Iron in PDB 7kq8: Structure of Iron Bound MEMO1

The structure of Structure of Iron Bound MEMO1, PDB code: 7kq8 was solved by M.T.Boniecki, E.E.Uhlemann, O.Y.Dmitriev, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.68 / 2.15
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	139.643, 88.861, 97.36, 90, 90, 90
R / Rfree (%)	15.9 / 20.2

The binding sites of Iron atom in the Structure of Iron Bound MEMO1 (pdb code 7kq8). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Structure of Iron Bound MEMO1, PDB code: 7kq8:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Structure of Iron Bound MEMO1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure of Iron Bound MEMO1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe317 b:25.1 occ:0.20 NE2 A:HIS81 2.5 26.5 1.0 O A:HOH538 2.5 37.2 1.0 O A:HOH485 2.6 30.1 1.0 SG A:CYS244 2.6 23.4 0.7 SG A:CYS244 2.7 21.9 0.3 NE2 A:HIS49 2.9 19.6 1.0 O32 A:GSH301 3.0 23.3 0.5 CB A:CYS244 3.1 21.3 0.3 CB A:CYS244 3.2 20.4 0.7 O32 A:GSH301 3.2 23.4 0.5 CE1 A:HIS81 3.4 22.6 1.0 CD2 A:HIS81 3.4 27.1 1.0 CE1 A:HIS49 3.7 18.1 1.0 C3 A:GSH301 3.9 26.6 0.5 CD2 A:HIS49 3.9 17.4 1.0 NE2 A:HIS192 4.0 18.5 1.0 C3 A:GSH301 4.1 26.6 0.5 CE1 A:HIS192 4.4 21.3 1.0 OD2 A:ASP189 4.4 23.8 1.0 ND1 A:HIS81 4.5 22.0 1.0 CA A:CYS244 4.5 21.3 0.7 CG A:HIS81 4.6 27.3 1.0 O31 A:GSH301 4.6 28.6 0.5 O A:HOH544 4.6 33.7 1.0 CA A:CYS244 4.6 21.5 0.3 NE2 A:HIS131 4.7 23.2 1.0 CA3 A:GSH301 4.7 26.3 0.5 O31 A:GSH301 4.8 28.7 0.5 ND1 A:HIS49 4.9 17.2 1.0 CA3 A:GSH301 4.9 26.1 0.5 CB A:ASP189 5.0 18.3 1.0 CG A:ASP189 5.0 20.3 1.0

Iron binding site 2 out of 4 in the Structure of Iron Bound MEMO1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure of Iron Bound MEMO1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe316 b:37.4 occ:0.41 SG B:CYS244 1.9 30.9 0.3 NE2 B:HIS49 2.3 21.9 1.0 NE2 B:HIS81 2.4 32.6 1.0 SG B:CYS244 2.7 27.5 0.7 O B:HOH470 2.9 30.9 1.0 CB B:CYS244 3.0 22.7 0.3 CB B:CYS244 3.0 23.2 0.7 CE1 B:HIS49 3.1 18.2 1.0 CE1 B:HIS81 3.1 29.9 1.0 O31 B:GSH301 3.4 25.9 0.5 CD2 B:HIS49 3.4 17.8 1.0 CD2 B:HIS81 3.5 32.5 1.0 O31 B:GSH301 3.5 26.1 0.4 OD2 B:ASP189 4.2 23.1 1.0 ND1 B:HIS49 4.3 20.3 1.0 C3 B:GSH301 4.3 29.1 0.5 NE2 B:HIS192 4.3 23.6 1.0 ND1 B:HIS81 4.3 24.0 1.0 CA B:CYS244 4.4 23.6 0.3 CG B:HIS49 4.5 18.6 1.0 C3 B:GSH301 4.5 29.1 0.4 CE1 B:HIS192 4.5 24.4 1.0 CA B:CYS244 4.6 23.7 0.7 CG B:HIS81 4.6 31.5 1.0 NE2 B:HIS131 4.6 23.5 1.0 CG B:ASP189 4.7 21.9 1.0 CB B:ASP189 4.7 20.0 1.0 CA3 B:GSH301 4.9 30.2 0.5

Iron binding site 3 out of 4 in the Structure of Iron Bound MEMO1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Structure of Iron Bound MEMO1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe311 b:38.8 occ:0.41 NE2 C:HIS49 2.3 23.0 1.0 NE2 C:HIS81 2.3 30.3 1.0 SG C:CYS244 2.6 29.9 1.0 O C:HOH483 2.9 30.7 1.0 CE1 C:HIS49 2.9 20.6 1.0 CB C:CYS244 2.9 30.2 1.0 CE1 C:HIS81 3.2 28.9 1.0 CD2 C:HIS81 3.4 26.7 1.0 O32 C:GSH301 3.5 25.9 0.4 CD2 C:HIS49 3.5 18.9 1.0 O32 C:GSH301 3.6 26.3 0.6 ND1 C:HIS49 4.1 21.6 1.0 ND1 C:HIS81 4.3 24.1 1.0 NE2 C:HIS192 4.4 18.6 1.0 CG C:HIS49 4.4 23.9 1.0 CA C:CYS244 4.4 27.6 1.0 OD2 C:ASP189 4.5 23.3 1.0 C3 C:GSH301 4.5 29.3 0.4 CG C:HIS81 4.5 31.3 1.0 CE1 C:HIS192 4.6 19.4 1.0 C3 C:GSH301 4.6 29.5 0.6 NE2 C:HIS131 4.6 24.2 1.0 CB C:ASP189 4.8 19.3 1.0 CG C:ASP189 4.8 24.4 1.0 CG C:PRO79 4.9 21.5 1.0 C C:CYS244 5.0 21.7 1.0

Iron binding site 4 out of 4 in the Structure of Iron Bound MEMO1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Structure of Iron Bound MEMO1 within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe312 b:33.5 occ:0.35 NE2 D:HIS81 2.4 27.1 1.0 SG D:CYS244 2.4 25.8 0.0 SG D:CYS244 2.4 29.3 1.0 NE2 D:HIS49 2.5 25.6 1.0 O D:HOH487 2.6 28.2 1.0 CB D:CYS244 3.0 25.2 0.0 CE1 D:HIS81 3.1 29.2 1.0 CE1 D:HIS49 3.2 16.9 1.0 CB D:CYS244 3.2 29.1 1.0 O31 D:GSH301 3.3 25.7 1.0 CD2 D:HIS81 3.5 29.1 1.0 CD2 D:HIS49 3.6 18.5 1.0 C3 D:GSH301 4.3 32.1 1.0 NE2 D:HIS192 4.3 18.7 1.0 ND1 D:HIS81 4.3 27.2 1.0 ND1 D:HIS49 4.4 22.2 1.0 CA D:CYS244 4.5 23.1 0.0 OD2 D:ASP189 4.5 23.4 1.0 CG D:HIS81 4.5 28.7 1.0 CE1 D:HIS192 4.5 18.6 1.0 CA D:CYS244 4.6 23.1 1.0 CG D:HIS49 4.7 21.8 1.0 NE2 D:HIS131 4.7 28.7 1.0 CB D:ASP189 4.9 15.1 1.0 CG D:ASP189 4.9 20.7 1.0 CA3 D:GSH301 5.0 31.4 1.0

N.V.Dolgova, E.E.Uhlemann, M.T.Boniecki, F.S.Vizeacoumar, F.J.Vizeacoumar, O.Y.Dmitriev. MEMO1 Is An Iron Containing Regulator of Metal Homeostasis in the Cell To Be Published.