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Iron in PDB 3c25: Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna

Protein crystallography data

The structure of Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna, PDB code: 3c25 was solved by A.R.Lambert, D.Sussman, B.Shen, B.L.Stoddard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.50
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 73.890, 81.712, 73.583, 90.00, 99.51, 90.00
R / Rfree (%) 22.3 / 27.7

Other elements in 3c25:

The structure of Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna also contains other interesting chemical elements:

Calcium (Ca) 4 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna (pdb code 3c25). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna, PDB code: 3c25:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 3c25

Go back to Iron Binding Sites List in 3c25
Iron binding site 1 out of 2 in the Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe901

b:64.9
occ:1.00
SG A:CYS55 2.4 62.5 1.0
SG A:CYS81 2.4 62.8 1.0
SG A:CYS65 2.4 61.9 1.0
SG A:CYS42 2.4 62.0 1.0
CB A:CYS81 3.5 62.7 1.0
CB A:CYS65 4.1 61.7 1.0
CB A:CYS55 4.2 63.3 1.0
CB A:CYS42 4.3 62.1 1.0
CA A:CYS65 4.6 61.6 1.0
CB A:ASN83 4.7 62.1 1.0
CE A:LYS57 4.8 60.0 1.0
CA A:CYS55 4.9 63.0 1.0
CD A:LYS57 4.9 60.8 1.0
ND2 A:ASN83 5.0 61.4 1.0

Iron binding site 2 out of 2 in 3c25

Go back to Iron Binding Sites List in 3c25
Iron binding site 2 out of 2 in the Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Noti Restriction Endonuclease Bound to Cognate Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe902

b:64.8
occ:1.00
SG B:CYS55 2.4 62.9 1.0
SG B:CYS42 2.4 62.8 1.0
SG B:CYS65 2.4 62.5 1.0
SG B:CYS81 2.4 63.4 1.0
CB B:CYS81 3.7 62.7 1.0
CB B:CYS65 4.0 62.2 1.0
CB B:CYS55 4.2 63.2 1.0
CB B:CYS42 4.2 62.6 1.0
CA B:CYS65 4.5 62.1 1.0
CE B:LYS57 4.6 59.0 1.0
CB B:ASN83 4.7 62.3 1.0
CG B:LYS57 4.7 61.2 1.0
CD B:LYS57 4.8 59.9 1.0
CA B:CYS55 4.9 63.2 1.0

Reference:

A.R.Lambert, D.Sussman, B.Shen, R.Maunus, J.Nix, J.Samuelson, S.Y.Xu, B.L.Stoddard. Structures of the Rare-Cutting Restriction Endonuclease Noti Reveal A Unique Metal Binding Fold Involved in Dna Binding. Structure V. 16 558 2008.
ISSN: ISSN 0969-2126
PubMed: 18400177
DOI: 10.1016/J.STR.2008.01.017
Page generated: Sun Aug 4 08:15:11 2024

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