6
|
23.2
|
1.0
|
HG21
|
A:ILE268
|
4.6
|
32.0
|
1.0
|
HG22
|
A:ILE268
|
4.6
|
32.0
|
1.0
|
N4
|
A:402601
|
4.6
|
28.4
|
1.0
|
HZ1
|
A:LYS358
|
4.6
|
27.1
|
1.0
|
CD2
|
A:PHE417
|
4.7
|
25.6
|
1.0
|
CE
|
A:LYS358
|
4.7
|
25.7
|
1.0
|
HG3
|
A:PRO324
|
4.7
|
32.9
|
1.0
|
NZ
|
A:LYS358
|
4.7
|
22.5
|
1.0
|
HB1
|
A:ALA230
|
4.8
|
29.5
|
1.0
|
O3
|
A:402601
|
4.8
|
32.6
|
1.0
|
HB3
|
A:PRO324
|
4.8
|
31.6
|
1.0
|
HD3
|
A:PRO324
|
4.9
|
35.5
|
1.0
|
HE2
|
A:LYS358
|
4.9
|
30.9
|
1.0
|
CG
|
A:PRO231
|
4.9
|
25.4
|
1.0
|
H
|
A:GLN325
|
4.9
|
26.9
|
1.0
|
CB
|
A:PHE417
|
5.0
|
28.3
|
1.0
|
|
Iron binding site 3 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 3 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:23.6
occ:1.00
|
FE1
|
A:SF4602
|
0.0
|
23.6
|
1.0
|
SG
|
A:CYS355
|
2.3
|
29.7
|
1.0
|
S2
|
A:SF4602
|
2.3
|
22.3
|
1.0
|
S3
|
A:SF4602
|
2.3
|
20.3
|
1.0
|
S4
|
A:SF4602
|
2.3
|
22.1
|
1.0
|
HB2
|
A:CYS355
|
2.7
|
37.0
|
1.0
|
FE4
|
A:SF4602
|
2.7
|
22.7
|
1.0
|
FE3
|
A:SF4602
|
2.7
|
24.4
|
1.0
|
FE2
|
A:SF4602
|
2.7
|
21.7
|
1.0
|
CB
|
A:CYS355
|
3.0
|
30.8
|
1.0
|
HB3
|
A:CYS355
|
3.6
|
37.0
|
1.0
|
O
|
A:HOH751
|
3.7
|
21.1
|
1.0
|
HB3
|
A:SER357
|
3.8
|
36.9
|
1.0
|
HD2
|
A:PRO301
|
3.9
|
35.3
|
1.0
|
S1
|
A:SF4602
|
3.9
|
20.6
|
1.0
|
O3
|
A:402601
|
4.0
|
32.6
|
1.0
|
HG2
|
A:LYS358
|
4.0
|
29.1
|
1.0
|
HG2
|
A:PRO301
|
4.1
|
33.7
|
1.0
|
HA
|
A:CYS355
|
4.1
|
29.0
|
1.0
|
HE2
|
A:LYS358
|
4.2
|
30.9
|
1.0
|
CA
|
A:CYS355
|
4.2
|
24.2
|
1.0
|
HB2
|
A:SER357
|
4.3
|
36.9
|
1.0
|
H
|
A:SER357
|
4.3
|
29.5
|
1.0
|
H
|
A:GLY302
|
4.4
|
26.8
|
1.0
|
CB
|
A:SER357
|
4.5
|
30.7
|
1.0
|
C3
|
A:402601
|
4.6
|
19.5
|
1.0
|
H
|
A:LYS358
|
4.6
|
30.1
|
1.0
|
CD
|
A:PRO301
|
4.7
|
29.4
|
1.0
|
SG
|
A:CYS503
|
4.7
|
25.8
|
1.0
|
SG
|
A:CYS499
|
4.7
|
22.8
|
1.0
|
SG
|
A:CYS300
|
4.8
|
22.9
|
1.0
|
CG
|
A:PRO301
|
4.8
|
28.1
|
1.0
|
CG
|
A:LYS358
|
4.9
|
24.2
|
1.0
|
HA
|
A:CYS300
|
4.9
|
30.5
|
1.0
|
HA
|
A:CYS503
|
4.9
|
31.7
|
1.0
|
H
|
A:GLY507
|
5.0
|
25.5
