Iron in PDB 1b0v: I40N Mutant of Azotobacter Vinelandii Fdi

The structure of I40N Mutant of Azotobacter Vinelandii Fdi, PDB code: 1b0v was solved by V.Sridhar, G.S.Prasad, C.D.Stout, K.Chen, B.K.Burgess, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.80
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	42.000, 55.300, 62.300, 78.10, 85.20, 71.90
R / Rfree (%)	20.9 / n/a

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 28;

Binding sites:

The binding sites of Iron atom in the I40N Mutant of Azotobacter Vinelandii Fdi (pdb code 1b0v). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 28 binding sites of Iron where determined in the I40N Mutant of Azotobacter Vinelandii Fdi, PDB code: 1b0v:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Iron binding site 1 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe107 b:22.4 occ:1.00 FE1 A:SF4107 0.0 22.4 1.0 S2 A:SF4107 2.2 22.4 1.0 S3 A:SF4107 2.2 22.4 1.0 S4 A:SF4107 2.3 24.7 1.0 SG A:CYS39 2.4 24.3 1.0 FE3 A:SF4107 2.5 22.9 1.0 FE4 A:SF4107 2.5 23.7 1.0 FE2 A:SF4107 2.6 25.4 1.0 CB A:CYS39 3.3 24.9 1.0 CA A:CYS39 3.7 27.7 1.0 S1 A:SF4107 3.8 22.2 1.0 SG A:CYS24 3.9 26.0 1.0 C A:CYS39 4.3 27.6 1.0 N A:ASN40 4.3 28.2 1.0 CB A:PHE2 4.5 27.7 1.0 CG2 A:ILE34 4.6 23.8 1.0 N A:ASP41 4.6 33.0 1.0 SG A:CYS45 4.6 22.3 1.0 SG A:CYS20 4.7 22.2 1.0 N A:CYS42 4.8 29.2 1.0 CA A:ASP41 4.9 33.5 1.0 SG A:CYS42 4.9 24.3 1.0 N A:CYS39 4.9 27.0 1.0 N A:PHE2 4.9 25.5 1.0 CD1 A:PHE2 5.0 30.5 1.0

Iron binding site 2 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe107 b:25.4 occ:1.00 FE2 A:SF4107 0.0 25.4 1.0 S3 A:SF4107 2.3 22.4 1.0 S1 A:SF4107 2.3 22.2 1.0 S4 A:SF4107 2.3 24.7 1.0 SG A:CYS42 2.4 24.3 1.0 FE3 A:SF4107 2.6 22.9 1.0 FE4 A:SF4107 2.6 23.7 1.0 FE1 A:SF4107 2.6 22.4 1.0 CB A:CYS42 3.6 26.8 1.0 N A:CYS42 3.6 29.2 1.0 S2 A:SF4107 3.8 22.4 1.0 N A:ALA43 4.0 25.0 1.0 CA A:CYS42 4.0 27.2 1.0 CD A:PRO21 4.1 18.1 1.0 C A:CYS42 4.2 26.3 1.0 N A:LEU44 4.5 24.1 1.0 SG A:CYS45 4.5 22.3 1.0 OD1 A:ASN40 4.5 38.0 1.0 SG A:CYS20 4.6 22.2 1.0 SG A:CYS39 4.6 24.3 1.0 C A:ASP41 4.7 33.1 1.0 CG A:PRO21 4.7 18.0 1.0 CA A:ALA43 4.8 24.7 1.0 N A:CYS45 4.9 24.7 1.0 N A:ASP41 5.0 33.0 1.0 N A:ASN40 5.0 28.2 1.0

Iron binding site 3 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe107 b:22.9 occ:1.00 FE3 A:SF4107 0.0 22.9 1.0 S4 A:SF4107 2.3 24.7 1.0 S2 A:SF4107 2.3 22.4 1.0 S1 A:SF4107 2.3 22.2 1.0 SG A:CYS45 2.3 22.3 1.0 FE1 A:SF4107 2.5 22.4 1.0 FE2 A:SF4107 2.6 25.4 1.0 FE4 A:SF4107 2.6 23.7 1.0 CB A:CYS45 3.3 24.8 1.0 S3 A:SF4107 3.8 22.4 1.0 N A:CYS45 4.1 24.7 1.0 CE2 A:PHE25 4.3 15.7 1.0 CA A:CYS45 4.4 24.5 1.0 CZ A:PHE25 4.4 13.5 1.0 SG A:CYS42 4.5 24.3 1.0 SG A:CYS20 4.6 22.2 1.0 CB A:PHE2 4.6 27.7 1.0 SG A:CYS39 4.6 24.3 1.0 CD1 A:ILE34 4.7 21.6 1.0 N A:LEU44 4.9 24.1 1.0 N A:ALA43 5.0 25.0 1.0 CG A:PHE2 5.0 30.2 1.0

