Iron in PDB 6wfl: Camphor Soaked P450CAM D251E

All present enzymatic activity of Camphor Soaked P450CAM D251E:
1.14.15.1;

The structure of Camphor Soaked P450CAM D251E, PDB code: 6wfl was solved by J.A.Amaya, T.L.Poulos, D.Batabyal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.25 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	63.324, 64.039, 105.505, 90.00, 90.00, 90.00
R / Rfree (%)	21.8 / 25.4

The structure of Camphor Soaked P450CAM D251E also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Iron atom in the Camphor Soaked P450CAM D251E (pdb code 6wfl). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Camphor Soaked P450CAM D251E, PDB code: 6wfl:

Iron binding site 1 out of 1 in the Camphor Soaked P450CAM D251E


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Camphor Soaked P450CAM D251E within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe502 b:20.3 occ:1.00 FE A:HEM502 0.0 20.3 1.0 NA A:HEM502 2.0 18.1 1.0 NC A:HEM502 2.0 19.4 1.0 ND A:HEM502 2.0 17.3 1.0 NB A:HEM502 2.1 18.3 1.0 SG A:CYS357 2.4 21.9 1.0 C1C A:HEM502 3.0 18.7 1.0 C1A A:HEM502 3.0 18.7 1.0 C1D A:HEM502 3.1 22.7 1.0 C4D A:HEM502 3.1 18.6 1.0 C4A A:HEM502 3.1 19.4 1.0 C4B A:HEM502 3.1 20.8 1.0 O5 A:CAH503 3.1 37.5 0.9 C1B A:HEM502 3.1 19.9 1.0 C4C A:HEM502 3.1 21.8 1.0 CB A:CYS357 3.2 18.0 1.0 CHC A:HEM502 3.4 22.2 1.0 CHA A:HEM502 3.4 18.5 1.0 CHD A:HEM502 3.5 25.0 1.0 CHB A:HEM502 3.5 19.6 1.0 CA A:CYS357 4.0 20.4 1.0 C5 A:CAH503 4.1 34.9 0.9 C2C A:HEM502 4.2 20.0 1.0 C2D A:HEM502 4.3 25.6 1.0 C2A A:HEM502 4.3 21.2 1.0 C3A A:HEM502 4.3 17.3 1.0 C3D A:HEM502 4.3 22.4 1.0 C2B A:HEM502 4.3 18.5 1.0 C3B A:HEM502 4.3 19.9 1.0 C3C A:HEM502 4.3 21.8 1.0 N A:GLY359 4.5 21.5 1.0 C4 A:CAH503 4.5 40.2 0.9 C A:CYS357 4.7 21.7 1.0 N A:LEU358 4.7 21.8 1.0 CA A:GLY359 4.9 20.1 1.0

J.A.Amaya, D.Batabyal, T.L.Poulos. Proton Relay Network in the Bacterial P450S: CYP101A1 and CYP101D1. Biochemistry V. 59 2896 2020.
ISSN: ISSN 0006-2960
PubMed: 32574066
DOI: 10.1021/ACS.BIOCHEM.0C00329