Iron in PDB 32fi: Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase

All present enzymatic activity of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase:
1.7.2.5;

The structure of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Iron atom in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase (pdb code 32fi). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase, PDB code: 32fi:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe801 b:93.3 occ:1.00 FE A:HEM801 0.0 93.3 1.0 NC A:HEM801 2.0 81.2 1.0 ND A:HEM801 2.0 75.6 1.0 NA A:HEM801 2.0 79.2 1.0 NB A:HEM801 2.0 83.8 1.0 NE2 A:HIS331 2.1 75.3 1.0 NE2 A:HIS631 2.7 81.8 1.0 CD2 A:HIS331 2.9 74.0 1.0 C4D A:HEM801 3.0 71.5 1.0 C1D A:HEM801 3.1 76.3 1.0 C1C A:HEM801 3.1 73.8 1.0 C1A A:HEM801 3.1 75.8 1.0 C4C A:HEM801 3.1 75.4 1.0 C4B A:HEM801 3.1 79.1 1.0 C4A A:HEM801 3.1 79.1 1.0 C1B A:HEM801 3.1 82.0 1.0 CE1 A:HIS331 3.1 73.2 1.0 CHA A:HEM801 3.4 70.5 1.0 CHC A:HEM801 3.4 70.1 1.0 CHD A:HEM801 3.4 75.1 1.0 CD2 A:HIS631 3.4 79.9 1.0 CHB A:HEM801 3.4 79.4 1.0 CE1 A:HIS631 3.5 84.8 1.0 CG A:HIS331 4.1 78.1 1.0 ND1 A:HIS331 4.2 79.7 1.0 C3D A:HEM801 4.2 75.0 1.0 C2D A:HEM801 4.3 75.7 1.0 C2C A:HEM801 4.3 73.6 1.0 C2A A:HEM801 4.3 78.2 1.0 C3A A:HEM801 4.3 77.7 1.0 C3C A:HEM801 4.3 73.1 1.0 C2B A:HEM801 4.3 86.0 1.0 C3B A:HEM801 4.3 85.3 1.0 CG A:HIS631 4.4 77.8 1.0 ND1 A:HIS631 4.4 83.7 1.0 NE2 A:GLN296 4.8 74.1 1.0 NH2 A:ARG720 5.0 83.1 1.0 CA A:GLY300 5.0 78.2 1.0

Iron binding site 2 out of 4 in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe802 b:114.9 occ:1.00 FE A:HEM802 0.0 114.9 1.0 NE2 A:HIS629 2.0 90.7 1.0 NA A:HEM802 2.0 93.8 1.0 ND A:HEM802 2.0 94.4 1.0 NC A:HEM802 2.0 102.2 1.0 NB A:HEM802 2.0 100.5 1.0 O A:HOH928 2.8 81.6 1.0 CD2 A:HIS629 2.8 88.7 1.0 C4D A:HEM802 3.0 87.6 1.0 C1D A:HEM802 3.0 95.1 1.0 C1A A:HEM802 3.1 88.5 1.0 C4A A:HEM802 3.1 88.3 1.0 C4C A:HEM802 3.1 97.8 1.0 C1B A:HEM802 3.1 94.2 1.0 C1C A:HEM802 3.1 97.5 1.0 C4B A:HEM802 3.1 97.5 1.0 CE1 A:HIS629 3.2 88.4 1.0 CHA A:HEM802 3.4 86.6 1.0 CHD A:HEM802 3.4 94.2 1.0 CHB A:HEM802 3.4 84.7 1.0 CHC A:HEM802 3.4 97.9 1.0 CG A:HIS629 4.0 88.2 1.0 ND1 A:HIS629 4.2 90.6 1.0 C2D A:HEM802 4.2 94.8 1.0 C3D A:HEM802 4.2 84.0 1.0 C3A A:HEM802 4.3 84.3 1.0 C2A A:HEM802 4.3 88.8 1.0 C2B A:HEM802 4.3 91.5 1.0 C2C A:HEM802 4.3 99.4 1.0 C3C A:HEM802 4.3 100.0 1.0 C3B A:HEM802 4.3 90.6 1.0 NE2 A:HIS537 4.7 100.1 1.0

