Iron in PDB 28lz: Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak

All present enzymatic activity of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak:
1.16.3.1;

The structure of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak, PDB code: 28lz was solved by Z.Bugg, J.M.Bradley, N.E.Le Brun, A.M.Hemmings, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	91.38 / 1.94
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	182.76, 182.76, 182.76, 90, 90, 90
R / Rfree (%)	16.8 / 19.5

The structure of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak also contains other interesting chemical elements:

Chlorine	(Cl)	3 atoms
Magnesium	(Mg)	8 atoms

The binding sites of Iron atom in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak (pdb code 28lz). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak, PDB code: 28lz:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:42.2 occ:1.00 OE1 A:GLU62 2.0 32.2 1.0 OE1 A:GLU27 2.2 31.6 1.0 O A:HOH330 2.2 28.3 1.0 ND1 A:HIS65 2.4 46.9 1.0 O A:HOH303 2.5 43.4 1.0 O A:HOH390 2.6 28.6 1.0 CD A:GLU62 3.1 32.3 1.0 CD A:GLU27 3.1 30.2 1.0 CE1 A:HIS65 3.3 61.8 1.0 FE A:FE203 3.4 45.6 0.9 OE2 A:GLU62 3.4 36.3 1.0 CG A:HIS65 3.4 37.1 1.0 OE2 A:GLU27 3.5 31.9 1.0 OE1 A:GLN141 3.7 41.1 1.0 CB A:HIS65 3.7 22.8 1.0 CG1 A:VAL110 4.4 34.1 1.0 CG A:GLU62 4.4 26.5 1.0 NE2 A:HIS65 4.5 72.0 1.0 CG A:GLU27 4.5 25.0 1.0 CD2 A:HIS65 4.6 45.4 1.0 CA A:GLU62 4.6 25.9 1.0 CB A:GLU62 4.8 25.4 1.0 CD A:GLN141 4.8 32.6 1.0 CB A:GLU27 4.8 23.5 1.0 OE1 A:GLU107 5.0 31.4 1.0

Iron binding site 2 out of 4 in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe202 b:40.5 occ:0.15 CL A:CL206 2.3 30.7 0.2 O A:HOH458 2.4 35.8 0.2 CE1 A:HIS173 2.4 39.9 1.0 NE2 A:HIS173 2.7 42.6 1.0 ND1 A:HIS173 3.7 43.9 1.0 CD2 A:HIS173 4.1 42.4 1.0 CG A:HIS173 4.6 36.4 1.0

Iron binding site 3 out of 4 in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe203 b:45.6 occ:0.90 OE2 A:GLU62 1.9 36.3 1.0 OE1 A:GLU107 2.2 31.4 1.0 OE2 A:GLU107 2.3 41.2 1.0 O A:HOH303 2.3 43.4 1.0 O A:HOH429 2.4 39.7 1.0 O A:HOH390 2.5 28.6 1.0 CD A:GLU107 2.5 37.6 1.0 CD A:GLU62 2.9 32.3 1.0 OE1 A:GLU62 3.3 32.2 1.0 FE A:FE201 3.4 42.2 1.0 OE1 A:GLN141 3.7 41.1 1.0 O A:HOH418 3.8 53.2 1.0 MG A:MG213 4.0 57.6 0.8 CG A:GLU107 4.1 31.7 1.0 O A:HOH373 4.1 46.4 1.0 CG A:GLU62 4.2 26.5 1.0 NE2 A:GLN141 4.3 36.0 1.0 CD A:GLN141 4.4 32.6 1.0 CE2 A:TYR34 4.5 24.9 1.0 OH A:TYR34 4.5 34.8 1.0 O A:HOH330 4.6 28.3 1.0 ND1 A:HIS65 4.7 46.9 1.0 CE1 A:HIS65 4.7 61.8 1.0 CB A:GLU107 4.8 28.2 1.0

Iron binding site 4 out of 4 in the Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Iron Loaded Human H-Chain Ferritin, 20 Minute Oxygen Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe204 b:66.7 occ:0.50 OE2 A:GLU61 1.9 58.1 0.6 O A:HOH311 2.3 70.5 1.0 NE2 A:HIS57 2.4 51.7 1.0 O A:HOH422 2.6 61.6 1.0 O A:HOH411 2.6 73.3 1.0 O A:HOH302 2.6 72.1 1.0 CD A:GLU61 3.1 52.4 0.6 CD2 A:HIS57 3.4 56.1 1.0 CE1 A:HIS57 3.4 51.6 1.0 OE2 A:GLU61 3.5 61.4 0.4 CD A:GLU61 3.6 52.4 0.4 CG A:GLU61 3.9 44.7 0.6 CG A:GLU61 3.9 43.8 0.4 MG A:MG211 3.9 51.3 0.7 O A:HOH427 4.0 57.8 1.0 OE1 A:GLU61 4.0 52.5 0.6 OE1 A:GLU61 4.2 55.5 0.4 ND1 A:HIS57 4.5 58.4 1.0 CG A:HIS57 4.6 48.7 1.0 O A:HOH301 5.0 63.7 1.0

Z.Bugg, J.M.Bradley, A.M.Hemmings, N.E.Le Brun. Ferritin Iron Mineralisation: Route of Fe 3+ Transfer From the Ferroxidase Centre to the Inner Cavity of Human H-Chain Ferritin. Angew.Chem.Int.Ed.Engl. 03843 2026.
ISSN: ESSN 1521-3773
PubMed: 42216818
DOI: 10.1002/ANIE.1203843