Iron binding site 4 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe107 b:23.7 occ:1.00 FE4 A:SF4107 0.0 23.7 1.0 S3 A:SF4107 2.2 22.4 1.0 S2 A:SF4107 2.3 22.4 1.0 S1 A:SF4107 2.3 22.2 1.0 SG A:CYS20 2.3 22.2 1.0 FE1 A:SF4107 2.5 22.4 1.0 FE2 A:SF4107 2.6 25.4 1.0 FE3 A:SF4107 2.6 22.9 1.0 CB A:CYS20 3.4 21.9 1.0 SG A:CYS24 3.4 26.0 1.0 S4 A:SF4107 3.8 24.7 1.0 CA A:CYS20 3.9 23.2 1.0 CD A:PRO21 4.2 18.1 1.0 CE2 A:PHE25 4.2 15.7 1.0 CG2 A:VAL22 4.3 13.4 1.0 SG A:CYS42 4.6 24.3 1.0 N A:PRO21 4.7 21.6 1.0 C A:CYS20 4.7 22.5 1.0 SG A:CYS45 4.8 22.3 1.0 SG A:CYS39 4.8 24.3 1.0 CD2 A:PHE25 4.9 16.9 1.0 N A:CYS20 5.0 25.2 1.0 CZ A:PHE25 5.0 13.5 1.0

Iron binding site 5 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe108 b:21.5 occ:1.00 FE1 A:F3S108 0.0 21.5 1.0 S2 A:F3S108 2.3 19.2 1.0 S3 A:F3S108 2.3 18.6 1.0 SG A:CYS16 2.3 21.0 1.0 S1 A:F3S108 2.3 21.4 1.0 FE4 A:F3S108 2.5 18.7 1.0 FE3 A:F3S108 2.6 19.3 1.0 CB A:CYS16 3.3 23.0 1.0 S4 A:F3S108 3.8 18.5 1.0 N A:THR14 3.8 25.6 1.0 CA A:THR14 3.9 25.1 1.0 N A:CYS16 4.1 24.2 1.0 CD2 A:LEU32 4.2 8.3 1.0 CA A:CYS16 4.3 24.8 1.0 C A:THR14 4.4 25.2 1.0 N A:ASP15 4.5 26.0 1.0 SG A:CYS49 4.7 16.6 1.0 CD1 A:ILE54 4.7 16.0 1.0 SG A:CYS8 4.7 15.6 1.0 N A:TYR13 4.9 23.0 1.0 CB A:LEU32 4.9 13.0 1.0 C A:TYR13 5.0 25.0 1.0

Iron binding site 6 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe108 b:19.3 occ:1.00 FE3 A:F3S108 0.0 19.3 1.0 S4 A:F3S108 2.2 18.5 1.0 SG A:CYS49 2.3 16.6 1.0 S1 A:F3S108 2.3 21.4 1.0 S3 A:F3S108 2.3 18.6 1.0 FE1 A:F3S108 2.6 21.5 1.0 FE4 A:F3S108 2.7 18.7 1.0 CB A:CYS49 3.4 19.8 1.0 CA A:CYS49 3.9 22.1 1.0 S2 A:F3S108 4.0 19.2 1.0 CD A:PRO50 4.4 24.4 1.0 C A:CYS49 4.6 23.6 1.0 CD2 A:TYR13 4.6 29.3 1.0 SG A:CYS16 4.6 21.0 1.0 CB A:TYR13 4.6 24.0 1.0 N A:PRO50 4.7 24.4 1.0 CB A:ALA51 4.7 24.7 1.0 SG A:CYS8 4.7 15.6 1.0 CB A:CYS16 4.8 23.0 1.0 N A:ALA51 4.8 26.0 1.0 CD1 A:ILE54 4.9 16.0 1.0 CB A:CYS8 4.9 17.2 1.0 N A:TYR13 4.9 23.0 1.0

Iron binding site 7 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe108 b:18.7 occ:1.00 FE4 A:F3S108 0.0 18.7 1.0 S3 A:F3S108 2.2 18.6 1.0 S2 A:F3S108 2.3 19.2 1.0 S4 A:F3S108 2.3 18.5 1.0 SG A:CYS8 2.3 15.6 1.0 FE1 A:F3S108 2.5 21.5 1.0 FE3 A:F3S108 2.7 19.3 1.0 CB A:CYS8 3.2 17.2 1.0 S1 A:F3S108 3.9 21.4 1.0 CA A:LYS12 4.0 22.6 1.0 CA A:CYS8 4.0 16.2 1.0 N A:TYR13 4.3 23.0 1.0 N A:LYS12 4.3 22.4 1.0 CG1 A:VAL4 4.4 6.0 1.0 O A:CYS8 4.6 14.4 1.0 CB A:LEU32 4.6 13.0 1.0 C A:LYS12 4.7 21.9 1.0 SG A:CYS16 4.7 21.0 1.0 SG A:CYS49 4.7 16.6 1.0 N A:THR14 4.7 25.6 1.0 C A:CYS8 4.7 16.1 1.0 CB A:LYS12 5.0 23.0 1.0