|
1.0
|
|
Iron binding site 4 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 4 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:21.7
occ:1.00
|
FE2
|
A:SF4602
|
0.0
|
21.7
|
1.0
|
SG
|
A:CYS499
|
2.3
|
22.8
|
1.0
|
S4
|
A:SF4602
|
2.3
|
22.1
|
1.0
|
S1
|
A:SF4602
|
2.3
|
20.6
|
1.0
|
S3
|
A:SF4602
|
2.3
|
20.3
|
1.0
|
FE3
|
A:SF4602
|
2.7
|
24.4
|
1.0
|
FE1
|
A:SF4602
|
2.7
|
23.6
|
1.0
|
FE4
|
A:SF4602
|
2.7
|
22.7
|
1.0
|
HB2
|
A:CYS499
|
2.9
|
19.1
|
1.0
|
CB
|
A:CYS499
|
3.2
|
15.9
|
1.0
|
H
|
A:CYS499
|
3.4
|
31.1
|
1.0
|
HA2
|
A:GLY506
|
3.6
|
25.6
|
1.0
|
HB3
|
A:ALA498
|
3.8
|
30.3
|
1.0
|
HA
|
A:CYS503
|
3.8
|
31.7
|
1.0
|
N
|
A:CYS499
|
3.8
|
25.9
|
1.0
|
HB3
|
A:CYS499
|
3.9
|
19.1
|
1.0
|
S2
|
A:SF4602
|
3.9
|
22.3
|
1.0
|
O
|
A:HOH751
|
4.1
|
21.1
|
1.0
|
CA
|
A:CYS499
|
4.1
|
20.8
|
1.0
|
H
|
A:ALA498
|
4.4
|
25.1
|
1.0
|
H
|
A:GLY507
|
4.4
|
25.5
|
1.0
|
CA
|
A:GLY506
|
4.4
|
21.4
|
1.0
|
HA3
|
A:GLY506
|
4.5
|
25.6
|
1.0
|
HB2
|
A:CYS193
|
4.5
|
25.8
|
1.0
|
HB2
|
A:CYS355
|
4.5
|
37.0
|
1.0
|
SG
|
A:CYS355
|
4.6
|
29.7
|
1.0
|
HA
|
A:CYS499
|
4.7
|
25.0
|
1.0
|
CB
|
A:ALA498
|
4.7
|
25.3
|
1.0
|
C
|
A:ALA498
|
4.7
|
29.4
|
1.0
|
CA
|
A:CYS503
|
4.7
|
26.4
|
1.0
|
SG
|
A:CYS300
|
4.8
|
22.9
|
1.0
|
N
|
A:ALA498
|
4.9
|
20.9
|
1.0
|
O
|
A:GLY502
|
4.9
|
26.8
|
1.0
|
HE21
|
A:GLN195
|
4.9
|
29.7
|
1.0
|
SG
|
A:CYS503
|
4.9
|
25.8
|
1.0
|
CA
|
A:ALA498
|
5.0
|
22.6
|
1.0
|
|
Iron binding site 5 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 5 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:24.4
occ:1.00
|
FE3
|
A:SF4602
|
0.0
|
24.4
|
1.0
|
SG
|
A:CYS300
|
2.3
|
22.9
|
1.0
|
S4
|
A:SF4602
|
2.3
|
22.1
|
1.0
|
S1
|
A:SF4602
|
2.3
|
20.6
|
1.0
|
S2
|
A:SF4602
|
2.3
|
22.3
|
1.0
|
FE2
|
A:SF4602
|
2.7
|
21.7
|
1.0
|
FE1
|
A:SF4602
|
2.7
|
23.6
|
1.0
|
FE4
|
A:SF4602
|
2.7
|
22.7
|
1.0
|
H
|
A:ALA498
|
3.2
|
25.1
|
1.0
|
HB2
|
A:CYS300
|
3.3
|
28.9
|
1.0
|
HB3
|
A:ALA498
|
3.3
|
30.3
|
1.0
|
CB
|
A:CYS300
|
3.3
|
24.1
|
1.0
|
