Iron in PDB 8ty0: Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin

The structure of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin, PDB code: 8ty0 was solved by A.H.Follmer, R.P.Jakob, M.Mukherjee, T.R.Ward, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.11 / 1.54
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	192.487, 57.72, 57.77, 90, 107.44, 90
R / Rfree (%)	17.4 / 19.1

The binding sites of Iron atom in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin (pdb code 8ty0). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 5 binding sites of Iron where determined in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin, PDB code: 8ty0:
Jump to Iron binding site number: 1; 2; 3; 4; 5;

Iron binding site 1 out of 5 in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe201 b:54.4 occ:0.44 FE1 C:SIK201 0.0 54.4 0.4 FE1 C:SIK201 1.1 43.8 0.2 FE1 C:SIK201 1.3 46.6 0.3 N7 C:SIK201 1.7 41.0 0.3 N4 C:SIK201 1.8 42.0 0.2 N8 C:SIK201 2.1 44.9 0.4 N7 C:SIK201 2.1 46.8 0.4 N6 C:SIK201 2.1 44.8 0.4 N4 C:SIK201 2.1 46.2 0.4 N6 C:SIK201 2.1 38.9 0.2 N8 C:SIK201 2.2 36.4 0.3 C22 C:SIK201 2.5 41.4 0.3 N8 C:SIK201 2.6 39.2 0.2 C21 C:SIK201 2.7 35.6 0.3 C34 C:SIK201 2.7 39.6 0.2 N6 C:SIK201 2.8 34.1 0.3 N7 C:SIK201 2.8 42.5 0.2 C26 C:SIK201 2.8 42.5 0.3 C16 C:SIK201 2.9 44.3 0.2 C31 C:SIK201 2.9 36.2 0.2 C25 C:SIK201 3.0 44.1 0.3 C31 C:SIK201 3.0 38.5 0.4 C17 C:SIK201 3.0 33.2 0.4 C29 C:SIK201 3.0 33.5 0.4 C22 C:SIK201 3.1 39.1 0.4 C21 C:SIK201 3.1 42.9 0.4 C26 C:SIK201 3.1 44.2 0.4 C34 C:SIK201 3.1 37.5 0.4 C16 C:SIK201 3.1 46.0 0.4 C15 C:SIK201 3.1 42.5 0.2 N4 C:SIK201 3.1 40.3 0.3 C17 C:SIK201 3.2 40.8 0.2 C29 C:SIK201 3.3 42.3 0.3 C20 C:SIK201 3.4 31.8 0.3 C29 C:SIK201 3.4 34.7 0.2 C17 C:SIK201 3.4 32.0 0.3 C25 C:SIK201 3.4 41.3 0.4 C30 C:SIK201 3.4 42.2 0.4 C20 C:SIK201 3.5 43.6 0.4 C30 C:SIK201 3.5 31.4 0.2 C15 C:SIK201 3.5 42.5 0.4 C23 C:SIK201 3.6 35.7 0.3 C21 C:SIK201 3.7 39.6 0.2 C24 C:SIK201 3.7 35.1 0.3 C26 C:SIK201 3.7 39.9 0.2 C22 C:SIK201 3.8 39.7 0.2 C20 C:SIK201 3.9 39.7 0.2 C33 C:SIK201 3.9 34.7 0.2 C31 C:SIK201 3.9 39.8 0.3 C32 C:SIK201 4.0 33.9 0.2 C16 C:SIK201 4.0 35.0 0.3 C30 C:SIK201 4.0 36.9 0.3 C27 C:SIK201 4.1 40.3 0.3 C19 C:SIK201 4.1 41.0 0.2 C34 C:SIK201 4.2 35.8 0.3 C32 C:SIK201 4.2 