Atomistry » Iron » PDB 2grz-2hk6 » 2hk6
Atomistry »
  Iron »
    PDB 2grz-2hk6 »
      2hk6 »

Iron in PDB 2hk6: Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site

Enzymatic activity of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site

All present enzymatic activity of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site:
4.99.1.1;

Protein crystallography data

The structure of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site, PDB code: 2hk6 was solved by S.Al-Karadaghi, T.Karlberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.64 / 1.71
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 48.650, 49.980, 118.850, 90.00, 90.00, 90.00
R / Rfree (%) 17.6 / 22

Other elements in 2hk6:

The structure of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site (pdb code 2hk6). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site, PDB code: 2hk6:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in 2hk6

Go back to Iron Binding Sites List in 2hk6
Iron binding site 1 out of 4 in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe500

b:16.5
occ:0.87
 O A:HOH926 1.8 28.5 1.0
O A:HOH674 2.1 22.1 1.0
NE2 A:HIS183 2.2 13.1 1.0
OE2 A:GLU264 2.2 23.2 1.0
O A:HOH675 2.3 17.1 1.0
CE1 A:HIS183 3.1 13.6 1.0
CD2 A:HIS183 3.2 14.0 1.0
CD A:GLU264 3.2 21.2 1.0
OE1 A:GLU264 3.5 23.2 1.0
OH A:TYR13 3.9 20.3 1.0
O A:SER222 4.0 14.9 1.0
CZ A:TYR13 4.2 19.1 1.0
ND1 A:HIS183 4.2 13.2 1.0
O A:HOH794 4.3 26.4 1.0
CG A:HIS183 4.3 11.9 1.0
CG A:GLU264 4.5 17.8 1.0
CE1 A:TYR13 4.7 18.7 1.0
CB A:SER222 4.7 12.8 1.0
CE2 A:TYR13 4.8 19.3 1.0

Iron binding site 2 out of 4 in 2hk6

Go back to Iron Binding Sites List in 2hk6
Iron binding site 2 out of 4 in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe501

b:15.0
occ:0.63
 O A:HOH745 1.5 24.7 1.0
O A:HOH750 1.9 25.8 1.0
O A:HOH748 2.1 30.7 1.0
NE2 A:HIS201 2.1 14.4 1.0
O A:HOH747 2.3 23.6 1.0
O A:HOH746 2.3 31.4 1.0
CD2 A:HIS201 3.0 14.5 1.0
CE1 A:HIS201 3.2 15.5 1.0
O A:HOH837 4.1 29.6 1.0
O A:HOH749 4.1 27.2 1.0
CG A:HIS201 4.2 12.9 1.0
ND1 A:HIS201 4.3 14.4 1.0
O A:HOH773 4.3 25.2 1.0
O A:HOH880 4.4 34.3 1.0
O A:HOH654 4.4 18.5 1.0
CB A:VAL218 4.5 10.6 1.0
O A:HOH796 4.6 22.5 1.0
CG1 A:VAL218 4.9 11.5 1.0

Iron binding site 3 out of 4 in 2hk6

Go back to Iron Binding Sites List in 2hk6
Iron binding site 3 out of 4 in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe502

b:15.7
occ:0.55
 OE1 A:GLU73 2.1 20.2 1.0
O A:HOH865 2.2 30.0 1.0
O A:HOH743 2.2 24.7 1.0
ND1 A:HIS70 2.3 12.4 1.0
O A:HOH866 2.3 25.0 1.0
O A:HOH742 2.4 30.5 1.0
CE1 A:HIS70 3.1 12.1 1.0
CD A:GLU73 3.1 17.7 1.0
CG A:HIS70 3.4 10.3 1.0
OE2 A:GLU73 3.4 22.5 1.0
CB A:HIS70 3.8 9.4 1.0
CA A:HIS70 4.1 9.2 1.0
O A:HOH688 4.2 20.9 1.0
NE2 A:HIS70 4.3 10.0 1.0
O A:HOH658 4.3 13.8 1.0
O A:HOH709 4.3 19.8 1.0
O A:HOH949 4.4 46.0 1.0
CD2 A:HIS70 4.4 11.2 1.0
CG A:GLU73 4.5 16.5 1.0
CB A:GLU73 4.8 13.9 1.0
N A:HIS70 5.0 8.7 1.0

Iron binding site 4 out of 4 in 2hk6

Go back to Iron Binding Sites List in 2hk6
Iron binding site 4 out of 4 in the Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of B. Subtilis Ferrochelatase with Iron Bound at the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe503

b:16.3
occ:0.37
 OE1 A:GLN69 2.0 11.5 1.0
O A:HOH744 2.1 20.8 1.0
O A:HOH733 2.2 18.5 1.0
O A:HOH752 2.3 46.9 1.0
O A:HOH751 2.6 36.4 1.0
NE2 A:HIS65 2.6 24.0 1.0
CD A:GLN69 3.1 13.9 1.0
CE1 A:HIS65 3.4 23.8 1.0
CD2 A:HIS65 3.6 21.4 1.0
NE2 A:GLN69 3.7 14.4 1.0
O A:HOH824 4.0 15.0 1.0
CG A:GLN69 4.3 9.8 1.0
CB A:GLN69 4.4 8.6 1.0
OD1 A:ASN66 4.5 11.3 1.0
ND1 A:HIS65 4.6 25.2 1.0
CG A:HIS65 4.7 17.2 1.0
O A:HOH658 4.9 13.8 1.0

Reference:

M.D.Hansson, T.Karlberg, M.A.Rahardja, S.Al-Karadaghi, M.Hansson. Amino Acid Residues HIS183 and GLU264 in Bacillus Subtilis Ferrochelatase Direct and Facilitate the Insertion of Metal Ion Into Protoporphyrin IX Biochemistry V. 46 87 2007.
ISSN: ISSN 0006-2960
PubMed: 17198378
DOI: 10.1021/BI061760A
Page generated: Thu Jul 17 02:04:12 2025

Last articles

Zn in 9UUO
Zn in 9UUS
Zn in 9W4R
Zn in 9VKW
Zn in 9W4S
Zn in 9VH1
Zn in 9RMX
Zn in 9RMU
Zn in 9QWN
Zn in 9U9Y
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy