Iron in PDB 8q25: Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.

All present enzymatic activity of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.:
1.6.5.3; 1.6.99.3; 7.1.1.2;

The structure of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. also contains other interesting chemical elements:

Potassium	(K)	1 atom
Zinc	(Zn)	2 atoms
Magnesium	(Mg)	1 atom

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 28;

Binding sites:

The binding sites of Iron atom in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. (pdb code 8q25). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 28 binding sites of Iron where determined in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng., PDB code: 8q25:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Iron binding site 1 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:23.0 occ:1.00 FE1 B:SF4201 0.0 23.0 1.0 SG B:CYS119 2.2 21.7 1.0 S3 B:SF4201 2.3 23.0 1.0 S4 B:SF4201 2.3 23.0 1.0 S2 B:SF4201 2.3 23.0 1.0 FE3 B:SF4201 2.7 23.0 1.0 FE2 B:SF4201 2.7 23.0 1.0 FE4 B:SF4201 2.7 23.0 1.0 CB B:CYS119 3.2 21.7 1.0 S1 B:SF4201 3.9 23.0 1.0 N B:CYS119 4.0 21.7 1.0 OG1 B:THR91 4.1 24.7 1.0 CA B:CYS119 4.2 21.7 1.0 SG B:CYS149 4.5 20.2 1.0 CQ2 D:2MR85 4.6 25.2 1.0 SG B:CYS54 4.8 24.6 1.0 SG B:CYS55 4.8 24.8 1.0 CE2 B:TYR126 4.8 24.4 1.0 N B:THR91 5.0 24.7 1.0

Iron binding site 2 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:23.0 occ:1.00 FE2 B:SF4201 0.0 23.0 1.0 SG B:CYS149 2.3 20.2 1.0 S1 B:SF4201 2.3 23.0 1.0 S4 B:SF4201 2.3 23.0 1.0 S3 B:SF4201 2.3 23.0 1.0 FE4 B:SF4201 2.7 23.0 1.0 FE1 B:SF4201 2.7 23.0 1.0 FE3 B:SF4201 2.7 23.0 1.0 CB B:CYS149 3.5 20.2 1.0 CA B:CYS149 3.6 20.2 1.0 C B:CYS149 3.8 20.2 1.0 S2 B:SF4201 3.9 23.0 1.0 O B:CYS149 4.1 20.2 1.0 NE D:ARG105 4.2 21.6 1.0 N B:PRO150 4.2 21.2 1.0 CD2 D:HIS190 4.5 21.5 1.0 NE2 D:HIS190 4.6 21.5 1.0 NH2 D:ARG105 4.6 21.6 1.0 CA B:PRO150 4.6 21.2 1.0 SG B:CYS55 4.7 24.8 1.0 SG B:CYS119 4.7 21.7 1.0 SG B:CYS54 4.8 24.6 1.0 CZ D:ARG105 4.8 21.6 1.0 CD B:PRO150 4.9 21.2 1.0 N B:CYS149 4.9 20.2 1.0

Iron binding site 3 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:23.0 occ:1.00 FE3 B:SF4201 0.0 23.0 1.0 SG B:CYS54 2.3 24.6 1.0 S1 B:SF4201 2.3 23.0 1.0 S4 B:SF4201 2.3 23.0 1.0 S2 B:SF4201 2.3 23.0 1.0 FE1 B:SF4201 2.7 23.0 1.0 FE4 B:SF4201 2.7 23.0 1.0 FE2 B:SF4201 2.7 23.0 1.0 CB B:CYS54 3.2 24.6 1.0 CQ2 D:2MR85 3.8 25.2 1.0 N B:CYS54 3.9 24.6 1.0 S3 B:SF4201 3.9 23.0 1.0 NE2 D:HIS190 4.0 21.5 1.0 CA B:CYS54 4.0 24.6 1.0 N B:CYS55 4.1 24.8 1.0 C B:CYS54 4.4 24.6 1.0 CG D:ARG105 4.4 21.6 1.0 CD2 D:HIS190 4.7 21.5 1.0 CB B:ALA53 4.7 26.6 1.0 SG B:CYS119 4.8 21.7 1.0 SG B:CYS55 4.8 24.8 1.0 NE D:ARG105 4.9 21.6 1.0 SG B:CYS149 4.9 20.2 1.0 NH2 D:2MR85 4.9 25.2 1.0 C B:ALA53 4.9 26.6 1.0 CD D:ARG105 5.0 21.6 1.0 CE1 D:HIS190 5.0 21.5 1.0

