Iron in PDB 8jnc: Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin
Protein crystallography data
The structure of Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin, PDB code: 8jnc
was solved by
Y.L.Zhang,
L.P.Zhang,
C.S.Zhang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
13.29 /
2.00
|
Space group
|
I 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
91.895,
81.948,
143.838,
90,
94.3,
90
|
R / Rfree (%)
|
18.9 /
23.3
|
Other elements in 8jnc:
The structure of Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin
(pdb code 8jnc). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the
Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin, PDB code: 8jnc:
Jump to Iron binding site number:
1;
2;
Iron binding site 1 out
of 2 in 8jnc
Go back to
Iron Binding Sites List in 8jnc
Iron binding site 1 out
of 2 in the Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin
 Mono view
 Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe501
b:8.6
occ:1.00
|
FE
|
A:HEM501
|
0.0
|
8.6
|
1.0
|
NC
|
A:HEM501
|
2.0
|
7.1
|
1.0
|
ND
|
A:HEM501
|
2.1
|
7.8
|
1.0
|
NB
|
A:HEM501
|
2.1
|
9.6
|
1.0
|
NA
|
A:HEM501
|
2.1
|
4.8
|
1.0
|
SG
|
A:CYS357
|
2.3
|
6.9
|
1.0
|
C4C
|
A:HEM501
|
3.0
|
6.9
|
1.0
|
C1C
|
A:HEM501
|
3.0
|
4.9
|
1.0
|
C4B
|
A:HEM501
|
3.1
|
10.7
|
1.0
|
C1D
|
A:HEM501
|
3.1
|
4.2
|
1.0
|
C1A
|
A:HEM501
|
3.1
|
6.2
|
1.0
|
C4A
|
A:HEM501
|
3.1
|
5.6
|
1.0
|
C4D
|
A:HEM501
|
3.1
|
2.3
|
1.0
|
C1B
|
A:HEM501
|
3.1
|
8.4
|
1.0
|
HB2
|
A:CYS357
|
3.1
|
8.9
|
1.0
|
CB
|
A:CYS357
|
3.3
|
7.4
|
1.0
|
CHD
|
A:HEM501
|
3.4
|
7.7
|
1.0
|
CHC
|
A:HEM501
|
3.4
|
7.6
|
1.0
|
CHB
|
A:HEM501
|
3.4
|
8.4
|
1.0
|
CHA
|
A:HEM501
|
3.5
|
4.4
|
1.0
|
HC28
|
A:E5I502
|
3.6
|
14.5
|
1.0
|
HA
|
A:CYS357
|
3.7
|
7.4
|
1.0
|
HC31
|
A:E5I502
|
3.8
|
14.8
|
1.0
|
H
|
A:GLY359
|
3.9
|
7.0
|
1.0
|
CA
|
A:CYS357
|
4.0
|
6.2
|
1.0
|
HB3
|
A:CYS357
|
4.1
|
8.9
|
1.0
|
C3C
|
A:HEM501
|
4.2
|
6.3
|
1.0
|
C2C
|
A:HEM501
|
4.2
|
7.8
|
1.0
|
HB1
|
A:ALA245
|
4.2
|
11.9
|
1.0
|
C3B
|
A:HEM501
|
4.3
|
6.9
|
1.0
|
C3A
|
A:HEM501
|
4.3
|
4.2
|
1.0
|
C2A
|
A:HEM501
|
4.3
|
6.2
|
1.0
|
C2D
|
A:HEM501
|
4.3
|
9.2
|
1.0
|
C3D
|
A:HEM501
|
4.3
|
4.3
|
1.0
|
C2B
|
A:HEM501
|
4.3
|
6.8
|
1.0
|
HHD
|
A:HEM501
|
4.4
|
9.2
|
1.0
|
HHC
|
A:HEM501
|
4.4
|
9.1
|
1.0
|
HHB
|
A:HEM501
|
4.4
|
10.0
|
1.0
|
HHA
|
A:HEM501
|
4.4
|
5.2
|
1.0
|
HD1
|
A:PHE350
|
4.5
|
6.2
|
1.0
|
H30A
|
A:E5I502
|
4.5
|
18.4
|
1.0
|
C28
|
A:E5I502
|
4.6
|
12.2
|
1.0
|
H
|
A:ILE358
|
4.7
|
7.4
|
1.0
|
HA3
|
A:GLY359
|
4.7
|
10.0
|
1.0
|
C
|
A:CYS357
|
4.7
|
6.6
|
1.0
|
N
|
A:GLY359
|
4.7
|
5.9
|
1.0
|
C31
|
A:E5I502
|
4.7
|
12.3
|
1.0
|
HC29
|
A:E5I502