Iron binding site 8 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe307 b:21.0 occ:1.00 FE1 B:SF4307 0.0 21.0 1.0 S2 B:SF4307 2.3 23.7 1.0 S4 B:SF4307 2.3 23.7 1.0 S3 B:SF4307 2.3 22.3 1.0 SG B:CYS239 2.3 25.5 1.0 FE3 B:SF4307 2.5 26.1 1.0 FE4 B:SF4307 2.7 22.0 1.0 FE2 B:SF4307 2.8 24.7 1.0 CB B:CYS239 3.5 27.2 1.0 S1 B:SF4307 3.9 24.3 1.0 SG B:CYS224 4.0 26.4 1.0 CA B:CYS239 4.0 27.4 1.0 N B:ASP241 4.3 28.1 1.0 N B:ASN240 4.4 26.5 1.0 CB B:PHE202 4.4 25.9 1.0 SG B:CYS245 4.4 24.5 1.0 CG2 B:ILE234 4.5 15.9 1.0 C B:CYS239 4.6 26.6 1.0 CD1 B:PHE202 4.7 24.0 1.0 CA B:ASP241 4.8 26.9 1.0 CB B:ASN240 4.8 27.7 1.0 CG B:PHE202 4.8 24.7 1.0 N B:PHE202 4.8 24.2 1.0 N B:CYS242 4.8 23.8 1.0 SG B:CYS220 4.9 27.0 1.0

Iron binding site 9 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 9 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe307 b:24.7 occ:1.00 FE2 B:SF4307 0.0 24.7 1.0 S4 B:SF4307 2.3 23.7 1.0 S1 B:SF4307 2.3 24.3 1.0 SG B:CYS242 2.3 22.2 1.0 S3 B:SF4307 2.3 22.3 1.0 FE3 B:SF4307 2.6 26.1 1.0 FE4 B:SF4307 2.8 22.0 1.0 FE1 B:SF4307 2.8 21.0 1.0 CB B:CYS242 3.5 23.4 1.0 N B:CYS242 3.6 23.8 1.0 N B:ALA243 3.8 15.2 1.0 CA B:CYS242 3.9 20.9 1.0 S2 B:SF4307 3.9 23.7 1.0 C B:CYS242 4.1 18.1 1.0 CB B:ASN240 4.1 27.7 1.0 CD B:PRO221 4.3 26.8 1.0 N B:LEU244 4.3 18.6 1.0 SG B:CYS245 4.5 24.5 1.0 N B:ASP241 4.6 28.1 1.0 SG B:CYS239 4.6 25.5 1.0 CA B:ALA243 4.7 15.3 1.0 C B:ASP241 4.8 26.4 1.0 SG B:CYS220 4.8 27.0 1.0 OD1 B:ASN240 4.8 34.1 1.0 CG B:PRO221 4.8 25.6 1.0 CB B:LEU244 4.9 24.7 1.0 N B:CYS245 4.9 24.8 1.0 C B:ALA243 5.0 16.2 1.0 CA B:ASP241 5.0 26.9 1.0 CG B:ASN240 5.0 29.7 1.0

Iron binding site 10 out of 28 in the I40N Mutant of Azotobacter Vinelandii Fdi


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 10 of I40N Mutant of Azotobacter Vinelandii Fdi within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe307 b:26.1 occ:1.00 FE3 B:SF4307 0.0 26.1 1.0 S1 B:SF4307 2.3 24.3 1.0 SG B:CYS245 2.3 24.5 1.0 S4 B:SF4307 2.3 23.7 1.0 S2 B:SF4307 2.3 23.7 1.0 FE1 B:SF4307 2.5 21.0 1.0 FE2 B:SF4307 2.6 24.7 1.0 FE4 B:SF4307 2.6 22.0 1.0 CB B:CYS245 3.3 26.7 1.0 S3 B:SF4307 3.6 22.3 1.0 N B:CYS245 4.1 24.8 1.0 CE2 B:PHE225 4.3 13.2 1.0 CZ B:PHE225 4.3 12.5 1.0 CA B:CYS245 4.3 25.2 1.0 SG B:CYS242 4.6 22.2 1.0 SG B:CYS220 4.6 27.0 1.0 SG B:CYS239 4.7 25.5 1.0 CB B:PHE202 4.8 25.9 1.0 N B:LEU244 4.8 18.6 1.0 CD1 B:ILE234 4.8 17.6 1.0 N B:ALA243 4.9 15.2 1.0 CB B:CYS220 4.9 27.1 1.0

K.Chen, G.J.Tilley, V.Sridhar, G.S.Prasad, C.D.Stout, F.A.Armstrong, B.K.Burgess. Alteration of the Reduction Potential of the [4FE-4S](2+/+) Cluster of Azotobacter Vinelandii Ferredoxin I. J.Biol.Chem. V. 274 36479 1999.
ISSN: ISSN 0021-9258
PubMed: 10593945
DOI: 10.1074/JBC.274.51.36479