Iron in PDB 1hbr: R-State Form of Chicken Hemoglobin D

The structure of R-State Form of Chicken Hemoglobin D, PDB code: 1hbr was solved by E.Knapp, M.A.Oliveira, Q.Xie, S.R.Ernst, A.F.Riggs, M.L.Hackert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.960, 80.510, 82.110, 90.00, 104.50, 90.00
R / Rfree (%)	22.2 / 25.7

The binding sites of Iron atom in the R-State Form of Chicken Hemoglobin D (pdb code 1hbr). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the R-State Form of Chicken Hemoglobin D, PDB code: 1hbr:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the R-State Form of Chicken Hemoglobin D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of R-State Form of Chicken Hemoglobin D within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe142 b:20.2 occ:1.00 FE A:HEM142 0.0 20.2 1.0 O A:HOH143 1.7 22.0 1.0 NC A:HEM142 1.9 7.5 1.0 ND A:HEM142 1.9 19.2 1.0 NA A:HEM142 2.0 23.4 1.0 NB A:HEM142 2.0 26.2 1.0 NE2 A:HIS87 2.1 15.6 1.0 O A:HOH144 2.9 31.9 1.0 C4D A:HEM142 3.0 26.2 1.0 C4C A:HEM142 3.0 14.7 1.0 C1C A:HEM142 3.0 21.2 1.0 CE1 A:HIS87 3.0 3.1 1.0 C1A A:HEM142 3.0 26.0 1.0 C1D A:HEM142 3.0 7.3 1.0 C4A A:HEM142 3.0 23.4 1.0 C4B A:HEM142 3.0 25.8 1.0 C1B A:HEM142 3.1 16.8 1.0 CD2 A:HIS87 3.1 7.2 1.0 CHA A:HEM142 3.4 7.2 1.0 CHC A:HEM142 3.4 14.7 1.0 CHD A:HEM142 3.4 13.1 1.0 CHB A:HEM142 3.4 35.3 1.0 ND1 A:HIS87 4.1 8.8 1.0 C3D A:HEM142 4.2 10.8 1.0 C3C A:HEM142 4.2 6.9 1.0 CG A:HIS87 4.2 14.3 1.0 C2C A:HEM142 4.2 10.1 1.0 C2A A:HEM142 4.3 21.0 1.0 C3A A:HEM142 4.3 20.9 1.0 C2D A:HEM142 4.3 10.4 1.0 C2B A:HEM142 4.3 35.9 1.0 C3B A:HEM142 4.3 32.3 1.0 NE2 A:HIS58 4.3 36.6 1.0 CE1 A:HIS58 4.7 13.6 1.0

Iron binding site 2 out of 4 in the R-State Form of Chicken Hemoglobin D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of R-State Form of Chicken Hemoglobin D within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe147 b:22.5 occ:1.00 FE B:HEM147 0.0 22.5 1.0 O B:HOH148 1.8 24.4 1.0 NC B:HEM147 2.0 11.5 1.0 NA B:HEM147 2.0 9.8 1.0 ND B:HEM147 2.0 30.0 1.0 NB B:HEM147 2.0 26.4 1.0 NE2 B:HIS92 2.0 16.4 1.0 CE1 B:HIS92 3.0 9.6 1.0 C4D B:HEM147 3.0 12.9 1.0 O B:HOH149 3.0 37.0 1.0 C4C B:HEM147 3.0 23.4 1.0 C1A B:HEM147 3.0 29.1 1.0 C1C B:HEM147 3.0 17.3 1.0 C4A B:HEM147 3.0 27.8 1.0 CD2 B:HIS92 3.0 12.5 1.0 C1D B:HEM147 3.0 12.2 1.0 C4B B:HEM147 3.0 12.7 1.0 C1B B:HEM147 3.0 19.0 1.0 CHA B:HEM147 3.4 7.0 1.0 CHD B:HEM147 3.4 23.1 1.0 CHC B:HEM147 3.4 24.8 1.0 CHB B:HEM147 3.4 23.2 1.0 ND1 B:HIS92 4.1 15.5 1.0 CG B:HIS92 4.1 11.6 1.0 C3D B:HEM147 4.2 14.3 1.0 C3A B:HEM147 4.2 16.4 1.0 C2A B:HEM147 4.2 35.0 1.0 C3C B:HEM147 4.2 18.6 1.0 C2C B:HEM147 4.3 11.6 1.0 C2B B:HEM147 4.3 10.5 1.0 C2D B:HEM147 4.3 17.7 1.0 C3B B:HEM147 4.3 25.1 1.0 NE2 B:HIS63 4.5 30.5 1.0 CG2 B:VAL67 4.9 10.4 1.0 CE1 B:HIS63 5.0 10.6 1.0

