Iron in PDB 5kjb: Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP

All present enzymatic activity of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP:
1.13.11.75;

The structure of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP, PDB code: 5kjb was solved by X.Sui, P.D.Kiser, K.Palczewski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.58 / 2.81
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	118.420, 124.950, 203.970, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 23.3

The binding sites of Iron atom in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP (pdb code 5kjb). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 5 binding sites of Iron where determined in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP, PDB code: 5kjb:
Jump to Iron binding site number: 1; 2; 3; 4; 5;

Iron binding site 1 out of 5 in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:85.6 occ:1.00 NE2 A:HIS304 2.7 61.3 1.0 NE2 A:HIS484 2.9 43.8 1.0 NE2 A:HIS183 2.9 51.8 1.0 CE1 A:HIS183 3.2 53.1 1.0 CE1 A:HIS304 3.3 59.6 1.0 CD2 A:HIS484 3.5 43.9 1.0 CD2 A:HIS304 3.9 58.8 1.0 CE1 A:HIS484 4.1 45.6 1.0 CD2 A:HIS183 4.3 48.0 1.0 CG2 A:THR136 4.4 31.2 1.0 CZ A:PHE303 4.5 90.8 1.0 ND1 A:HIS183 4.5 51.6 1.0 CE2 A:PHE303 4.6 87.3 1.0 ND1 A:HIS304 4.6 58.1 1.0 CG A:HIS484 4.8 39.7 1.0 CG A:HIS304 4.9 56.8 1.0

Iron binding site 2 out of 5 in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe501 b:86.4 occ:1.00 NE2 B:HIS304 2.9 55.9 1.0 NE2 B:HIS484 3.0 31.7 1.0 NE2 B:HIS183 3.1 45.3 1.0 CE1 B:HIS183 3.3 45.2 1.0 CE1 B:HIS304 3.4 57.4 1.0 CD2 B:HIS484 3.6 32.6 1.0 CD2 B:HIS304 4.1 52.4 1.0 CE1 B:HIS484 4.2 34.0 1.0 CZ B:PHE303 4.4 77.9 1.0 CG2 B:THR136 4.4 29.2 1.0 CD2 B:HIS183 4.4 40.5 1.0 CE2 B:PHE303 4.5 77.3 1.0 ND1 B:HIS183 4.6 41.0 1.0 ND1 B:HIS304 4.7 51.0 1.0 CG B:HIS484 4.9 34.4 1.0

Iron binding site 3 out of 5 in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe501 b:89.8 occ:1.00 NE2 C:HIS304 3.0 51.0 1.0 NE2 C:HIS484 3.1 42.6 1.0 NE2 C:HIS183 3.4 64.5 1.0 CE1 C:HIS304 3.5 51.7 1.0 CE1 C:HIS183 3.6 61.6 1.0 CD2 C:HIS484 3.6 41.5 1.0 CE1 C:HIS484 4.2 44.8 1.0 CD2 C:HIS304 4.3 50.8 1.0 CZ C:PHE303 4.4 75.7 1.0 CG2 C:THR136 4.4 39.3 1.0 CE2 C:PHE303 4.5 78.1 1.0 CD2 C:HIS183 4.7 57.8 1.0 ND1 C:HIS304 4.8 49.2 1.0 CD2 C:LEU483 4.8 42.4 1.0 ND1 C:HIS183 4.9 61.7 1.0 CG C:HIS484 4.9 41.5 1.0

Iron binding site 4 out of 5 in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe501 b:87.3 occ:1.00 NE2 D:HIS304 2.9 52.0 1.0 NE2 D:HIS484 2.9 47.5 1.0 NE2 D:HIS183 3.0 60.8 1.0 CE1 D:HIS183 3.2 58.2 1.0 CE1 D:HIS304 3.5 50.2 1.0 CD2 D:HIS484 3.5 46.2 1.0 CE1 D:HIS484 4.1 47.3 1.0 CD2 D:HIS304 4.1 52.0 1.0 CD2 D:HIS183 4.3 57.3 1.0 CG2 D:THR136 4.4 33.6 1.0 ND1 D:HIS183 4.5 53.7 1.0 CZ D:PHE303 4.6 71.5 1.0 CE2 D:PHE303 4.7 70.9 1.0 ND1 D:HIS304 4.7 47.7 1.0 CG D:HIS484 4.8 44.0 1.0

Iron binding site 5 out of 5 in the Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Synechocystis Apocarotenoid Oxygenase (Aco) Mutant - GLU150ASP within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe501 b:0.8 occ:1.00 NE2 E:HIS304 3.5 0.4 1.0 NE2 E:HIS484 3.6 0.9 1.0 NE2 E:HIS183 3.9 0.4 1.0 CE1 E:HIS304 3.9 0.2 1.0 CE1 E:HIS183 3.9 0.7 1.0 CD2 E:HIS484 4.0 0.4 1.0 CZ E:PHE303 4.2 0.2 1.0 CG2 E:THR136 4.3 88.8 1.0 CE2 E:PHE303 4.5 0.8 1.0 CD2 E:LEU483 4.6 0.1 1.0 CE1 E:HIS484 4.8 0.4 1.0 CD2 E:HIS304 4.8 0.6 1.0

X.Sui, J.Zhang, M.Golczak, K.Palczewski, P.D.Kiser. Key Residues For Catalytic Function and Metal Coordination in A Carotenoid Cleavage Dioxygenase. J.Biol.Chem. V. 291 19401 2016.
ISSN: ESSN 1083-351X
PubMed: 27453555
DOI: 10.1074/JBC.M116.744912