38.6 0.4 C18 C:SIK201 4.2 43.0 0.4 C19 C:SIK201 4.2 39.7 0.4 C27 C:SIK201 4.2 37.5 0.4 C23 C:SIK201 4.2 39.4 0.4 C25 C:SIK201 4.2 39.1 0.2 C28 C:SIK201 4.2 39.6 0.4 C33 C:SIK201 4.2 41.8 0.4 C24 C:SIK201 4.3 41.3 0.4 C18 C:SIK201 4.3 41.2 0.2 C28 C:SIK201 4.3 42.6 0.3 H25 C:SIK201 4.4 38.1 0.3 C35 C:SIK201 4.4 37.0 0.3 H30 C:SIK201 4.5 50.7 0.4 C14 C:SIK201 4.5 37.8 0.2 H33 C:SIK201 4.5 44.3 0.3 H25 C:SIK201 4.5 52.3 0.4 H30 C:SIK201 4.5 37.6 0.2 H26 C:SIK201 4.6 42.8 0.3 C15 C:SIK201 4.6 35.8 0.3 C28 C:SIK201 4.7 35.4 0.2 H27 C:SIK201 4.7 42.1 0.3 H21 C:SIK201 4.7 45.3 0.2 C18 C:SIK201 4.7 30.5 0.3 H33 C:SIK201 4.7 48.2 0.4 C35 C:SIK201 4.7 40.2 0.4 C27 C:SIK201 4.8 38.4 0.2 H32 C:SIK201 4.8 41.6 0.2 H22 C:SIK201 4.8 45.3 0.2 H34 C:SIK201 4.9 44.3 0.3 H25 C:SIK201 4.9 47.6 0.2 C24 C:SIK201 4.9 39.1 0.2 H31 C:SIK201 4.9 40.6 0.2 C14 C:SIK201 4.9 38.9 0.4 H28 C:SIK201 5.0 48.3 0.3 C19 C:SIK201 5.0 35.0 0.3 C23 C:SIK201 5.0 36.6 0.2

Iron binding site 2 out of 5 in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe201 b:43.8 occ:0.24 FE1 C:SIK201 0.0 43.8 0.2 N7 C:SIK201 1.0 41.0 0.3 FE1 C:SIK201 1.1 54.4 0.4 N7 C:SIK201 1.5 46.8 0.4 C21 C:SIK201 1.7 35.6 0.3 FE1 C:SIK201 1.9 46.6 0.3 C22 C:SIK201 2.0 41.4 0.3 N4 C:SIK201 2.1 42.0 0.2 N7 C:SIK201 2.1 42.5 0.2 N6 C:SIK201 2.1 38.9 0.2 N8 C:SIK201 2.1 39.2 0.2 N6 C:SIK201 2.3 44.8 0.4 C22 C:SIK201 2.4 39.1 0.4 N8 C:SIK201 2.4 44.9 0.4 C21 C:SIK201 2.5 42.9 0.4 C20 C:SIK201 2.5 31.8 0.3 C24 C:SIK201 2.7 35.1 0.3 N6 C:SIK201 2.8 34.1 0.3 C23 C:SIK201 2.8 35.7 0.3 C25 C:SIK201 2.9 41.3 0.4 C25 C:SIK201 2.9 44.1 0.3 C17 C:SIK201 3.0 32.0 0.3 N8 C:SIK201 3.0 36.4 0.3 C17 C:SIK201 3.0 33.2 0.4 N4 C:SIK201 3.0 46.2 0.4 C26 C:SIK201 3.0 44.2 0.4 C31 C:SIK201 3.1 36.2 0.2 C29 C:SIK201 3.1 34.7 0.2 C22 C:SIK201 3.1 39.7 0.2 C21 C:SIK201 3.1 39.6 0.2 C17 C:SIK201 3.1 40.8 0.2 C34 C:SIK201 3.1 39.6 0.2 C16 C:SIK201 3.1 44.3 0.2 C26 C:SIK201 3.1 39.9 0.2 C20 C:SIK201 3.2 43.6 0.4 C26 C:SIK201 3.3 42.5 0.3 C16 C:SIK201 3.5 46.0 0.4 H25 C:SIK201 3.5 38.1 0.3 C30 C:SIK201 3.5 31.4 0.2 C20 C:SIK201 3.5 39.7 0.2 C25 C:SIK201 3.5 39.1 0.2 C15 C:SIK201 3.5 42.5 0.2 C23 C:SIK201 3.6 39.4 0.4 C24 C:SIK201 3.6 41.3 0.4 H27 C:SIK201 3.6 42.1 0.3 C29 C:SIK201 3.6 33.5 0.4 H26 C:SIK201 3.8 42.8 0.3 C34 C:SIK201 