HA
|
A:CYS300
|
3.5
|
30.5
|
1.0
|
H
|
A:GLY302
|
3.6
|
26.8
|
1.0
|
HD2
|
A:PRO301
|
3.8
|
35.3
|
1.0
|
S3
|
A:SF4602
|
3.9
|
20.3
|
1.0
|
CA
|
A:CYS300
|
3.9
|
25.4
|
1.0
|
N
|
A:ALA498
|
4.0
|
20.9
|
1.0
|
H
|
A:CYS499
|
4.0
|
31.1
|
1.0
|
CB
|
A:ALA498
|
4.1
|
25.3
|
1.0
|
HB3
|
A:CYS300
|
4.2
|
28.9
|
1.0
|
HB2
|
A:ALA498
|
4.3
|
30.3
|
1.0
|
HA3
|
A:GLY302
|
4.3
|
28.2
|
1.0
|
HA
|
A:MET497
|
4.3
|
37.9
|
1.0
|
C
|
A:CYS300
|
4.4
|
26.1
|
1.0
|
N
|
A:GLY302
|
4.4
|
22.4
|
1.0
|
H
|
A:TRP303
|
4.4
|
31.9
|
1.0
|
CD
|
A:PRO301
|
4.6
|
29.4
|
1.0
|
N
|
A:PRO301
|
4.6
|
23.4
|
1.0
|
HB3
|
A:MET497
|
4.6
|
32.5
|
1.0
|
CA
|
A:ALA498
|
4.6
|
22.6
|
1.0
|
SG
|
A:CYS499
|
4.7
|
22.8
|
1.0
|
N
|
A:CYS499
|
4.7
|
25.9
|
1.0
|
SG
|
A:CYS503
|
4.7
|
25.8
|
1.0
|
SD
|
A:MET497
|
4.8
|
30.5
|
1.0
|
HG2
|
A:PRO301
|
4.8
|
33.7
|
1.0
|
SG
|
A:CYS355
|
4.9
|
29.7
|
1.0
|
CA
|
A:GLY302
|
4.9
|
23.5
|
1.0
|
HB2
|
A:CYS499
|
4.9
|
19.1
|
1.0
|
HB1
|
A:ALA498
|
4.9
|
30.3
|
1.0
|
C
|
A:MET497
|
5.0
|
25.1
|
1.0
|
CA
|
A:MET497
|
5.0
|
31.6
|
1.0
|
|
Iron binding site 6 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 6 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:22.7
occ:1.00
|
FE4
|
A:SF4602
|
0.0
|
22.7
|
1.0
|
SG
|
A:CYS503
|
2.3
|
25.8
|
1.0
|
S3
|
A:SF4602
|
2.3
|
20.3
|
1.0
|
S2
|
A:SF4602
|
2.3
|
22.3
|
1.0
|
S1
|
A:SF4602
|
2.3
|
20.6
|
1.0
|
FE1
|
A:SF4602
|
2.7
|
23.6
|
1.0
|
FE3
|
A:SF4602
|
2.7
|
24.4
|
1.0
|
FE2
|
A:SF4602
|
2.7
|
21.7
|
1.0
|
HA
|
A:CYS503
|
2.8
|
31.7
|
1.0
|
CB
|
A:CYS503
|
3.3
|
22.4
|
1.0
|
CA
|
A:CYS503
|
3.4
|
26.4
|
1.0
|
HB3
|
A:CYS503
|
3.5
|
26.8
|
1.0
|
C3
|
A:402601
|
3.8
|
19.5
|
1.0
|
O3
|
A:402601
|
3.9
|
32.6
|
1.0
|
S4
|
A:SF4602
|
3.9
|
22.1
|
1.0
|
N
|
A:CYS503
|
4.0
|
24.2
|
1.0
|
HB2
|
A:CYS355
|
4.0
|
37.0
|
1.0
|
FE1
|
A:402601
|
4.0
|
26.4
|
1.0
|
H
|
A:CYS503
|
4.1
|
29.0
|
1.0
|
HB2
|
A:CYS503
|
4.1
|
26.8
|
1.0
|
S2
|
A:402601
|
4.2
|
25.8
|
1.0
|
C2
|
A:402601
|
4.4
|
30.5
|
1.0
|
HB2
|
A:CYS499
|
4.4
|