Iron binding site 4 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:23.0 occ:1.00 FE4 B:SF4201 0.0 23.0 1.0 SG B:CYS55 2.3 24.8 1.0 S2 B:SF4201 2.3 23.0 1.0 S3 B:SF4201 2.3 23.0 1.0 S1 B:SF4201 2.3 23.0 1.0 FE2 B:SF4201 2.7 23.0 1.0 FE3 B:SF4201 2.7 23.0 1.0 FE1 B:SF4201 2.7 23.0 1.0 CB B:CYS55 3.3 24.8 1.0 N B:CYS55 3.6 24.8 1.0 CA B:CYS55 3.8 24.8 1.0 S4 B:SF4201 3.9 23.0 1.0 CB B:PRO150 4.4 21.2 1.0 CA B:PRO150 4.5 21.2 1.0 C B:CYS54 4.5 24.6 1.0 SG B:CYS54 4.7 24.6 1.0 SG B:CYS149 4.8 20.2 1.0 CB B:CYS54 4.8 24.6 1.0 CA B:GLY117 4.8 21.4 1.0 SG B:CYS119 4.8 21.7 1.0 CA B:CYS149 4.8 20.2 1.0 N B:PRO150 4.8 21.2 1.0 CB B:CYS119 4.9 21.7 1.0 N B:CYS54 4.9 24.6 1.0 CA B:CYS54 5.0 24.6 1.0

Iron binding site 5 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe301 b:51.8 occ:1.00 FE1 E:FES301 0.0 51.8 1.0 S2 E:FES301 2.2 51.8 1.0 S1 E:FES301 2.2 51.8 1.0 SG E:CYS108 2.3 49.1 1.0 SG E:CYS103 2.3 50.5 1.0 FE2 E:FES301 2.7 51.8 1.0 CB E:CYS108 3.3 49.1 1.0 CB E:CYS103 3.3 50.5 1.0 N E:CYS108 4.2 49.1 1.0 CA E:CYS144 4.2 48.0 1.0 CA E:CYS108 4.3 49.1 1.0 N E:LEU145 4.4 50.9 1.0 SG E:CYS144 4.6 48.0 1.0 CA E:CYS103 4.7 50.5 1.0 CB E:CYS144 4.7 48.0 1.0 SG E:CYS148 4.8 50.8 1.0 CB E:THR105 4.8 45.5 1.0 OG1 E:THR105 4.8 45.5 1.0 CA E:CYS148 4.9 50.8 1.0 N E:THR105 4.9 45.5 1.0 CG E:MET153 4.9 54.9 1.0 C E:CYS103 4.9 50.5 1.0 C E:CYS144 4.9 48.0 1.0

Iron binding site 6 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe301 b:51.8 occ:1.00 FE2 E:FES301 0.0 51.8 1.0 S2 E:FES301 2.2 51.8 1.0 S1 E:FES301 2.2 51.8 1.0 SG E:CYS148 2.3 50.8 1.0 SG E:CYS144 2.3 48.0 1.0 FE1 E:FES301 2.7 51.8 1.0 CB E:CYS144 3.3 48.0 1.0 CB E:CYS148 3.3 50.8 1.0 CA E:CYS148 3.6 50.8 1.0 CA E:CYS144 3.7 48.0 1.0 N E:CYS148 3.7 50.8 1.0 N E:LEU145 4.0 50.9 1.0 C E:CYS144 4.3 48.0 1.0 N E:ALA147 4.3 52.1 1.0 N E:GLY146 4.3 49.8 1.0 SG E:CYS103 4.3 50.5 1.0 C E:ALA147 4.4 52.1 1.0 SG E:CYS108 4.5 49.1 1.0 N F:GLY103 4.6 47.9 1.0 O E:ALA147 4.9 52.1 1.0 CA E:GLY146 4.9 49.8 1.0 CB E:PRO107 5.0 47.3 1.0 CA E:ALA147 5.0 52.1 1.0 N E:CYS144 5.0 48.0 1.0

Iron binding site 7 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe501 b:31.2 occ:1.00 FE1 F:SF4501 0.0 31.2 1.0 SG F:CYS359 2.3 33.1 1.0 S3 F:SF4501 2.3 31.2 1.0 S2 F:SF4501 2.3 31.2 1.0 S4 F:SF4501 2.3 31.2 1.0 FE2 F:SF4501 2.7 31.2 1.0 FE4 F:SF4501 2.7 31.2 1.0 FE3 F:SF4501 2.7 31.2 1.0 CB F:CYS359 3.3 33.1 1.0 N F:CYS359 3.8 33.1 1.0 S1 F:SF4501 3.9 31.2 1.0 CA F:CYS359 3.9 33.1 1.0 N F:GLN361 3.9 30.7 1.0 CB F:GLN361 4.0 30.7 1.0 N F:GLY360 4.1 31.0 1.0 C F:CYS359 4.2 33.1 1.0 CD F:PRO203 4.3 35.8 1.0 CA F:GLN361 4.5 30.7 1.0 N F:CYS362 4.6 28.9 1.0 NE2 F:GLN361 4.6 30.7 1.0 SG F:CYS365 4.8 29.9 1.0 CG F:PRO203 4.8 35.8 1.0 SG F:CYS405 4.8 32.7 1.0 OG F:SER358 4.9 33.5 1.0 SG F:CYS362 4.9 28.9 1.0 C F:GLY360 5.0 31.0 1.0 O F:CYS359 5.0 33.1 1.0