3.9 37.5 0.4 N4 C:SIK201 3.9 40.3 0.3 H33 C:SIK201 3.9 48.2 0.4 C31 C:SIK201 4.0 38.5 0.4 C15 C:SIK201 4.1 42.5 0.4 C35 C:SIK201 4.1 40.2 0.4 C16 C:SIK201 4.1 35.0 0.3 H25 C:SIK201 4.2 52.3 0.4 C29 C:SIK201 4.2 42.3 0.3 H33 C:SIK201 4.2 44.3 0.3 C35 C:SIK201 4.2 37.0 0.3 C18 C:SIK201 4.2 43.0 0.4 C30 C:SIK201 4.3 42.2 0.4 C32 C:SIK201 4.3 33.9 0.2 C23 C:SIK201 4.3 36.6 0.2 C33 C:SIK201 4.3 34.7 0.2 C27 C:SIK201 4.3 38.4 0.2 C19 C:SIK201 4.3 41.0 0.2 C18 C:SIK201 4.3 30.5 0.3 C28 C:SIK201 4.3 35.4 0.2 C24 C:SIK201 4.3 39.1 0.2 C18 C:SIK201 4.3 41.2 0.2 C27 C:SIK201 4.4 37.5 0.4 C19 C:SIK201 4.4 39.7 0.4 H26 C:SIK201 4.5 47.2 0.4 H34 C:SIK201 4.5 48.2 0.4 H30 C:SIK201 4.6 37.6 0.2 H25 C:SIK201 4.6 47.6 0.2 H27 C:SIK201 4.6 49.5 0.4 C27 C:SIK201 4.6 40.3 0.3 C28 C:SIK201 4.7 39.6 0.4 C19 C:SIK201 4.8 35.0 0.3 C34 C:SIK201 4.8 35.8 0.3 C31 C:SIK201 4.9 39.8 0.3 ND1 C:HIS121 4.9 21.0 1.0 H34 C:SIK201 4.9 44.3 0.3 C15 C:SIK201 5.0 35.8 0.3 H23 C:SIK201 5.0 36.5 0.3

Iron binding site 3 out of 5 in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe201 b:46.6 occ:0.32 FE1 C:SIK201 0.0 46.6 0.3 N4 C:SIK201 0.8 42.0 0.2 N4 C:SIK201 1.3 46.2 0.4 FE1 C:SIK201 1.3 54.4 0.4 C31 C:SIK201 1.7 36.2 0.2 C34 C:SIK201 1.7 39.6 0.2 FE1 C:SIK201 1.9 43.8 0.2 N7 C:SIK201 2.1 41.0 0.3 N4 C:SIK201 2.1 40.3 0.3 N8 C:SIK201 2.1 36.4 0.3 N6 C:SIK201 2.1 34.1 0.3 C34 C:SIK201 2.2 37.5 0.4 C31 C:SIK201 2.3 38.5 0.4 N8 C:SIK201 2.5 44.9 0.4 N6 C:SIK201 2.5 44.8 0.4 C32 C:SIK201 2.6 33.9 0.2 C33 C:SIK201 2.7 34.7 0.2 C30 C:SIK201 2.7 31.4 0.2 C15 C:SIK201 2.7 42.5 0.2 C15 C:SIK201 2.9 42.5 0.4 N6 C:SIK201 2.9 38.9 0.2 N8 C:SIK201 2.9 39.2 0.2 C16 C:SIK201 3.0 46.0 0.4 C21 C:SIK201 3.0 35.6 0.3 C31 C:SIK201 3.1 39.8 0.3 C17 C:SIK201 3.1 32.0 0.3 C29 C:SIK201 3.1 42.3 0.3 C30 C:SIK201 3.1 42.2 0.4 C29 C:SIK201 3.1 33.5 0.4 C29 C:SIK201 3.1 34.7 0.2 C34 C:SIK201 3.1 35.8 0.3 C22 C:SIK201 3.1 41.4 0.3 C16 C:SIK201 3.1 44.3 0.2 C26 C:SIK201 3.1 42.5 0.3 C16 C:SIK201 3.1 35.0 0.3 N7 C:SIK201 3.3 46.8 0.4 C33 C:SIK201 3.3 41.8 0.4 C32 C:SIK201 3.4 38.6 0.4 C20 C:SIK201 3.4 31.8 0.3 C30 C:SIK201 3.5 36.9 0.3 C25 C:SIK201 3.6 44.1 0.3 C15 C:SIK201 3.6 35.8 0.3 H30 C:SIK201 3.6 37.6 0.2 H31 C:SIK201 3.6 40.6 0.2 H32 C:SIK201 3.7 41.6 0.2 C26 C:SIK201 3.7 44.2 0.4 