Iron in PDB 6tp5: Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase

All present enzymatic activity of Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase:
1.14.11.29;

The structure of Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase, PDB code: 6tp5 was solved by M.Myllykoski, A.Sutinen, M.K.Koski, J.P.Kallio, A.Raasakka, J.Myllyharju, R.K.Wierenga, P.Koivunen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.30 / 2.25
Space group	P 31
Cell size a, b, c (Å), α, β, γ (°)	92.079, 92.079, 129.500, 90.00, 90.00, 120.00
R / Rfree (%)	18.2 / 22.1

The structure of Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Calcium	(Ca)	4 atoms

The binding sites of Iron atom in the Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase (pdb code 6tp5). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase, PDB code: 6tp5:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe702 b:80.2 occ:1.00 NE2 A:HIS441 2.2 56.8 1.0 OD1 A:ASP330 2.4 72.0 1.0 O1 A:OGA701 2.4 0.8 1.0 NE2 A:HIS328 2.4 60.1 1.0 O2' A:OGA701 2.4 0.1 1.0 C1 A:OGA701 2.8 0.4 1.0 C2 A:OGA701 2.9 0.4 1.0 CD2 A:HIS441 3.1 50.9 1.0 CG A:ASP330 3.2 71.5 1.0 CE1 A:HIS328 3.2 63.2 1.0 CE1 A:HIS441 3.2 54.6 1.0 OD2 A:ASP330 3.3 65.9 1.0 CD2 A:HIS328 3.4 50.5 1.0 O2 A:OGA701 3.9 0.1 1.0 N1 A:OGA701 4.2 82.6 1.0 CG A:HIS441 4.3 55.0 1.0 ND1 A:HIS441 4.3 52.1 1.0 ND1 A:HIS328 4.4 55.9 1.0 CG A:HIS328 4.5 52.1 1.0 CE2 A:TYR325 4.7 50.0 1.0 CB A:ASP330 4.7 62.7 1.0 CZ2 A:TRP457 4.8 53.7 1.0 CZ2 A:TRP421 4.9 57.9 1.0 C4 A:OGA701 4.9 74.3 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Human Transmembrane Prolyl 4-Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe702 b:85.6 occ:1.00 OD1 B:ASP330 2.2 85.6 1.0 O2 B:OGA701 2.2 0.9 1.0 NE2 B:HIS441 2.3 68.5 1.0 NE2 B:HIS328 2.4 59.2 1.0 O2' B:OGA701 2.4 0.1 1.0 C1 B:OGA701 2.8 0.8 1.0 C2 B:OGA701 2.9 99.8 1.0 CE1 B:HIS441 3.1 60.3 1.0 CG B:ASP330 3.1 73.8 1.0 OD2 B:ASP330 3.3 67.8 1.0 CE1 B:HIS328 3.3 55.5 1.0 CD2 B:HIS441 3.3 58.5 1.0 CD2 B:HIS328 3.4 56.8 1.0 O1 B:OGA701 4.0 0.1 1.0 ND1 B:HIS441 4.2 63.1 1.0 N1 B:OGA701 4.3 85.4 1.0 CG B:HIS441 4.3 60.4 1.0 ND1 B:HIS328 4.4 59.7 1.0 CG B:HIS328 4.5 57.0 1.0 CB B:ASP330 4.5 73.2 1.0 CE2 B:TYR325 4.7 54.9 1.0 CZ2 B:TRP457 4.8 73.8 1.0 CZ2 B:TRP421 4.9 65.0 1.0 CA B:ASP330 5.0 76.5 1.0

M.Myllykoski, A.Sutinen, M.K.Koski, J.Kallio, A.Raasakka, J.Myllyharju, R.Wierenga, P.Koivunen. Structure of Transmembrane Prolyl 4-Hydroxylase Reveals Unique Organization of Ef and Dioxygenase Domains J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X