Iron in PDB 1rfk: Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus

The structure of Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus, PDB code: 1rfk was solved by A.Fish, R.Nechushtai, O.Livnah, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.25
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	28.450, 50.934, 110.913, 90.00, 90.00, 90.00
R / Rfree (%)	21.1 / 24.7

The binding sites of Iron atom in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus (pdb code 1rfk). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus, PDB code: 1rfk:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe100 b:8.6 occ:1.00 FE1 A:FES100 0.0 8.6 1.0 S2 A:FES100 2.2 8.9 1.0 S1 A:FES100 2.2 8.1 1.0 SG A:CYS46 2.3 9.8 1.0 SG A:CYS41 2.3 9.1 1.0 FE2 A:FES100 2.7 7.4 1.0 CB A:CYS46 3.4 12.5 1.0 N A:CYS41 3.6 8.5 1.0 CB A:CYS41 3.6 9.2 1.0 N A:CYS46 3.6 10.0 1.0 N A:ARG42 3.8 7.8 1.0 CA A:CYS46 3.8 8.6 1.0 CA A:CYS41 4.0 8.0 1.0 C A:CYS46 4.0 9.6 1.0 O A:CYS46 4.1 12.2 1.0 OG A:SER40 4.2 10.6 0.5 N A:ALA45 4.3 9.0 1.0 C A:CYS41 4.3 9.9 1.0 N A:GLY44 4.4 10.0 1.0 SG A:CYS79 4.4 7.0 1.0 N A:ALA43 4.5 8.4 1.0 C A:ALA45 4.5 9.5 1.0 SG A:CYS49 4.5 7.7 1.0 C A:SER40 4.6 7.9 1.0 N A:THR48 4.6 7.8 1.0 CA A:ARG42 4.7 6.2 1.0 N A:SER40 4.7 8.9 1.0 N A:SER47 4.7 10.9 1.0 C A:GLY44 4.9 11.2 1.0 CA A:GLY44 4.9 8.2 1.0 CA A:ALA45 5.0 9.9 1.0 CB A:THR48 5.0 9.5 1.0

Iron binding site 2 out of 4 in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe100 b:7.4 occ:1.00 FE2 A:FES100 0.0 7.4 1.0 S2 A:FES100 2.2 8.9 1.0 S1 A:FES100 2.2 8.1 1.0 SG A:CYS49 2.2 7.7 1.0 SG A:CYS79 2.3 7.0 1.0 FE1 A:FES100 2.7 8.6 1.0 CB A:CYS79 3.3 9.4 1.0 CB A:CYS49 3.3 10.2 1.0 N A:CYS79 4.2 6.4 1.0 N A:CYS49 4.3 7.3 1.0 N A:GLY44 4.3 10.0 1.0 CA A:CYS79 4.3 7.8 1.0 N A:ARG42 4.4 7.8 1.0 CA A:CYS49 4.4 7.9 1.0 SG A:CYS41 4.4 9.1 1.0 CA A:ARG42 4.4 6.2 1.0 CB A:LEU77 4.5 7.5 1.0 SG A:CYS46 4.6 9.8 1.0 N A:ALA43 4.6 8.4 1.0 CA A:GLY44 4.7 8.2 1.0 O A:CYS46 4.8 12.2 1.0 C A:ARG42 4.9 6.5 1.0

Iron binding site 3 out of 4 in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe300 b:11.0 occ:1.00 FE1 B:FES300 0.0 11.0 1.0 S2 B:FES300 2.2 10.5 1.0 S1 B:FES300 2.3 10.9 1.0 SG B:CYS246 2.3 12.1 1.0 SG B:CYS241 2.3 10.7 1.0 FE2 B:FES300 2.7 10.1 1.0 CB B:CYS246 3.4 15.6 1.0 CB B:CYS241 3.4 10.7 1.0 N B:CYS241 3.5 10.4 1.0 N B:CYS246 3.5 14.2 1.0 N B:ARG242 3.8 12.5 1.0 CA B:CYS246 3.8 13.2 1.0 CA B:CYS241 3.9 13.1 1.0 C B:CYS246 4.1 13.9 1.0 O B:CYS246 4.1 14.9 1.0 OG B:SER240 4.2 15.0 0.3 N B:ALA245 4.2 12.8 1.0 C B:CYS241 4.3 13.3 1.0 SG B:CYS279 4.4 10.2 1.0 N B:GLY244 4.4 10.2 1.0 C B:ALA245 4.5 15.4 1.0 N B:ALA243 4.5 11.6 1.0 C B:SER240 4.6 12.3 1.0 N B:THR248 4.6 12.6 1.0 SG B:CYS249 4.6 10.7 1.0 CA B:ARG242 4.6 12.1 1.0 N B:SER240 4.6 12.5 1.0 N B:SER247 4.8 13.1 1.0 CA B:GLY244 4.8 9.0 1.0 C B:GLY244 4.9 10.2 1.0 CA B:ALA245 4.9 10.0 1.0 CB B:SER240 4.9 14.5 1.0 CB B:THR248 4.9 13.2 1.0 CA B:SER240 5.0 12.9 1.0

Iron binding site 4 out of 4 in the Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of 2FE2S Ferredoxin From Thermophilic Cyanobacterium Mastigocladus Laminosus within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe300 b:10.1 occ:1.00 FE2 B:FES300 0.0 10.1 1.0 S2 B:FES300 2.2 10.5 1.0 S1 B:FES300 2.2 10.9 1.0 SG B:CYS249 2.2 10.7 1.0 SG B:CYS279 2.3 10.2 1.0 FE1 B:FES300 2.7 11.0 1.0 CB B:CYS249 3.2 10.8 1.0 CB B:CYS279 3.3 11.8 1.0 N B:CYS279 4.2 9.8 1.0 N B:CYS249 4.3 12.9 1.0 N B:GLY244 4.3 10.2 1.0 CA B:CYS249 4.3 11.9 1.0 CA B:CYS279 4.4 11.4 1.0 SG B:CYS241 4.4 10.7 1.0 N B:ARG242 4.4 12.5 1.0 CA B:ARG242 4.4 12.1 1.0 CB B:LEU277 4.5 9.5 1.0 CA B:GLY244 4.6 9.0 1.0 SG B:CYS246 4.6 12.1 1.0 N B:ALA243 4.7 11.6 1.0 C B:ARG242 4.9 10.0 1.0 O B:CYS246 4.9 14.9 1.0

A.Fish, T.Danieli, I.Ohad, R.Nechushtai, O.Livnah. Structural Basis For the Thermostability of Ferredoxin From the Cyanobacterium Mastigocladus Laminosus. J.Mol.Biol. V. 350 599 2005.
ISSN: ISSN 0022-2836
PubMed: 15961101
DOI: 10.1016/J.JMB.2005.04.071