C17 C:SIK201 3.8 33.2 0.4 N7 C:SIK201 4.0 42.5 0.2 C14 C:SIK201 4.0 37.8 0.2 H22 C:SIK201 4.1 45.3 0.2 C14 C:SIK201 4.1 38.9 0.4 H30 C:SIK201 4.1 50.7 0.4 H22 C:SIK201 4.1 46.6 0.4 C17 C:SIK201 4.2 40.8 0.2 C22 C:SIK201 4.2 39.1 0.4 C26 C:SIK201 4.2 39.9 0.2 C25 C:SIK201 4.2 41.3 0.4 C24 C:SIK201 4.3 35.1 0.3 C32 C:SIK201 4.3 37.9 0.3 C33 C:SIK201 4.3 34.5 0.3 C21 C:SIK201 4.3 42.9 0.4 H32 C:SIK201 4.3 50.1 0.4 C23 C:SIK201 4.3 35.7 0.3 C28 C:SIK201 4.3 42.6 0.3 C18 C:SIK201 4.3 30.5 0.3 C27 C:SIK201 4.3 40.3 0.3 C19 C:SIK201 4.3 35.0 0.3 C19 C:SIK201 4.4 39.7 0.4 H31 C:SIK201 4.4 46.3 0.4 C28 C:SIK201 4.4 39.6 0.4 H21 C:SIK201 4.4 45.3 0.2 C28 C:SIK201 4.4 35.4 0.2 H21 C:SIK201 4.5 46.6 0.4 C19 C:SIK201 4.5 41.0 0.2 H25 C:SIK201 4.5 38.1 0.3 C20 C:SIK201 4.5 43.6 0.4 H30 C:SIK201 4.5 44.2 0.3 C27 C:SIK201 4.6 37.5 0.4 C18 C:SIK201 4.7 43.0 0.4 C21 C:SIK201 4.9 39.6 0.2 C22 C:SIK201 4.9 39.7 0.2 C27 C:SIK201 5.0 38.4 0.2 CD2 B:HIS121 5.0 19.1 1.0

Iron binding site 4 out of 5 in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe201 b:55.5 occ:0.44 FE1 D:SIK201 0.0 55.5 0.4 FE1 D:SIK201 1.4 68.3 0.6 N8 D:SIK201 1.4 53.7 0.6 N7 D:SIK201 2.0 51.1 0.6 N6 D:SIK201 2.1 53.8 0.4 N8 D:SIK201 2.1 51.9 0.4 N4 D:SIK201 2.1 52.7 0.4 N7 D:SIK201 2.1 40.1 0.4 C26 D:SIK201 2.1 50.5 0.6 C22 D:SIK201 2.5 55.9 0.6 C29 D:SIK201 2.5 32.2 0.6 C25 D:SIK201 2.6 52.8 0.6 N4 D:SIK201 3.0 42.1 0.6 C17 D:SIK201 3.1 39.9 0.4 C31 D:SIK201 3.1 52.5 0.4 C29 D:SIK201 3.1 57.4 0.4 C21 D:SIK201 3.1 33.9 0.4 C26 D:SIK201 3.1 48.9 0.4 C16 D:SIK201 3.1 50.0 0.4 C22 D:SIK201 3.1 39.6 0.4 C34 D:SIK201 3.1 49.7 0.4 C21 D:SIK201 3.2 47.1 0.6 N6 D:SIK201 3.3 50.4 0.6 C27 D:SIK201 3.3 39.9 0.6 C30 D:SIK201 3.4 29.2 0.6 C20 D:SIK201 3.5 32.8 0.4 C28 D:SIK201 3.5 37.8 0.6 C30 D:SIK201 3.5 54.5 0.4 C25 D:SIK201 3.5 45.0 0.4 C31 D:SIK201 3.6 30.1 0.6 C15 D:SIK201 3.6 50.7 0.4 C23 D:SIK201 3.8 50.2 0.6 C35 D:SIK201 4.0 49.6 0.6 C17 D:SIK201 4.0 42.5 0.6 C20 D:SIK201 4.0 44.7 0.6 C24 D:SIK201 4.1 50.4 0.6 C34 D:SIK201 4.2 40.4 0.6 H28 D:SIK201 4.2 47.8 0.6 H33 D:SIK201 4.3 59.4 0.6 C18 D:SIK201 4.3 40.2 0.4 C27 D:SIK201 4.3 50.8 0.4 C28 D:SIK201 4.3 54.3 0.4 C32 D:SIK201 4.3 51.7 0.4 C33 D:SIK201 4.3 54.2 0.4 C19 D:SIK201 4.3 42.3 0.4 C24 D:SIK201 4.3 33.9 0.4 C23 D:SIK201 4.3 37.7 0.4 C16 D:SIK201 4.3 41.8 0.6 H30 D:SIK201 4.4 35.0 0.6 H34 D:SIK201 4.4 59.4 0.6 H29 D:SIK201 4.4 45.4 0.6 H25 D:SIK201 4.5 39.3 0.4 H30 D:SIK201 4.5 65.4 0.4 H26 D:SIK201 4.6 60.2 0.6 C15 D:SIK201 4.7 43.0 0.6 C32 D:SIK201 4.9 26.0 0.6 H35 D:SIK201 4.9 57.9 0.6 OE2 D:GLU112 4.9 37.1 0.5

