Iron in PDB 2b5h: 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase

All present enzymatic activity of 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase:
1.13.11.20;

The structure of 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase, PDB code: 2b5h was solved by C.R.Simmons, Q.Liu, Q.Huang, Q.Hao, T.P.Begley, P.A.Karplus, M.H.Stipanuk, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.78 / 1.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	57.553, 57.553, 123.070, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 20.8

The binding sites of Iron atom in the 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase (pdb code 2b5h). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase, PDB code: 2b5h:

Iron binding site 1 out of 1 in the 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of 1.5 A Resolution Crystal Structure of Recombinant R. Norvegicus Cysteine Dioxygenase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:11.7 occ:1.00 NE2 A:HIS140 2.1 11.0 1.0 NE2 A:HIS88 2.1 11.2 1.0 NE2 A:HIS86 2.1 13.8 1.0 O A:HOH505 2.1 9.9 1.0 CE1 A:HIS88 2.9 14.5 1.0 CD2 A:HIS140 3.0 12.3 1.0 CD2 A:HIS86 3.0 13.9 1.0 CE1 A:HIS140 3.1 10.7 1.0 CE1 A:HIS86 3.1 13.0 1.0 CD2 A:HIS88 3.2 9.8 1.0 O A:HOH628 3.7 31.2 1.0 ND1 A:HIS88 4.1 11.2 1.0 CG A:HIS86 4.2 14.1 1.0 OH A:TYR157 4.2 22.1 1.0 CG A:HIS140 4.2 11.3 1.0 ND1 A:HIS86 4.2 11.3 1.0 ND1 A:HIS140 4.2 12.0 1.0 SG A:CYS93 4.2 18.3 1.0 CG A:HIS88 4.3 10.6 1.0 O A:HOH656 4.6 32.8 1.0 CG2 A:ILE133 4.7 12.3 1.0 CG2 A:VAL142 4.8 14.0 1.0 O A:HOH651 4.8 32.0 1.0 O A:HOH629 4.8 27.0 1.0

C.R.Simmons, Q.Liu, Q.Huang, Q.Hao, T.P.Begley, P.A.Karplus, M.H.Stipanuk. Crystal Structure of Mammalian Cysteine Dioxygenase: A Novel Mononuclear Iron Center For Cysteine Thiol Oxidation. J.Biol.Chem. V. 281 18723 2006.
ISSN: ISSN 0021-9258
PubMed: 16611640
DOI: 10.1074/JBC.M601555200