19.1
|
1.0
|
HG2
|
A:PRO354
|
4.6
|
29.7
|
1.0
|
C
|
A:GLY502
|
4.6
|
25.7
|
1.0
|
HB2
|
A:CYS300
|
4.7
|
28.9
|
1.0
|
SD
|
A:MET497
|
4.7
|
30.5
|
1.0
|
C
|
A:CYS503
|
4.8
|
21.7
|
1.0
|
CB
|
A:CYS355
|
4.8
|
30.8
|
1.0
|
SG
|
A:CYS300
|
4.8
|
22.9
|
1.0
|
SG
|
A:CYS355
|
4.8
|
29.7
|
1.0
|
HA
|
A:CYS300
|
4.9
|
30.5
|
1.0
|
SG
|
A:CYS499
|
4.9
|
22.8
|
1.0
|
H
|
A:CYS499
|
4.9
|
31.1
|
1.0
|
O
|
A:GLY502
|
4.9
|
26.8
|
1.0
|
HA2
|
A:GLY506
|
4.9
|
25.6
|
1.0
|
HD2
|
A:PRO301
|
4.9
|
35.3
|
1.0
|
HB3
|
A:MET497
|
5.0
|
32.5
|
1.0
|
C4
|
A:402601
|
5.0
|
26.9
|
1.0
|
|
Iron binding site 7 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 7 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe603
b:24.5
occ:1.00
|
FE1
|
A:SF4603
|
0.0
|
24.5
|
1.0
|
S4
|
A:SF4603
|
2.3
|
28.1
|
1.0
|
S2
|
A:SF4603
|
2.3
|
25.4
|
1.0
|
S3
|
A:SF4603
|
2.3
|
29.2
|
1.0
|
SG
|
A:CYS193
|
2.4
|
24.4
|
1.0
|
H
|
A:CYS193
|
2.7
|
29.6
|
1.0
|
FE2
|
A:SF4603
|
2.7
|
22.6
|
1.0
|
FE4
|
A:SF4603
|
2.7
|
23.7
|
1.0
|
FE3
|
A:SF4603
|
2.7
|
24.3
|
1.0
|
HG21
|
A:THR161
|
3.2
|
27.6
|
1.0
|
H
|
A:GLY194
|
3.4
|
34.3
|
1.0
|
N
|
A:CYS193
|
3.4
|
24.7
|
1.0
|
CB
|
A:CYS193
|
3.6
|
21.5
|
1.0
|
H
|
A:GLN195
|
3.6
|
33.3
|
1.0
|
HB3
|
A:CYS193
|
3.8
|
25.8
|
1.0
|
HB2
|
A:LEU191
|
3.8
|
28.9
|
1.0
|
N
|
A:GLY194
|
3.9
|
28.6
|
1.0
|
S1
|
A:SF4603
|
3.9
|
28.9
|
1.0
|
H
|
A:LEU191
|
3.9
|
26.9
|
1.0
|
CA
|
A:CYS193
|
3.9
|
22.2
|
1.0
|
H
|
A:LEU192
|
3.9
|
26.3
|
1.0
|
HB2
|
A:GLN195
|
4.0
|
35.9
|
1.0
|
CG2
|
A:THR161
|
4.1
|
23.0
|
1.0
|
HG23
|
A:THR161
|
4.1
|
27.6
|
1.0
|
H
|
A:CYS196
|
4.2
|
27.8
|
1.0
|
N
|
A:LEU192
|
4.3
|
21.9
|
1.0
|
C
|
A:CYS193
|
4.3
|
27.3
|
1.0
|
N
|
A:GLN195
|
4.4
|
27.8
|
1.0
|
C
|
A:LEU192
|
4.4
|
26.7
|
1.0
|
HB2
|
A:CYS193
|
4.4
|
25.8
|
1.0
|
HA
|
A:LEU192
|
4.5
|
30.5
|
1.0
|
HG22
|
A:THR161
|
4.5
|
27.6
|
1.0
|
N
|
A:LEU191
|
4.6
|
22.4
|
1.0
|
SG
|
A:CYS190
|
4.6
|
28.2
|
1.0
|
CA
|
A:LEU192
|
4.7
|
25.4
|
1.0
|
CB
|
A:LEU191
|
4.7
|
24.1
|
1.0
|
SG
|