|
4.8
|
16.4
|
1.0
|
C30
|
A:E5I502
|
4.9
|
15.4
|
1.0
|
N
|
A:ILE358
|
4.9
|
6.2
|
1.0
|
|
Iron binding site 2 out
of 2 in 8jnc
Go back to
Iron Binding Sites List in 8jnc
Iron binding site 2 out
of 2 in the Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin
 Mono view
 Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Crystal Structure of Cytochrome P450 Ikad From Streptomyces Sp. ZJ306, in Complex with the Substrate 10-Epi-Maltophilin within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe501
b:10.2
occ:1.00
|
FE
|
B:HEM501
|
0.0
|
10.2
|
1.0
|
NB
|
B:HEM501
|
2.0
|
7.9
|
1.0
|
ND
|
B:HEM501
|
2.1
|
5.7
|
1.0
|
NA
|
B:HEM501
|
2.1
|
5.0
|
1.0
|
NC
|
B:HEM501
|
2.1
|
7.1
|
1.0
|
SG
|
B:CYS357
|
2.4
|
7.0
|
1.0
|
C4B
|
B:HEM501
|
3.0
|
8.8
|
1.0
|
C1D
|
B:HEM501
|
3.1
|
7.0
|
1.0
|
C1C
|
B:HEM501
|
3.1
|
5.6
|
1.0
|
C1B
|
B:HEM501
|
3.1
|
7.1
|
1.0
|
C4D
|
B:HEM501
|
3.1
|
6.1
|
1.0
|
C4C
|
B:HEM501
|
3.1
|
9.9
|
1.0
|
HB2
|
B:CYS357
|
3.1
|
8.4
|
1.0
|
C4A
|
B:HEM501
|
3.1
|
6.3
|
1.0
|
C1A
|
B:HEM501
|
3.1
|
8.5
|
1.0
|
CB
|
B:CYS357
|
3.3
|
7.0
|
1.0
|
CHC
|
B:HEM501
|
3.3
|
10.1
|
1.0
|
CHD
|
B:HEM501
|
3.4
|
9.6
|
1.0
|
CHB
|
B:HEM501
|
3.5
|
8.4
|
1.0
|
CHA
|
B:HEM501
|
3.5
|
6.5
|
1.0
|
HC28
|
B:E5I502
|
3.6
|
15.7
|
1.0
|
HA
|
B:CYS357
|
3.7
|
7.8
|
1.0
|
HC31
|
B:E5I502
|
3.8
|
11.9
|
1.0
|
CA
|
B:CYS357
|
4.1
|
6.5
|
1.0
|
H
|
B:GLY359
|
4.1
|
8.7
|
1.0
|
HB3
|
B:CYS357
|
4.1
|
8.4
|
1.0
|
C3B
|
B:HEM501
|
4.2
|
9.3
|
1.0
|
C2B
|
B:HEM501
|
4.3
|
9.5
|
1.0
|
HB1
|
B:ALA245
|
4.3
|
12.9
|
1.0
|
C2C
|
B:HEM501
|
4.3
|
5.7
|
1.0
|
C3C
|
B:HEM501
|
4.3
|
9.8
|
1.0
|
C2D
|
B:HEM501
|
4.3
|
8.0
|
1.0
|
H30A
|
B:E5I502
|
4.3
|
15.4
|
1.0
|
C3D
|
B:HEM501
|
4.3
|
6.2
|
1.0
|
HHC
|
B:HEM501
|
4.3
|
12.1
|
1.0
|
C3A
|
B:HEM501
|
4.3
|
8.6
|
1.0
|
C2A
|
B:HEM501
|
4.3
|
3.6
|
1.0
|
HD1
|
B:PHE350
|
4.4
|
6.0
|
1.0
|
HHD
|
B:HEM501
|
4.4
|
11.4
|
1.0
|
HHB
|
B:HEM501
|
4.4
|
10.1
|
1.0
|
HHA
|
B:HEM501
|
4.5
|
7.8
|
1.0
|
C28
|
B:E5I502
|
4.6
|
13.1
|
1.0
|
C31
|
B:E5I502
|
4.7
|
10.0
|
1.0
|
C30
|
B:E5I502
|
4.8
|
12.9
|
1.0
|
HA3
|
B:GLY359
|
4.8
|
11.9
|
1.0
|
H
|
B:ILE358
|
4.9
|
5.7
|
1.0
|
C
|
B:CYS357
|
4.9
|
5.5
|
1.0
|
N
|
B:GLY359
|
4.9
|
7.3
|
1.0
|
HC29
|
B:E5I502
|
5.0
|
13.0
|
1.0
|
|
Reference:
P.Jiang,
H.Jin,
G.Zhang,
W.Zhang,
W.Liu,
Y.Zhu,
C.Zhang,
L.Zhang.
A Mechanistic Understanding of the Distinct Regio- and Chemoselectivity of Multifunctional P450S By Structural Comparison of Ikad and Cfta Complexed with Common Substrates. Angew.Chem.Int.Ed.Engl. 10728 2023.
ISSN: ESSN 1521-3773
PubMed: 37917570
DOI: 10.1002/ANIE.202310728
Page generated: Sat Aug 10 07:19:31 2024
|