Iron binding site 5 out of 5 in the Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Streptavidin Variant S112E-K121H Bound to Bis-Biotinylated Iron- Porphyrin within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe201 b:68.3 occ:0.56 FE1 D:SIK201 0.0 68.3 0.6 N6 D:SIK201 0.8 53.8 0.4 FE1 D:SIK201 1.4 55.5 0.4 C17 D:SIK201 1.8 39.9 0.4 C16 D:SIK201 2.0 50.0 0.4 N8 D:SIK201 2.1 53.7 0.6 N6 D:SIK201 2.1 50.4 0.6 N7 D:SIK201 2.1 51.1 0.6 N4 D:SIK201 2.1 42.1 0.6 N7 D:SIK201 2.4 40.1 0.4 N4 D:SIK201 2.5 52.7 0.4 C20 D:SIK201 2.6 32.8 0.4 C21 D:SIK201 2.8 33.9 0.4 C15 D:SIK201 2.9 50.7 0.4 C18 D:SIK201 2.9 40.2 0.4 C19 D:SIK201 3.0 42.3 0.4 C29 D:SIK201 3.0 32.2 0.6 C34 D:SIK201 3.0 49.7 0.4 C17 D:SIK201 3.0 42.5 0.6 C21 D:SIK201 3.1 47.1 0.6 C16 D:SIK201 3.1 41.8 0.6 C31 D:SIK201 3.1 30.1 0.6 C26 D:SIK201 3.1 50.5 0.6 C34 D:SIK201 3.1 40.4 0.6 C22 D:SIK201 3.1 55.9 0.6 N8 D:SIK201 3.4 51.9 0.4 C20 D:SIK201 3.4 44.7 0.6 C30 D:SIK201 3.5 29.2 0.6 C15 D:SIK201 3.5 43.0 0.6 C25 D:SIK201 3.6 52.8 0.6 H25 D:SIK201 3.6 39.3 0.4 C22 D:SIK201 3.7 39.6 0.4 C31 D:SIK201 3.8 52.5 0.4 H23 D:SIK201 3.9 48.2 0.4 H24 D:SIK201 4.0 50.7 0.4 C24 D:SIK201 4.2 33.9 0.4 C28 D:SIK201 4.2 37.8 0.6 C18 D:SIK201 4.2 43.4 0.6 C19 D:SIK201 4.2 41.5 0.6 C27 D:SIK201 4.3 39.9 0.6 C24 D:SIK201 4.3 50.4 0.6 C23 D:SIK201 4.3 50.2 0.6 C33 D:SIK201 4.3 38.8 0.6 C32 D:SIK201 4.3 26.0 0.6 C14 D:SIK201 4.3 43.8 0.4 C26 D:SIK201 4.4 48.9 0.4 C29 D:SIK201 4.4 57.4 0.4 C33 D:SIK201 4.4 54.2 0.4 H25 D:SIK201 4.5 53.6 0.6 H30 D:SIK201 4.5 35.0 0.6 C25 D:SIK201 4.5 45.0 0.4 C30 D:SIK201 4.5 54.5 0.4 C23 D:SIK201 4.6 37.7 0.4 H22 D:SIK201 4.6 52.5 0.4 H12 D:SIK201 4.8 53.5 0.4 C32 D:SIK201 4.8 51.7 0.4 H27 D:SIK201 5.0 40.6 0.4 N3 D:SIK201 5.0 44.6 0.4 C14 D:SIK201 5.0 35.5 0.6

M.Mukherjee, V.Waser, N.V.Igareta, A.H.Follmer, R.P.Jakob, T.Maier, D.Uzumcu, T.R.Ward. An Artificial Peroxidase Based on the Biotin-Streptavidin Technology That Rivals Natural Peroxidases To Be Published.