Iron in PDB 8tlq: Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex

All present enzymatic activity of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex:
2.7.7.7;

The structure of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Iron atom in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex (pdb code 8tlq). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex, PDB code: 8tlq:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1603 b:53.7 occ:1.00 FE1 A:SF41603 0.0 53.7 1.0 S4 A:SF41603 2.1 53.7 1.0 S3 A:SF41603 2.1 53.7 1.0 S2 A:SF41603 2.1 53.7 1.0 SG A:CYS1473 2.3 56.0 1.0 FE2 A:SF41603 3.0 53.7 1.0 FE3 A:SF41603 3.0 53.7 1.0 FE4 A:SF41603 3.0 53.7 1.0 CB A:CYS1473 3.2 56.0 1.0 S1 A:SF41603 3.7 53.7 1.0 CB A:VAL1475 3.8 52.7 1.0 N A:PHE1476 4.3 53.2 1.0 CG2 A:VAL1475 4.3 52.7 1.0 CG1 A:VAL1475 4.5 52.7 1.0 CG A:ARG852 4.6 53.1 1.0 CA A:CYS1473 4.6 56.0 1.0 CA A:VAL1475 4.6 52.7 1.0 N A:VAL1475 4.7 52.7 1.0 CD A:ARG852 4.7 53.1 1.0 C A:VAL1475 4.8 52.7 1.0 C A:CYS1473 4.9 56.0 1.0 CB A:PHE1476 4.9 53.2 1.0 O A:CYS1473 4.9 56.0 1.0 SG A:CYS1449 5.0 53.2 1.0

Iron binding site 2 out of 4 in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1603 b:53.7 occ:1.00 FE2 A:SF41603 0.0 53.7 1.0 S3 A:SF41603 2.1 53.7 1.0 S4 A:SF41603 2.1 53.7 1.0 S1 A:SF41603 2.1 53.7 1.0 SG A:CYS1449 2.3 53.2 1.0 CB A:CYS1449 2.8 53.2 1.0 FE3 A:SF41603 3.0 53.7 1.0 FE4 A:SF41603 3.0 53.7 1.0 FE1 A:SF41603 3.0 53.7 1.0 S2 A:SF41603 3.7 53.7 1.0 CG A:PRO854 3.8 47.9 1.0 CA A:CYS1449 4.3 53.2 1.0 CB A:PRO854 4.4 47.9 1.0 CG A:ARG852 4.4 53.1 1.0 CD A:PRO854 4.5 47.9 1.0 C A:CYS1449 4.6 53.2 1.0 O A:CYS1446 4.7 52.8 1.0 CA A:PRO854 4.8 47.9 1.0 CA A:CYS1446 4.8 52.8 1.0 N A:PRO854 4.8 47.9 1.0 N A:SER1450 4.9 53.2 1.0 SG A:CYS1473 5.0 56.0 1.0

Iron binding site 3 out of 4 in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1603 b:53.7 occ:1.00 FE3 A:SF41603 0.0 53.7 1.0 S1 A:SF41603 2.1 53.7 1.0 S4 A:SF41603 2.1 53.7 1.0 S2 A:SF41603 2.1 53.7 1.0 SG A:CYS1468 2.3 49.7 1.0 FE2 A:SF41603 3.0 53.7 1.0 FE4 A:SF41603 3.0 53.7 1.0 FE1 A:SF41603 3.0 53.7 1.0 CB A:CYS1468 3.4 49.7 1.0 S3 A:SF41603 3.7 53.7 1.0 CB A:PRO854 3.9 47.9 1.0 CA A:PRO854 4.1 47.9 1.0 CG A:PRO854 4.4 47.9 1.0 CB A:SER1470 4.6 53.5 1.0 CA A:CYS1468 4.7 49.7 1.0 C A:CYS1468 4.9 49.7 1.0 N A:SER1470 4.9 53.5 1.0 SG A:CYS1446 4.9 52.8 1.0 N A:HIS855 5.0 49.4 1.0 N A:PRO854 5.0 47.9 1.0

Iron binding site 4 out of 4 in the Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Cryo-Em Structure of the REV1-Polzeta-Dna-Dctp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1603 b:53.7 occ:1.00 FE4 A:SF41603 0.0 53.7 1.0 S1 A:SF41603 2.1 53.7 1.0 S2 A:SF41603 2.1 53.7 1.0 S3 A:SF41603 2.1 53.7 1.0 SG A:CYS1446 2.3 52.8 1.0 FE2 A:SF41603 3.0 53.7 1.0 FE3 A:SF41603 3.0 53.7 1.0 FE1 A:SF41603 3.0 53.7 1.0 CB A:CYS1446 3.3 52.8 1.0 CA A:CYS1446 3.5 52.8 1.0 S4 A:SF41603 3.7 53.7 1.0 CG1 A:VAL1475 4.0 52.7 1.0 CB A:VAL1475 4.3 52.7 1.0 N A:CYS1446 4.3 52.8 1.0 C A:CYS1446 4.6 52.8 1.0 O A:CYS1446 4.6 52.8 1.0 CB A:CYS1449 4.8 53.2 1.0 SG A:CYS1468 4.9 49.7 1.0 N A:PHE1476 5.0 53.2 1.0

R.Malik, R.E.Johnson, I.B.Ubarretxena, L.Prakash, S.Prakash, A.K.Aggarwal. Cryo-Em Structure of the REV1-Polzeta Holocomplex Reveals the Mechanism of Their Cooperativity in Translesion Dna Synthesis Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985