Iron binding site 8 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe501 b:31.2 occ:1.00 FE2 F:SF4501 0.0 31.2 1.0 SG F:CYS365 2.2 29.9 1.0 S1 F:SF4501 2.3 31.2 1.0 S3 F:SF4501 2.3 31.2 1.0 S4 F:SF4501 2.3 31.2 1.0 FE4 F:SF4501 2.7 31.2 1.0 FE1 F:SF4501 2.7 31.2 1.0 FE3 F:SF4501 2.7 31.2 1.0 CB F:CYS365 3.0 29.9 1.0 OG F:SER358 3.4 33.5 1.0 S2 F:SF4501 3.9 31.2 1.0 N F:CYS359 4.3 33.1 1.0 CA F:CYS365 4.3 29.9 1.0 CB F:SER358 4.5 33.5 1.0 SG F:CYS362 4.5 28.9 1.0 C F:CYS365 4.6 29.9 1.0 CD2 F:LEU407 4.6 36.4 1.0 CA F:SER358 4.7 33.5 1.0 SG F:CYS405 4.7 32.7 1.0 SG F:CYS359 4.8 33.1 1.0 CB F:LEU407 4.9 36.4 1.0 N F:ARG366 4.9 28.4 1.0 N F:GLY360 4.9 31.0 1.0 N F:GLY408 4.9 35.3 1.0 C F:SER358 5.0 33.5 1.0

Iron binding site 9 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 9 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe501 b:31.2 occ:1.00 FE3 F:SF4501 0.0 31.2 1.0 S1 F:SF4501 2.3 31.2 1.0 S4 F:SF4501 2.3 31.2 1.0 S2 F:SF4501 2.3 31.2 1.0 SG F:CYS405 2.3 32.7 1.0 FE2 F:SF4501 2.7 31.2 1.0 FE1 F:SF4501 2.7 31.2 1.0 FE4 F:SF4501 2.7 31.2 1.0 CB F:CYS405 3.4 32.7 1.0 S3 F:SF4501 3.9 31.2 1.0 N F:CYS405 4.0 32.7 1.0 CA F:CYS405 4.1 32.7 1.0 CB F:LEU407 4.2 36.4 1.0 CG F:PRO203 4.3 35.8 1.0 C F:CYS405 4.4 32.7 1.0 O F:CYS405 4.5 32.7 1.0 CD1 F:ILE185 4.5 38.0 1.0 CD F:PRO203 4.7 35.8 1.0 N F:LEU407 4.8 36.4 1.0 SG F:CYS365 4.8 29.9 1.0 SG F:CYS359 4.8 33.1 1.0 SG F:CYS362 4.8 28.9 1.0 N F:GLY408 4.9 35.3 1.0

Iron binding site 10 out of 28 in the Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 10 of Outward-Facing, OPEN1 Proteoliposome Complex I at 2.8 A, After Deactivation Treatment. Initially Purified in Lmng. within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe501 b:31.2 occ:1.00 FE4 F:SF4501 0.0 31.2 1.0 S3 F:SF4501 2.3 31.2 1.0 S1 F:SF4501 2.3 31.2 1.0 S2 F:SF4501 2.3 31.2 1.0 SG F:CYS362 2.3 28.9 1.0 FE2 F:SF4501 2.7 31.2 1.0 FE1 F:SF4501 2.7 31.2 1.0 FE3 F:SF4501 2.7 31.2 1.0 CB F:CYS362 3.4 28.9 1.0 N F:CYS362 3.7 28.9 1.0 S4 F:SF4501 3.9 31.2 1.0 CA F:CYS362 4.0 28.9 1.0 O F:CYS362 4.2 28.9 1.0 CB F:CYS365 4.3 29.9 1.0 N F:ILE404 4.3 30.4 1.0 CB F:ILE404 4.4 30.4 1.0 C F:CYS362 4.5 28.9 1.0 SG F:CYS365 4.6 29.9 1.0 CG1 F:ILE404 4.6 30.4 1.0 CB F:GLN361 4.7 30.7 1.0 CB F:THR403 4.7 30.3 1.0 N F:CYS405 4.7 32.7 1.0 SG F:CYS359 4.8 33.1 1.0 C F:GLN361 4.8 30.7 1.0 SG F:CYS405 4.9 32.7 1.0 CD1 F:ILE404 4.9 30.4 1.0 N F:GLN361 4.9 30.7 1.0 CA F:ILE404 4.9 30.4 1.0

D.N.Grba, J.J.Wright, W.Fisher, Z.Yin, J.Hirst. Structure of Complex I Embedded in Liposomes Reveals Mechanism of Active/Deactive Transition Science 2024.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.ADO2075