Iron in PDB 1b2o: Clostridium Pasteurianum Rubredoxin G10VG43A Mutant

The structure of Clostridium Pasteurianum Rubredoxin G10VG43A Mutant, PDB code: 1b2o was solved by M.J.Maher, J.M.Guss, M.C.J.Wilce, A.G.Wedd, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.90
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	61.850, 61.850, 80.450, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 23.7

The binding sites of Iron atom in the Clostridium Pasteurianum Rubredoxin G10VG43A Mutant (pdb code 1b2o). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Clostridium Pasteurianum Rubredoxin G10VG43A Mutant, PDB code: 1b2o:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Clostridium Pasteurianum Rubredoxin G10VG43A Mutant


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Clostridium Pasteurianum Rubredoxin G10VG43A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe55 b:18.7 occ:1.00 SG A:CYS9 2.3 15.8 1.0 SG A:CYS42 2.3 19.2 1.0 SG A:CYS6 2.3 17.3 1.0 SG A:CYS39 2.3 17.8 1.0 CB A:CYS6 3.2 14.2 1.0 CB A:CYS39 3.3 18.2 1.0 CB A:CYS9 3.3 12.5 1.0 CB A:CYS42 3.4 15.5 1.0 N A:CYS42 3.8 18.2 1.0 N A:CYS9 3.9 17.3 1.0 CA A:CYS42 4.1 19.1 1.0 CA A:CYS9 4.1 14.3 1.0 CG2 A:VAL44 4.3 18.5 1.0 CB A:TYR11 4.4 13.5 1.0 C A:CYS9 4.6 17.1 1.0 CA A:CYS6 4.7 15.2 1.0 C A:VAL8 4.7 18.1 1.0 CA A:CYS39 4.7 14.8 1.0 C A:CYS42 4.7 21.0 1.0 O A:CYS9 4.8 14.3 1.0 CG1 A:VAL8 4.8 30.2 1.0 CB A:LEU41 4.9 19.8 1.0 C A:LEU41 4.9 20.9 1.0 N A:ALA43 4.9 17.8 1.0 CZ A:PHE49 4.9 18.1 1.0

Iron binding site 2 out of 2 in the Clostridium Pasteurianum Rubredoxin G10VG43A Mutant


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Clostridium Pasteurianum Rubredoxin G10VG43A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe55 b:18.0 occ:1.00 SG B:CYS9 2.2 16.0 1.0 SG B:CYS42 2.2 15.0 1.0 SG B:CYS6 2.3 16.5 1.0 SG B:CYS39 2.3 14.8 1.0 CB B:CYS39 3.2 12.0 1.0 CB B:CYS6 3.3 13.8 1.0 CB B:CYS42 3.3 16.6 1.0 CB B:CYS9 3.4 17.2 1.0 N B:CYS42 3.7 15.2 1.0 N B:CYS9 3.8 15.7 1.0 CA B:CYS42 4.1 14.1 1.0 CA B:CYS9 4.1 13.6 1.0 CG2 B:VAL44 4.3 9.9 1.0 CB B:TYR11 4.4 15.4 1.0 C B:CYS9 4.6 15.7 1.0 CB B:VAL8 4.6 19.7 1.0 C B:VAL8 4.7 22.3 1.0 CA B:CYS39 4.7 15.1 1.0 CA B:CYS6 4.7 17.0 1.0 O B:CYS9 4.7 17.5 1.0 CB B:LEU41 4.8 16.6 1.0 C B:CYS42 4.8 15.5 1.0 C B:LEU41 4.8 18.8 1.0 N B:ALA43 4.8 13.2 1.0 N B:TYR11 5.0 14.5 1.0

M.J.Maher, Z.Xiao, M.C.Wilce, J.M.Guss, A.G.Wedd. Rubredoxin From Clostridium Pasteurianum. Structures of G10A, G43A and G10VG43A Mutant Proteins. Mutation of Conserved Glycine 10 to Valine Causes the 9-10 Peptide Link to Invert. Acta Crystallogr.,Sect.D V. 55 962 1999.
ISSN: ISSN 0907-4449
PubMed: 10216292
DOI: 10.1107/S0907444999001900