A:CYS196
|
4.7
|
23.9
|
1.0
|
HG
|
A:LEU191
|
4.8
|
24.8
|
1.0
|
SG
|
A:CYS157
|
4.8
|
23.3
|
1.0
|
C
|
A:LEU191
|
4.8
|
26.3
|
1.0
|
HA
|
A:CYS157
|
4.8
|
22.8
|
1.0
|
HA
|
A:CYS193
|
4.9
|
26.6
|
1.0
|
CA
|
A:GLY194
|
4.9
|
24.8
|
1.0
|
HD12
|
A:LEU191
|
4.9
|
28.1
|
1.0
|
CB
|
A:GLN195
|
4.9
|
29.9
|
1.0
|
HG2
|
A:GLN195
|
4.9
|
35.7
|
1.0
|
CA
|
A:LEU191
|
5.0
|
25.3
|
1.0
|
HB2
|
A:CYS157
|
5.0
|
26.7
|
1.0
|
|
Iron binding site 8 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 8 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe603
b:22.6
occ:1.00
|
FE2
|
A:SF4603
|
0.0
|
22.6
|
1.0
|
SG
|
A:CYS157
|
2.3
|
23.3
|
1.0
|
S4
|
A:SF4603
|
2.3
|
28.1
|
1.0
|
S3
|
A:SF4603
|
2.3
|
29.2
|
1.0
|
S1
|
A:SF4603
|
2.3
|
28.9
|
1.0
|
FE3
|
A:SF4603
|
2.7
|
24.3
|
1.0
|
FE1
|
A:SF4603
|
2.7
|
24.5
|
1.0
|
FE4
|
A:SF4603
|
2.7
|
23.7
|
1.0
|
HB2
|
A:CYS157
|
2.9
|
26.7
|
1.0
|
CB
|
A:CYS157
|
3.1
|
22.2
|
1.0
|
HB3
|
A:ALA165
|
3.3
|
28.8
|
1.0
|
HA
|
A:CYS157
|
3.6
|
22.8
|
1.0
|
H
|
A:MET166
|
3.8
|
31.3
|
1.0
|
HG1
|
A:THR163
|
3.8
|
30.4
|
1.0
|
HG21
|
A:THR161
|
3.9
|
27.6
|
1.0
|
S2
|
A:SF4603
|
3.9
|
25.4
|
1.0
|
HB3
|
A:CYS157
|
3.9
|
26.7
|
1.0
|
CA
|
A:CYS157
|
3.9
|
19.0
|
1.0
|
H
|
A:ALA165
|
4.0
|
31.7
|
1.0
|
HB2
|
A:MET166
|
4.0
|
30.9
|
1.0
|
HG1
|
A:THR161
|
4.0
|
33.2
|
1.0
|
OG1
|
A:THR161
|
4.2
|
27.6
|
1.0
|
OG1
|
A:THR163
|
4.2
|
25.4
|
1.0
|
HG23
|
A:THR161
|
4.2
|
27.6
|
1.0
|
CB
|
A:ALA165
|
4.3
|
24.0
|
1.0
|
N
|
A:MET166
|
4.4
|
26.1
|
1.0
|
HB3
|
A:MET166
|
4.5
|
30.9
|
1.0
|
CG2
|
A:THR161
|
4.5
|
23.0
|
1.0
|
HB3
|
A:CYS196
|
4.5
|
30.8
|
1.0
|
HB2
|
A:ALA165
|
4.5
|
28.8
|
1.0
|
N
|
A:ALA165
|
4.6
|
26.4
|
1.0
|
H
|
A:LEU191
|
4.6
|
26.9
|
1.0
|
CB
|
A:MET166
|
4.7
|
25.7
|
1.0
|
HA
|
A:CYS190
|
4.7
|
34.0
|
1.0
|
C
|
A:CYS157
|
4.8
|
23.8
|
1.0
|
H
|
A:CYS196
|
4.8
|
27.8
|
1.0
|
SG
|
A:CYS196
|
4.8
|
23.9
|
1.0
|
SG
|
A:CYS193
|
4.8
|
24.4
|
1.0
|
CA
|
A:ALA165
|
4.8
|
22.1
|
1.0
|
HB1
|
A:ALA165
|
4.8
|
28.8
|
1.0
|
O
|
