Iron in PDB 2pah: Tetrameric Human Phenylalanine Hydroxylase

All present enzymatic activity of Tetrameric Human Phenylalanine Hydroxylase:
1.14.16.1;

The structure of Tetrameric Human Phenylalanine Hydroxylase, PDB code: 2pah was solved by R.C.Stevens, F.Fusetti, H.Erlandsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.10
Space group	P 31 1 2
Cell size a, b, c (Å), α, β, γ (°)	119.500, 119.500, 126.000, 90.00, 90.00, 120.00
R / Rfree (%)	25.1 / 32.6

The binding sites of Iron atom in the Tetrameric Human Phenylalanine Hydroxylase (pdb code 2pah). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Tetrameric Human Phenylalanine Hydroxylase, PDB code: 2pah:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Tetrameric Human Phenylalanine Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Tetrameric Human Phenylalanine Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe453 b:84.3 occ:1.00 NE2 A:HIS285 2.0 99.5 1.0 NE2 A:HIS290 2.1 54.5 1.0 OE1 A:GLU330 2.1 0.0 1.0 CE1 A:HIS285 2.9 0.0 1.0 CD A:GLU330 2.9 99.9 1.0 CD2 A:HIS290 3.0 49.6 1.0 CE1 A:HIS290 3.1 64.5 1.0 CD2 A:HIS285 3.1 87.2 1.0 OE2 A:GLU330 3.1 81.0 1.0 OE2 A:GLU286 4.0 88.2 1.0 ND1 A:HIS285 4.1 93.0 1.0 ND1 A:HIS290 4.1 64.9 1.0 CG A:HIS290 4.1 62.5 1.0 CG A:HIS285 4.2 76.6 1.0 CG A:GLU330 4.3 97.6 1.0 CG A:PRO281 4.6 70.6 1.0 CB A:ALA345 4.7 88.1 1.0 OH A:TYR325 4.7 0.0 1.0 CB A:PRO281 4.9 51.2 1.0

Iron binding site 2 out of 2 in the Tetrameric Human Phenylalanine Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Tetrameric Human Phenylalanine Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe454 b:91.3 occ:1.00 OE1 B:GLU330 2.0 0.0 1.0 NE2 B:HIS290 2.2 78.0 1.0 NE2 B:HIS285 2.4 94.5 1.0 CD B:GLU330 3.0 0.0 1.0 CE1 B:HIS290 3.0 74.9 1.0 CD2 B:HIS290 3.2 69.2 1.0 CE1 B:HIS285 3.3 92.2 1.0 OE2 B:GLU330 3.3 0.0 1.0 CD2 B:HIS285 3.4 82.0 1.0 OE2 B:GLU286 4.0 87.7 1.0 ND1 B:HIS290 4.1 73.0 1.0 CG B:HIS290 4.2 67.5 1.0 CG B:GLU330 4.3 0.0 1.0 OH B:TYR325 4.4 0.0 1.0 ND1 B:HIS285 4.5 86.2 1.0 CG B:HIS285 4.5 80.9 1.0 CG B:PRO281 4.6 95.3 1.0 CE2 B:PHE254 4.8 0.0 1.0 CE1 B:TYR325 4.8 0.0 1.0 CB B:PRO281 4.9 86.0 1.0 CB B:ALA345 4.9 80.9 1.0 CZ B:PHE254 5.0 0.0 1.0

F.Fusetti, H.Erlandsen, T.Flatmark, R.C.Stevens. Structure of Tetrameric Human Phenylalanine Hydroxylase and Its Implications For Phenylketonuria. J.Biol.Chem. V. 273 16962 1998.
ISSN: ISSN 0021-9258
PubMed: 9642259
DOI: 10.1074/JBC.273.27.16962