Iron in PDB 1b3e: Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris

The structure of Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris, PDB code: 1b3e was solved by M.C.Bewley, B.M.Tam, J.Grewal, S.He, S.Shewry, M.E.P.Murphy, A.B.Mason, R.C.Woodworth, E.N.Baker, R.T.A.Macgillivray, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.50
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	73.500, 73.500, 156.700, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / 25.5

The binding sites of Iron atom in the Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris (pdb code 1b3e). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris, PDB code: 1b3e:

Iron binding site 1 out of 1 in the Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Human Serum Transferrin, N-Terminal Lobe, Expressed in Pichia Pastoris within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe400 b:20.8 occ:1.00 OH A:TYR95 1.9 19.4 1.0 OH A:TYR188 1.9 22.9 1.0 OD1 A:ASP63 2.0 25.9 1.0 O1 A:CO3401 2.0 18.9 1.0 O2 A:CO3401 2.0 21.0 1.0 NE2 A:HIS249 2.1 23.4 1.0 C A:CO3401 2.4 24.4 1.0 CZ A:TYR95 3.0 26.0 1.0 CZ A:TYR188 3.0 19.1 1.0 CE1 A:HIS249 3.0 19.3 1.0 CG A:ASP63 3.1 21.8 1.0 CD2 A:HIS249 3.1 23.1 1.0 CB A:ASP63 3.7 21.3 1.0 CE2 A:TYR95 3.7 27.3 1.0 CE1 A:TYR188 3.7 14.9 1.0 O3 A:CO3401 3.7 21.1 1.0 O A:HOH420 3.8 14.0 1.0 CE2 A:TYR188 3.9 16.9 1.0 CE1 A:TYR95 3.9 23.6 1.0 OD2 A:ASP63 4.1 27.1 1.0 ND1 A:HIS249 4.2 18.6 1.0 CG A:HIS249 4.2 23.9 1.0 NZ A:LYS296 4.3 14.2 1.0 NH2 A:ARG124 4.4 13.9 1.0 CA A:ASP63 4.4 24.4 1.0 CB A:SER125 4.6 21.5 1.0 NE A:ARG124 4.8 15.1 1.0 CD A:LYS296 4.8 13.8 1.0 N A:ALA126 4.9 19.4 1.0 N A:SER125 4.9 15.9 1.0

M.C.Bewley, B.M.Tam, J.Grewal, S.He, S.Shewry, M.E.Murphy, A.B.Mason, R.C.Woodworth, E.N.Baker, R.T.Macgillivray. X-Ray Crystallography and Mass Spectroscopy Reveal That the N-Lobe of Human Transferrin Expressed in Pichia Pastoris Is Folded Correctly But Is Glycosylated on Serine-32. Biochemistry V. 38 2535 1999.
ISSN: ISSN 0006-2960
PubMed: 10029548
DOI: 10.1021/BI9824543