Iron in PDB 1c8j: Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F)

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F):
1.14.15.1;

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F), PDB code: 1c8j was solved by Y.Liu, F.Jiang, Q.Guo, X.Chen, J.Jin, Y.Sun, Z.Rao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	100.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.860, 61.990, 95.130, 90.00, 90.54, 90.00
R / Rfree (%)	19.3 / 26.4

The binding sites of Iron atom in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F) (pdb code 1c8j). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F), PDB code: 1c8j:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F) within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe417 b:29.9 occ:1.00 FE A:HEM417 0.0 29.9 1.0 NA A:HEM417 2.0 27.3 1.0 NB A:HEM417 2.1 28.0 1.0 NC A:HEM417 2.1 29.5 1.0 ND A:HEM417 2.1 29.4 1.0 SG A:CYS357 2.3 31.6 1.0 C1D A:HEM417 3.0 26.7 1.0 C4B A:HEM417 3.1 27.0 1.0 C4C A:HEM417 3.1 28.7 1.0 C4A A:HEM417 3.1 26.9 1.0 C4D A:HEM417 3.1 27.0 1.0 C1C A:HEM417 3.1 28.7 1.0 C1B A:HEM417 3.1 28.0 1.0 C1A A:HEM417 3.1 27.2 1.0 CB A:CYS357 3.2 24.8 1.0 CHC A:HEM417 3.4 27.5 1.0 CHD A:HEM417 3.5 29.6 1.0 CHA A:HEM417 3.5 25.6 1.0 CHB A:HEM417 3.5 27.2 1.0 CA A:CYS357 4.0 27.2 1.0 C2D A:HEM417 4.3 27.8 1.0 C3D A:HEM417 4.3 26.0 1.0 C2B A:HEM417 4.3 27.1 1.0 C3B A:HEM417 4.3 26.3 1.0 C3C A:HEM417 4.3 30.7 1.0 C2C A:HEM417 4.3 29.8 1.0 C3A A:HEM417 4.3 24.6 1.0 C2A A:HEM417 4.3 24.3 1.0 N A:GLY359 4.5 25.4 1.0 C A:CYS357 4.7 25.9 1.0 N A:LEU358 4.7 26.5 1.0 CA A:GLY359 4.9 24.2 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F) within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe417 b:33.3 occ:1.00 FE B:HEM417 0.0 33.3 1.0 NA B:HEM417 2.0 30.8 1.0 NC B:HEM417 2.1 32.0 1.0 NB B:HEM417 2.1 31.7 1.0 ND B:HEM417 2.1 31.7 1.0 SG B:CYS357 2.3 32.7 1.0 C4D B:HEM417 3.1 31.7 1.0 C4B B:HEM417 3.1 31.2 1.0 C4A B:HEM417 3.1 30.6 1.0 C1C B:HEM417 3.1 31.6 1.0 C1A B:HEM417 3.1 30.3 1.0 C4C B:HEM417 3.1 30.8 1.0 C1D B:HEM417 3.1 32.4 1.0 C1B B:HEM417 3.1 30.8 1.0 CB B:CYS357 3.2 30.3 1.0 CHC B:HEM417 3.4 32.1 1.0 CHA B:HEM417 3.5 30.3 1.0 CHD B:HEM417 3.5 31.5 1.0 CHB B:HEM417 3.5 29.5 1.0 CA B:CYS357 4.0 31.6 1.0 C2A B:HEM417 4.3 30.5 1.0 C3D B:HEM417 4.3 32.1 1.0 C2C B:HEM417 4.3 32.6 1.0 C3C B:HEM417 4.3 31.8 1.0 C3A B:HEM417 4.3 30.2 1.0 C3B B:HEM417 4.3 30.1 1.0 C2D B:HEM417 4.4 33.3 1.0 C2B B:HEM417 4.4 30.6 1.0 N B:GLY359 4.5 27.0 1.0 N B:LEU358 4.6 32.4 1.0 C B:CYS357 4.7 31.9 1.0 CA B:GLY359 4.9 27.4 1.0

Y.Liu, F.Jiang, Q.Guo, X.Chen, J.Jin, Y.Sun, Z.Rao. Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F) To Be Published.