Iron in PDB 6son: 2 Minute FE2+ Soak Structure of Synftn Variant D137A

All present enzymatic activity of 2 Minute FE2+ Soak Structure of Synftn Variant D137A:
1.16.3.2;

The structure of 2 Minute FE2+ Soak Structure of Synftn Variant D137A, PDB code: 6son was solved by A.M.Hemmings, J.M.Bradley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	51.07 / 1.60
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	176.900, 176.900, 176.900, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 18.9

The structure of 2 Minute FE2+ Soak Structure of Synftn Variant D137A also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Iron atom in the 2 Minute FE2+ Soak Structure of Synftn Variant D137A (pdb code 6son). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the 2 Minute FE2+ Soak Structure of Synftn Variant D137A, PDB code: 6son:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the 2 Minute FE2+ Soak Structure of Synftn Variant D137A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of 2 Minute FE2+ Soak Structure of Synftn Variant D137A within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:48.2 occ:0.50 OE1 A:GLU33 2.0 34.1 0.6 O A:HOH364 2.1 75.3 1.0 OE1 A:GLU66 2.1 43.9 1.0 OE2 A:GLU66 2.4 47.0 1.0 CD A:GLU66 2.6 43.7 1.0 ND1 A:HIS69 2.7 39.6 1.0 CD A:GLU33 3.0 34.1 0.6 OE2 A:GLU33 3.3 34.8 0.6 OE2 A:GLU33 3.4 39.3 0.4 CD A:GLU33 3.5 38.2 0.4 FE A:FE202 3.5 70.7 0.4 CG A:HIS69 3.6 35.6 1.0 CG A:GLU33 3.6 35.6 0.4 CB A:HIS69 3.7 32.0 1.0 CE1 A:HIS69 3.7 40.9 1.0 CG2 A:THR113 3.8 35.7 1.0 O A:HOH304 4.0 50.9 1.0 OE1 A:GLN143 4.0 35.8 1.0 CG A:GLU66 4.0 39.3 1.0 O A:HOH334 4.2 40.9 1.0 OE1 A:GLU33 4.2 39.3 0.4 O A:HOH345 4.3 34.0 1.0 CG A:GLU33 4.3 32.2 0.6 CB A:GLU33 4.4 33.9 0.4 CA A:GLU66 4.5 29.6 1.0 CB A:GLU66 4.6 33.7 1.0 CB A:GLU33 4.6 31.1 0.6 OE2 A:GLU110 4.7 40.8 1.0 O A:HOH428 4.8 41.2 1.0 CD A:GLN143 4.8 33.3 1.0 O A:GLU66 4.8 28.4 1.0 CD2 A:HIS69 4.8 37.5 1.0 NE2 A:HIS69 4.8 39.2 1.0 CB A:THR113 4.9 35.0 1.0 OG1 A:THR113 5.0 36.4 1.0

Iron binding site 2 out of 2 in the 2 Minute FE2+ Soak Structure of Synftn Variant D137A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of 2 Minute FE2+ Soak Structure of Synftn Variant D137A within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe202 b:70.7 occ:0.36 OE2 A:GLU110 2.0 40.8 1.0 O A:HOH334 2.1 40.9 1.0 O A:HOH304 2.3 50.9 1.0 OE2 A:GLU66 2.3 47.0 1.0 OE1 A:GLU110 2.5 44.4 1.0 CD A:GLU110 2.5 40.9 1.0 CD A:GLU66 3.0 43.7 1.0 OE1 A:GLU66 3.5 43.9 1.0 FE A:FE201 3.5 48.2 0.5 OH A:TYR40 3.7 41.4 1.0 CG A:GLU66 3.9 39.3 1.0 CG A:GLU110 4.0 39.6 1.0 CE2 A:TYR40 4.1 34.1 1.0 OE1 A:GLN143 4.1 35.8 1.0 NE2 A:GLN143 4.2 33.4 1.0 OG1 A:THR113 4.3 36.4 1.0 CZ A:TYR40 4.4 38.1 1.0 CA A:GLU110 4.5 32.4 1.0 CG2 A:THR113 4.5 35.7 1.0 CB A:GLU110 4.5 35.0 1.0 CB A:THR113 4.5 35.0 1.0 CD A:GLN143 4.6 33.3 1.0 O A:HOH364 4.6 75.3 1.0 O A:HOH466 5.0 64.4 1.0

J.M.Bradley, J.Pullin, G.R.Moore, D.A.Svistunenko, A.M.Hemmings, N.E.Le Brun. Routes of Iron Entry Into, and Exit From, the Catalytic Ferroxidase Sites of the Prokaryotic Ferritin Synftn. Dalton Trans 2020.
ISSN: ESSN 1477-9234
PubMed: 31930254
DOI: 10.1039/C9DT03570B