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Iron in PDB 8gue: Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

Enzymatic activity of Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

All present enzymatic activity of Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238:
1.14.15.33;

Protein crystallography data

The structure of Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 8gue was solved by G.B.Li, Y.J.Pan, S.Y.Li, X.Gao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.77 / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 59.465, 97.818, 141.075, 90, 90, 90
R / Rfree (%) 20.1 / 25

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 (pdb code 8gue). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 8gue:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 8gue

Go back to Iron Binding Sites List in 8gue
Iron binding site 1 out of 2 in the Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe501

b:24.2
occ:1.00
FE A:HEM501 0.0 24.2 1.0
ND A:HEM501 2.0 24.0 1.0
NA A:HEM501 2.0 24.8 1.0
NC A:HEM501 2.0 23.6 1.0
NB A:HEM501 2.1 23.9 1.0
SG A:CYS354 2.3 22.6 1.0
C4C A:HEM501 3.0 21.8 1.0
C1D A:HEM501 3.0 24.3 1.0
C4D A:HEM501 3.1 22.2 1.0
C1A A:HEM501 3.1 24.4 1.0
C4B A:HEM501 3.1 25.6 1.0
C4A A:HEM501 3.1 22.6 1.0
C1C A:HEM501 3.1 24.1 1.0
C1B A:HEM501 3.1 28.8 1.0
CB A:CYS354 3.3 24.8 1.0
CHD A:HEM501 3.3 23.0 1.0
CHA A:HEM501 3.4 26.4 1.0
CHC A:HEM501 3.4 25.6 1.0
CHB A:HEM501 3.4 24.3 1.0
CA A:CYS354 4.0 24.5 1.0
C3C A:HEM501 4.3 23.7 1.0
C2D A:HEM501 4.3 24.4 1.0
C3B A:HEM501 4.3 28.6 1.0
C2A A:HEM501 4.3 26.8 1.0
C3D A:HEM501 4.3 24.3 1.0
C2C A:HEM501 4.3 21.6 1.0
C3A A:HEM501 4.3 21.2 1.0
C2B A:HEM501 4.3 25.8 1.0
O A:ALA243 4.5 42.2 1.0
C1 A:NRB502 4.5 29.3 1.0
CB A:ALA243 4.6 31.9 1.0
N A:GLY356 4.7 21.2 1.0
C A:CYS354 4.7 26.9 1.0
N A:ILE355 4.8 23.0 1.0
OG1 A:THR247 4.9 32.4 1.0

Iron binding site 2 out of 2 in 8gue

Go back to Iron Binding Sites List in 8gue
Iron binding site 2 out of 2 in the Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Narbomycin-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe501

b:24.2
occ:1.00
FE B:HEM501 0.0 24.2 1.0
ND B:HEM501 2.0 22.0 1.0
NA B:HEM501 2.0 24.8 1.0
NC B:HEM501 2.1 24.1 1.0
NB B:HEM501 2.1 22.6 1.0
SG B:CYS354 2.3 23.9 1.0
C4C B:HEM501 3.0 24.2 1.0
C1A B:HEM501 3.0 24.9 1.0
C1D B:HEM501 3.0 24.4 1.0
C4D B:HEM501 3.0 22.9 1.0
C4A B:HEM501 3.1 22.5 1.0
C4B B:HEM501 3.1 24.2 1.0
C1C B:HEM501 3.1 24.6 1.0
C1B B:HEM501 3.1 23.4 1.0
CB B:CYS354 3.2 25.0 1.0
CHD B:HEM501 3.4 21.6 1.0
CHA B:HEM501 3.4 24.8 1.0
CHC B:HEM501 3.4 25.1 1.0
CHB B:HEM501 3.4 24.5 1.0
CA B:CYS354 3.9 22.3 1.0
C3C B:HEM501 4.3 26.8 1.0
C2A B:HEM501 4.3 26.8 1.0
C3B B:HEM501 4.3 23.4 1.0
C3A B:HEM501 4.3 24.4 1.0
C2D B:HEM501 4.3 22.1 1.0
C3D B:HEM501 4.3 19.5 1.0
C2C B:HEM501 4.3 25.5 1.0
C2B B:HEM501 4.3 26.5 1.0
C1 B:NRB502 4.4 26.4 1.0
CB B:ALA243 4.6 26.0 1.0
O B:ALA243 4.6 40.1 1.0
N B:GLY356 4.7 22.5 1.0
C B:CYS354 4.7 26.2 1.0
N B:ILE355 4.8 27.6 1.0

Reference:

Y.J.Pan, G.B.Li, X.Gao, S.Y.Li. New Activities and Mechanistic Insight of Cytochrome P450 Pikc Gained From Site-Specific Mutagenesis By Non-Coding Amino Acids Nat Commun 2023.
ISSN: ESSN 2041-1723
Page generated: Sat Aug 10 05:00:51 2024

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