Iron in PDB 9ne7: Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State

All present enzymatic activity of Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State:
2.7.7.7;

The binding sites of Iron atom in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State (pdb code 9ne7). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State, PDB code: 9ne7:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1301 b:101.2 occ:1.00 FE1 A:SF41301 0.0 101.2 1.0 SG A:CYS651 2.3 90.0 1.0 S2 A:SF41301 2.3 101.2 1.0 S4 A:SF41301 2.3 101.2 1.0 S3 A:SF41301 2.3 101.2 1.0 FE4 A:SF41301 2.7 101.2 1.0 FE3 A:SF41301 2.7 101.2 1.0 FE2 A:SF41301 2.7 101.2 1.0 CB A:CYS651 2.8 90.0 1.0 CA A:CYS651 3.4 90.0 1.0 S1 A:SF41301 3.9 101.2 1.0 O A:CYS651 4.3 90.0 1.0 C A:CYS651 4.4 90.0 1.0 CB A:CYS654 4.4 90.0 1.0 N A:CYS651 4.5 90.0 1.0 SG A:CYS654 4.6 90.0 1.0 SG A:CYS747 4.8 65.8 1.0 SG A:CYS663 4.8 84.8 1.0

Iron binding site 2 out of 4 in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1301 b:101.2 occ:1.00 FE2 A:SF41301 0.0 101.2 1.0 SG A:CYS654 2.3 90.0 1.0 S3 A:SF41301 2.3 101.2 1.0 S1 A:SF41301 2.3 101.2 1.0 S4 A:SF41301 2.3 101.2 1.0 FE4 A:SF41301 2.7 101.2 1.0 FE3 A:SF41301 2.7 101.2 1.0 FE1 A:SF41301 2.7 101.2 1.0 CB A:CYS654 3.1 90.0 1.0 CB A:PHE656 3.7 90.1 1.0 S2 A:SF41301 3.9 101.2 1.0 C A:PHE656 4.3 90.1 1.0 CA A:PHE656 4.4 90.1 1.0 N A:PHE656 4.4 90.1 1.0 CA A:CYS654 4.5 90.0 1.0 N A:ASN657 4.6 90.4 1.0 O A:PHE656 4.6 90.1 1.0 O A:CYS651 4.6 90.0 1.0 SG A:CYS663 4.7 84.8 1.0 CB A:CYS663 4.7 84.8 1.0 SG A:CYS747 4.8 65.8 1.0 CA A:CYS651 4.8 90.0 1.0 C A:CYS654 4.8 90.0 1.0 CB A:CYS651 4.9 90.0 1.0 CD1 A:LEU145 4.9 82.7 1.0 CG A:PHE656 4.9 90.1 1.0 SG A:CYS651 4.9 90.0 1.0

Iron binding site 3 out of 4 in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1301 b:101.2 occ:1.00 FE3 A:SF41301 0.0 101.2 1.0 SG A:CYS663 2.3 84.8 1.0 S1 A:SF41301 2.3 101.2 1.0 S2 A:SF41301 2.3 101.2 1.0 S4 A:SF41301 2.3 101.2 1.0 CB A:CYS663 2.5 84.8 1.0 FE4 A:SF41301 2.7 101.2 1.0 FE2 A:SF41301 2.7 101.2 1.0 FE1 A:SF41301 2.7 101.2 1.0 CA A:CYS663 3.8 84.8 1.0 S3 A:SF41301 3.9 101.2 1.0 O A:CYS663 4.0 84.8 1.0 C A:CYS663 4.0 84.8 1.0 O A:PHE656 4.2 90.1 1.0 CG A:GLN664 4.5 81.4 1.0 C A:PHE656 4.6 90.1 1.0 SG A:CYS747 4.6 65.8 1.0 CB A:PHE656 4.7 90.1 1.0 N A:CYS663 4.8 84.8 1.0 N A:GLN664 4.8 81.4 1.0 SG A:CYS651 4.8 90.0 1.0 SG A:CYS654 4.9 90.0 1.0 N A:ASN657 5.0 90.4 1.0 CA A:ASN657 5.0 90.4 1.0 CB A:CYS651 5.0 90.0 1.0

Iron binding site 4 out of 4 in the Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Human Polymerase Epsilon Bound to Pcna and Dna with An in-Situ- Generated Mismatch in the Pol-Backtracking State within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1301 b:101.2 occ:1.00 FE4 A:SF41301 0.0 101.2 1.0 SG A:CYS747 2.2 65.8 1.0 S1 A:SF41301 2.3 101.2 1.0 S2 A:SF41301 2.3 101.2 1.0 S3 A:SF41301 2.3 101.2 1.0 FE3 A:SF41301 2.7 101.2 1.0 FE2 A:SF41301 2.7 101.2 1.0 FE1 A:SF41301 2.7 101.2 1.0 CB A:CYS747 3.2 65.8 1.0 S4 A:SF41301 3.9 101.2 1.0 CB A:CYS663 4.0 84.8 1.0 CA A:CYS747 4.1 65.8 1.0 O A:CYS663 4.2 84.8 1.0 SG A:CYS651 4.6 90.0 1.0 O A:ARG749 4.7 77.9 1.0 SG A:CYS654 4.7 90.0 1.0 SG A:CYS663 4.7 84.8 1.0 C A:CYS663 4.9 84.8 1.0 CA A:CYS663 5.0 84.8 1.0 CD1 A:LEU145 5.0 82.7 1.0 C A:CYS747 5.0 65.8 1.0

F.Wang, Q.He, M.E.O'donnell, H.Li. The Proofreading Mechanism of the Human Leading-Strand Dna Polymerase Epsilon Holoenzyme. Proc.Natl.Acad.Sci.Usa V. 122 32122 2025.
ISSN: ESSN 1091-6490
PubMed: 40440070
DOI: 10.1073/PNAS.2507232122