Iron binding site 3 out of 4 in the R-State Form of Chicken Hemoglobin D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of R-State Form of Chicken Hemoglobin D within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe142 b:20.0 occ:1.00 FE C:HEM142 0.0 20.0 1.0 O C:HOH145 1.8 21.5 1.0 NC C:HEM142 1.9 9.4 1.0 ND C:HEM142 1.9 19.4 1.0 NA C:HEM142 2.0 20.1 1.0 NB C:HEM142 2.0 27.6 1.0 NE2 C:HIS87 2.1 18.0 1.0 C4D C:HEM142 3.0 23.3 1.0 C1C C:HEM142 3.0 20.8 1.0 C4C C:HEM142 3.0 12.9 1.0 C1A C:HEM142 3.0 24.8 1.0 CE1 C:HIS87 3.0 2.3 1.0 C1D C:HEM142 3.0 7.5 1.0 C4A C:HEM142 3.0 24.1 1.0 C4B C:HEM142 3.0 24.9 1.0 C1B C:HEM142 3.1 14.7 1.0 CD2 C:HIS87 3.1 7.0 1.0 CHA C:HEM142 3.4 8.2 1.0 CHC C:HEM142 3.4 15.8 1.0 CHD C:HEM142 3.4 13.3 1.0 CHB C:HEM142 3.4 34.4 1.0 ND1 C:HIS87 4.1 7.4 1.0 C3D C:HEM142 4.2 11.5 1.0 C3C C:HEM142 4.2 4.3 1.0 CG C:HIS87 4.2 14.6 1.0 C2C C:HEM142 4.2 10.5 1.0 C2A C:HEM142 4.3 22.6 1.0 C3A C:HEM142 4.3 21.2 1.0 C2D C:HEM142 4.3 11.9 1.0 C2B C:HEM142 4.3 36.0 1.0 C3B C:HEM142 4.3 32.2 1.0 NE2 C:HIS58 4.3 35.6 1.0 CE1 C:HIS58 4.7 12.5 1.0

Iron binding site 4 out of 4 in the R-State Form of Chicken Hemoglobin D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of R-State Form of Chicken Hemoglobin D within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe147 b:21.3 occ:1.00 FE D:HEM147 0.0 21.3 1.0 O D:HOH148 1.8 22.9 1.0 NE2 D:HIS92 1.9 18.3 1.0 ND D:HEM147 2.0 26.3 1.0 NC D:HEM147 2.0 14.1 1.0 NA D:HEM147 2.0 6.0 1.0 NB D:HEM147 2.0 26.0 1.0 O D:HOH149 2.9 41.4 1.0 CE1 D:HIS92 2.9 9.5 1.0 CD2 D:HIS92 3.0 11.9 1.0 C4D D:HEM147 3.0 13.7 1.0 C4C D:HEM147 3.0 24.9 1.0 C1A D:HEM147 3.0 27.0 1.0 C1C D:HEM147 3.0 16.3 1.0 C4A D:HEM147 3.0 26.4 1.0 C1D D:HEM147 3.0 13.0 1.0 C4B D:HEM147 3.0 12.2 1.0 C1B D:HEM147 3.1 21.0 1.0 CHA D:HEM147 3.4 10.3 1.0 CHD D:HEM147 3.4 25.2 1.0 CHC D:HEM147 3.4 26.9 1.0 CHB D:HEM147 3.4 18.9 1.0 ND1 D:HIS92 4.0 13.1 1.0 CG D:HIS92 4.1 10.9 1.0 C3D D:HEM147 4.2 13.7 1.0 C3A D:HEM147 4.2 16.7 1.0 C2A D:HEM147 4.2 33.8 1.0 C3C D:HEM147 4.2 19.7 1.0 C2C D:HEM147 4.3 11.2 1.0 C2D D:HEM147 4.3 16.8 1.0 C2B D:HEM147 4.3 11.0 1.0 C3B D:HEM147 4.3 26.0 1.0 NE2 D:HIS63 4.6 31.2 1.0 CG2 D:VAL67 5.0 12.9 1.0

J.E.Knapp, M.A.Oliveira, Q.Xie, S.R.Ernst, A.F.Riggs, M.L.Hackert. The Structural and Functional Analysis of the Hemoglobin D Component From Chicken. J.Biol.Chem. V. 274 6411 1999.
ISSN: ISSN 0021-9258
PubMed: 10037733
DOI: 10.1074/JBC.274.10.6411