A:CYS157
|
4.9
|
24.1
|
1.0
|
SG
|
A:CYS190
|
4.9
|
28.2
|
1.0
|
C
|
A:ALA165
|
4.9
|
24.6
|
1.0
|
CB
|
A:THR161
|
5.0
|
27.3
|
1.0
|
|
Iron binding site 9 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 9 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 9 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe603
b:24.3
occ:1.00
|
FE3
|
A:SF4603
|
0.0
|
24.3
|
1.0
|
SG
|
A:CYS196
|
2.3
|
23.9
|
1.0
|
S2
|
A:SF4603
|
2.3
|
25.4
|
1.0
|
S4
|
A:SF4603
|
2.3
|
28.1
|
1.0
|
S1
|
A:SF4603
|
2.3
|
28.9
|
1.0
|
FE2
|
A:SF4603
|
2.7
|
22.6
|
1.0
|
FE4
|
A:SF4603
|
2.7
|
23.7
|
1.0
|
FE1
|
A:SF4603
|
2.7
|
24.5
|
1.0
|
H
|
A:CYS196
|
3.1
|
27.8
|
1.0
|
HB3
|
A:CYS196
|
3.1
|
30.8
|
1.0
|
CB
|
A:CYS196
|
3.3
|
25.7
|
1.0
|
HE2
|
A:MET166
|
3.7
|
34.6
|
1.0
|
H
|
A:GLY194
|
3.8
|
34.3
|
1.0
|
HD11
|
A:LEU140
|
3.8
|
42.4
|
1.0
|
N
|
A:CYS196
|
3.9
|
23.2
|
1.0
|
S3
|
A:SF4603
|
3.9
|
29.2
|
1.0
|
HB2
|
A:CYS157
|
4.0
|
26.7
|
1.0
|
HB2
|
A:MET166
|
4.0
|
30.9
|
1.0
|
HB2
|
A:CYS196
|
4.1
|
30.8
|
1.0
|
HD13
|
A:LEU140
|
4.2
|
42.4
|
1.0
|
CA
|
A:CYS196
|
4.2
|
22.3
|
1.0
|
H
|
A:GLN195
|
4.3
|
33.3
|
1.0
|
HA2
|
A:GLY194
|
4.4
|
29.8
|
1.0
|
N
|
A:GLY194
|
4.5
|
28.6
|
1.0
|
CD1
|
A:LEU140
|
4.5
|
35.3
|
1.0
|
HE3
|
A:MET166
|
4.5
|
34.6
|
1.0
|
CE
|
A:MET166
|
4.6
|
28.9
|
1.0
|
N
|
A:GLN195
|
4.6
|
27.8
|
1.0
|
H
|
A:CYS193
|
4.6
|
29.6
|
1.0
|
HD21
|
A:LEU140
|
4.6
|
35.7
|
1.0
|
SG
|
A:CYS190
|
4.6
|
28.2
|
1.0
|
SG
|
A:CYS157
|
4.7
|
23.3
|
1.0
|
SG
|
A:CYS193
|
4.7
|
24.4
|
1.0
|
CB
|
A:CYS157
|
4.8
|
22.2
|
1.0
|
HB2
|
A:GLN195
|
4.8
|
35.9
|
1.0
|
HA
|
A:CYS196
|
4.8
|
26.8
|
1.0
|
CA
|
A:GLY194
|
4.8
|
24.8
|
1.0
|
H
|
A:ILE197
|
4.9
|
24.9
|
1.0
|
C
|
A:GLY194
|
4.9
|
22.2
|
1.0
|
HE1
|
A:MET166
|
5.0
|
34.6
|
1.0
|
CB
|
A:MET166
|
5.0
|
25.7
|
1.0
|
|
Iron binding site 10 out
of 40 in 6yf4
Go back to
Iron Binding Sites List in 6yf4
Iron binding site 10 out
