Iron in PDB 3hf1: Crystal Structure of Human P53R2

All present enzymatic activity of Crystal Structure of Human P53R2:
1.17.4.1;

The structure of Crystal Structure of Human P53R2, PDB code: 3hf1 was solved by P.Smith, B.Zhou, Y.-C.Yuan, L.Su, S.-C.Tsai, Y.Yen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	68.675, 99.546, 132.190, 90.00, 90.00, 90.00
R / Rfree (%)	22.1 / 27.3

The binding sites of Iron atom in the Crystal Structure of Human P53R2 (pdb code 3hf1). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Crystal Structure of Human P53R2, PDB code: 3hf1:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Crystal Structure of Human P53R2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Human P53R2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe401 b:62.7 occ:1.00 OE1 A:GLU194 2.2 71.7 1.0 ND1 A:HIS231 2.3 39.9 1.0 OE2 A:GLU228 2.4 51.8 1.0 OE2 A:GLU131 2.5 54.5 1.0 OE1 A:GLU131 3.0 52.6 1.0 CE1 A:HIS231 3.0 42.3 1.0 CD A:GLU131 3.1 52.6 1.0 CD A:GLU194 3.1 69.3 1.0 OE2 A:GLU194 3.2 71.9 1.0 CD A:GLU228 3.3 50.5 1.0 CG A:HIS231 3.3 37.6 1.0 OE1 A:GLN127 3.7 44.4 1.0 OE1 A:GLU228 3.7 55.8 1.0 CB A:HIS231 3.8 32.7 1.0 NE2 A:HIS231 4.2 40.4 1.0 CD2 A:HIS231 4.3 39.1 1.0 CG A:GLN127 4.3 42.3 1.0 CA A:GLU228 4.4 36.3 1.0 CD A:GLN127 4.4 44.8 1.0 CE1 A:HIS134 4.5 32.0 1.0 CG A:GLU131 4.5 50.0 1.0 CG A:GLU194 4.5 66.3 1.0 CG A:GLU228 4.5 46.0 1.0 O A:ASP227 4.6 41.0 1.0 ND1 A:HIS134 4.8 32.0 1.0 N A:GLU228 4.8 35.9 1.0 CB A:GLU228 4.9 39.1 1.0 C A:ASP227 4.9 37.2 1.0

Iron binding site 2 out of 3 in the Crystal Structure of Human P53R2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Human P53R2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe401 b:80.0 occ:1.00 OE1 B:GLU194 2.2 81.6 1.0 ND1 B:HIS231 2.4 45.8 1.0 OE1 B:GLU131 2.5 59.1 1.0 OE2 B:GLU228 2.5 55.3 1.0 OE1 B:GLU228 2.7 55.3 1.0 CD B:GLU228 2.8 52.8 1.0 CD B:GLU194 3.1 80.2 1.0 OE2 B:GLU194 3.2 82.3 1.0 CD B:GLU131 3.2 57.5 1.0 OE2 B:GLU131 3.2 60.3 1.0 CE1 B:HIS231 3.3 45.1 1.0 CG B:HIS231 3.4 45.4 1.0 CB B:HIS231 3.7 45.2 1.0 CA B:GLU228 3.8 45.0 1.0 CG B:GLU228 4.0 50.5 1.0 OE1 B:GLN127 4.1 51.4 1.0 CB B:GLU228 4.3 45.7 1.0 O B:HOH354 4.3 67.5 1.0 N B:GLU228 4.4 44.1 1.0 NE2 B:HIS231 4.4 45.1 1.0 CG B:GLU194 4.5 79.0 1.0 CD2 B:HIS231 4.5 46.5 1.0 CG B:GLU131 4.6 55.3 1.0 CE1 B:HIS134 4.7 38.4 1.0 CD B:GLN127 4.8 48.7 1.0 CG B:GLN127 4.8 46.1 1.0 ND1 B:HIS134 4.9 39.1 1.0 C B:ASP227 4.9 42.3 1.0 C B:GLU228 4.9 46.2 1.0 O B:ASP227 4.9 42.1 1.0 O B:GLU228 5.0 46.1 1.0 FE B:FE402 5.0 1.0 1.0

Iron binding site 3 out of 3 in the Crystal Structure of Human P53R2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Human P53R2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe402 b:1.0 occ:1.00 OE2 B:GLU131 3.4 60.3 1.0 ND1 B:HIS134 3.4 39.1 1.0 CA B:ASP100 3.6 0.1 1.0 OE2 B:GLU228 3.6 55.3 1.0 CB B:HIS134 3.8 36.5 1.0 CG B:HIS134 4.1 38.1 1.0 CD B:GLU228 4.1 52.8 1.0 O B:HOH354 4.1 67.5 1.0 OE1 B:GLU228 4.2 55.3 1.0 C B:ASP100 4.5 1.0 1.0 CD B:GLU131 4.5 57.5 1.0 O B:ASP100 4.5 0.1 1.0 CE2 B:TYR138 4.5 40.2 1.0 CE1 B:HIS134 4.5 38.4 1.0 N B:ASP100 4.6 0.0 1.0 O B:GLU131 4.9 48.7 1.0 FE B:FE401 5.0 80.0 1.0

P.Smith, B.Zhou, N.Ho, Y.C.Yuan, L.Su, S.C.Tsai, Y.Yen. 2.6 A X-Ray Crystal Structure of Human P53R2, A P53-Inducible Ribonucleotide Reductase . Biochemistry V. 48 11134 2009.
ISSN: ISSN 0006-2960
PubMed: 19728742
DOI: 10.1021/BI9001425