Iron in PDB 1r9x: Bacterial Cytosine Deaminase D314G Mutant.

All present enzymatic activity of Bacterial Cytosine Deaminase D314G Mutant.:
3.5.4.1;

The structure of Bacterial Cytosine Deaminase D314G Mutant., PDB code: 1r9x was solved by S.D.Mahan, G.C.Ireton, B.L.Stoddard, M.E.Black, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 1.58
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	109.037, 109.037, 240.188, 90.00, 90.00, 120.00
R / Rfree (%)	16.4 / 18.4

The structure of Bacterial Cytosine Deaminase D314G Mutant. also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Iron atom in the Bacterial Cytosine Deaminase D314G Mutant. (pdb code 1r9x). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Bacterial Cytosine Deaminase D314G Mutant., PDB code: 1r9x:

Iron binding site 1 out of 1 in the Bacterial Cytosine Deaminase D314G Mutant.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Bacterial Cytosine Deaminase D314G Mutant. within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:14.6 occ:1.00 O A:HOH600 1.8 15.6 1.0 NE2 A:HIS63 2.1 10.1 1.0 NE2 A:HIS61 2.1 7.5 1.0 NE2 A:HIS214 2.2 9.8 1.0 CE1 A:HIS61 3.0 9.4 1.0 OD2 A:ASP313 3.0 16.2 1.0 CE1 A:HIS63 3.0 11.4 1.0 CD2 A:HIS63 3.0 10.7 1.0 CE1 A:HIS214 3.1 8.8 1.0 CD2 A:HIS61 3.2 8.1 1.0 CD2 A:HIS214 3.2 8.9 1.0 MG A:MG502 3.5 17.9 1.0 O A:HOH605 3.7 24.9 1.0 NE2 A:HIS246 3.7 9.3 1.0 CG A:ASP313 3.8 13.5 1.0 O A:HOH601 3.9 20.5 1.0 OD1 A:ASP313 4.0 13.7 1.0 ND1 A:HIS61 4.1 7.8 1.0 ND1 A:HIS63 4.1 9.7 1.0 CG A:HIS63 4.2 8.9 1.0 ND1 A:HIS214 4.2 9.0 1.0 CG A:HIS61 4.3 6.9 1.0 CG A:HIS214 4.3 8.2 1.0 O A:HOH602 4.3 17.8 1.0 O A:HOH603 4.4 19.9 1.0 CD2 A:HIS246 4.5 9.5 1.0 CE1 A:HIS246 4.7 11.4 1.0 CD2 A:HIS122 4.7 8.4 1.0 CG A:HIS122 4.9 7.6 1.0

S.D.Mahan, G.C.Ireton, C.Knoeber, B.L.Stoddard, M.E.Black. Random Mutagenesis and Selection of Escherichia Coli Cytosine Deaminase For Cancer Gene Therapy. Protein Eng.Des.Sel. V. 17 625 2004.
ISSN: ISSN 1741-0126
PubMed: 15381761
DOI: 10.1093/PROTEIN/GZH074