of 40 in the [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 10 of [Fefe]-Hydrogenase I From Clostridium Pasteurianum (Cpi), Variant E279D within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe603
b:23.7
occ:1.00
|
FE4
|
A:SF4603
|
0.0
|
23.7
|
1.0
|
SG
|
A:CYS190
|
2.3
|
28.2
|
1.0
|
S2
|
A:SF4603
|
2.3
|
25.4
|
1.0
|
S1
|
A:SF4603
|
2.3
|
28.9
|
1.0
|
S3
|
A:SF4603
|
2.3
|
29.2
|
1.0
|
FE1
|
A:SF4603
|
2.7
|
24.5
|
1.0
|
FE3
|
A:SF4603
|
2.7
|
24.3
|
1.0
|
FE2
|
A:SF4603
|
2.7
|
22.6
|
1.0
|
H
|
A:LEU192
|
3.0
|
26.3
|
1.0
|
H
|
A:LEU191
|
3.2
|
26.9
|
1.0
|
HB3
|
A:ALA165
|
3.3
|
28.8
|
1.0
|
HA
|
A:CYS190
|
3.4
|
34.0
|
1.0
|
CB
|
A:CYS190
|
3.4
|
27.7
|
1.0
|
HB2
|
A:CYS190
|
3.5
|
33.2
|
1.0
|
HD11
|
A:LEU140
|
3.7
|
42.4
|
1.0
|
N
|
A:LEU191
|
3.7
|
22.4
|
1.0
|
N
|
A:LEU192
|
3.8
|
21.9
|
1.0
|
CA
|
A:CYS190
|
3.8
|
28.3
|
1.0
|
S4
|
A:SF4603
|
3.9
|
28.1
|
1.0
|
H
|
A:CYS193
|
3.9
|
29.6
|
1.0
|
HA
|
A:LEU192
|
4.0
|
30.5
|
1.0
|
HD2
|
A:PHE185
|
4.0
|
37.4
|
1.0
|
CB
|
A:ALA165
|
4.1
|
24.0
|
1.0
|
C
|
A:CYS190
|
4.2
|
24.8
|
1.0
|
HB1
|
A:ALA165
|
4.2
|
28.8
|
1.0
|
HB3
|
A:CYS190
|
4.2
|
33.2
|
1.0
|
CA
|
A:LEU192
|
4.4
|
25.4
|
1.0
|
HB2
|
A:ALA165
|
4.5
|
28.8
|
1.0
|
CD1
|
A:LEU140
|
4.5
|
35.3
|
1.0
|
HD23
|
A:LEU192
|
4.6
|
28.4
|
1.0
|
N
|
A:CYS193
|
4.6
|
24.7
|
1.0
|
H
|
A:GLY194
|
4.7
|
34.3
|
1.0
|
CD2
|
A:PHE185
|
4.7
|
31.1
|
1.0
|
HB2
|
A:LEU191
|
4.7
|
28.9
|
1.0
|
C
|
A:LEU191
|
4.7
|
26.3
|
1.0
|
HD12
|
A:LEU140
|
4.7
|
42.4
|
1.0
|
CA
|
A:LEU191
|
4.7
|
25.3
|
1.0
|
HD13
|
A:LEU140
|
4.7
|
42.4
|
1.0
|
SG
|
A:CYS157
|
4.8
|
23.3
|
1.0
|
SG
|
A:CYS196
|
4.8
|
23.9
|
1.0
|
HE2
|
A:PHE185
|
4.9
|
39.0
|
1.0
|
HB3
|
A:PHE185
|
4.9
|
26.5
|
1.0
|
HB2
|
A:MET166
|
5.0
|
30.9
|
1.0
|
|
Reference:
O.Lampret,
J.Duan,
E.Hofmann,
M.Winkler,
F.A.Armstrong,
T.Happe.
The Roles of Long-Range Proton-Coupled Electron Transfer in the Directionality and Efficiency of [Fefe]-Hydrogenases. Proc.Natl.Acad.Sci.Usa V. 117 20520 2020.
ISSN: ESSN 1091-6490
PubMed: 32796105
DOI: 10.1073/PNAS.2007090117
Page generated: Wed Aug 7 16:41:49 2024
|