Iron binding site 3 out of 4 in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe801 b:93.7 occ:1.00 FE B:HEM801 0.0 93.7 1.0 NB B:HEM801 2.0 76.7 1.0 ND B:HEM801 2.0 78.1 1.0 NC B:HEM801 2.0 76.8 1.0 NA B:HEM801 2.0 73.0 1.0 NE2 B:HIS331 2.1 78.0 1.0 NE2 B:HIS631 2.7 80.7 1.0 CD2 B:HIS331 2.9 72.2 1.0 C4D B:HEM801 3.1 72.9 1.0 C1D B:HEM801 3.1 69.4 1.0 C1C B:HEM801 3.1 71.9 1.0 C1B B:HEM801 3.1 78.1 1.0 C4B B:HEM801 3.1 74.5 1.0 C4A B:HEM801 3.1 74.1 1.0 C4C B:HEM801 3.1 71.8 1.0 C1A B:HEM801 3.1 71.0 1.0 CE1 B:HIS331 3.2 76.7 1.0 CD2 B:HIS631 3.4 75.3 1.0 CHC B:HEM801 3.4 72.0 1.0 CHD B:HEM801 3.4 68.1 1.0 CHB B:HEM801 3.4 75.2 1.0 CHA B:HEM801 3.4 70.2 1.0 CE1 B:HIS631 3.4 83.9 1.0 CG B:HIS331 4.1 77.6 1.0 ND1 B:HIS331 4.2 80.2 1.0 C3D B:HEM801 4.3 72.4 1.0 C2D B:HEM801 4.3 66.6 1.0 C3A B:HEM801 4.3 69.9 1.0 C2B B:HEM801 4.3 79.8 1.0 C2A B:HEM801 4.3 68.8 1.0 C2C B:HEM801 4.3 70.7 1.0 C3B B:HEM801 4.3 79.0 1.0 C3C B:HEM801 4.3 68.2 1.0 ND1 B:HIS631 4.3 82.2 1.0 CG B:HIS631 4.3 74.5 1.0 NE2 B:GLN296 4.7 79.5 1.0

Iron binding site 4 out of 4 in the Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Cryoem Structure of the E494A Quinol-Dependent Nitric Oxide Reductase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe802 b:114.9 occ:1.00 FE B:HEM802 0.0 114.9 1.0 NA B:HEM802 2.0 89.5 1.0 ND B:HEM802 2.1 88.3 1.0 NB B:HEM802 2.1 93.0 1.0 NC B:HEM802 2.1 100.3 1.0 O B:HOH932 2.1 80.7 1.0 NE2 B:HIS629 2.6 93.2 1.0 C4D B:HEM802 3.0 85.1 1.0 C1D B:HEM802 3.1 90.4 1.0 C1A B:HEM802 3.1 78.4 1.0 C4A B:HEM802 3.1 85.6 1.0 C1B B:HEM802 3.1 88.6 1.0 C4C B:HEM802 3.1 96.2 1.0 C4B B:HEM802 3.1 94.8 1.0 C1C B:HEM802 3.1 101.7 1.0 CD2 B:HIS629 3.1 88.1 1.0 CHA B:HEM802 3.4 84.6 1.0 CHD B:HEM802 3.4 94.3 1.0 CHB B:HEM802 3.4 88.4 1.0 CHC B:HEM802 3.5 99.7 1.0 CE1 B:HIS629 3.9 97.2 1.0 C2D B:HEM802 4.2 88.8 1.0 C3D B:HEM802 4.2 83.9 1.0 C3A B:HEM802 4.3 70.5 1.0 C2A B:HEM802 4.3 70.6 1.0 C2B B:HEM802 4.3 89.2 1.0 C3B B:HEM802 4.3 91.1 1.0 C3C B:HEM802 4.3 99.7 1.0 C2C B:HEM802 4.3 100.8 1.0 CG B:HIS629 4.4 88.0 1.0 NE2 B:HIS537 4.5 86.2 1.0 OE1 B:GLU490 4.6 116.0 1.0 ND1 B:HIS629 4.8 97.8 1.0 NE2 B:HIS538 4.8 89.5 1.0 CE1 B:HIS537 4.8 93.2 1.0 CE1 B:HIS538 4.9 86.0 1.0

F.Khaja, S.V.Antonyuk, S.P.Muench, S.S.Hsanain. Cryo-Em Structure of the N600A Quinol-Dependent Nitric Oxide Reductase To Be Published.