Iron in PDB 1ea0: Alpha Subunit of A. Brasilense Glutamate Synthase

All present enzymatic activity of Alpha Subunit of A. Brasilense Glutamate Synthase:
1.4.1.13;

The structure of Alpha Subunit of A. Brasilense Glutamate Synthase, PDB code: 1ea0 was solved by C.Binda, R.T.Bossi, M.A.Vanoni, A.Mattevi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20. / 3.0
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	233.612, 233.612, 305.089, 90.00, 90.00, 120.00
R / Rfree (%)	25.587 / 28.712

The binding sites of Iron atom in the Alpha Subunit of A. Brasilense Glutamate Synthase (pdb code 1ea0). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 6 binding sites of Iron where determined in the Alpha Subunit of A. Brasilense Glutamate Synthase, PDB code: 1ea0:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6;

Iron binding site 1 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2476 b:3.4 occ:1.00 FE1 A:F3S2476 0.0 3.4 1.0 S1 A:F3S2476 2.1 26.8 1.0 SG A:CYS1102 2.1 41.7 1.0 S2 A:F3S2476 2.2 18.6 1.0 S3 A:F3S2476 2.2 16.3 1.0 FE4 A:F3S2476 2.4 3.0 1.0 FE3 A:F3S2476 2.8 8.7 1.0 CB A:CYS1102 2.9 43.7 1.0 CA A:CYS1102 3.6 46.9 1.0 S4 A:F3S2476 3.7 28.8 1.0 N A:MET1104 4.1 53.7 1.0 SG A:CYS1108 4.2 48.2 1.0 N A:ILE1103 4.2 48.1 1.0 C A:CYS1102 4.3 49.0 1.0 SG A:CYS1113 4.4 47.1 1.0 CA A:MET1104 4.6 55.0 1.0 CG1 A:VAL1117 4.6 55.4 1.0 CE A:MET479 4.7 37.3 1.0 CB A:VAL1117 4.7 52.2 1.0 SG A:CYS1118 4.9 54.7 1.0 N A:CYS1102 4.9 47.7 1.0

Iron binding site 2 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2476 b:8.7 occ:1.00 FE3 A:F3S2476 0.0 8.7 1.0 SG A:CYS1113 1.9 47.1 1.0 S3 A:F3S2476 2.0 16.3 1.0 S4 A:F3S2476 2.1 28.8 1.0 S1 A:F3S2476 2.2 26.8 1.0 FE4 A:F3S2476 2.7 3.0 1.0 FE1 A:F3S2476 2.8 3.4 1.0 S2 A:F3S2476 3.4 18.6 1.0 CB A:CYS1113 3.5 49.1 1.0 CA A:CYS1113 4.2 44.4 1.0 SG A:CYS1108 4.3 48.2 1.0 CD A:PRO1114 4.4 36.1 1.0 N A:CYS1118 4.5 48.0 1.0 CB A:VAL1117 4.6 52.2 1.0 SG A:CYS1102 4.6 41.7 1.0 CG1 A:VAL1105 4.7 45.5 1.0 N A:PRO1114 4.8 35.3 1.0 C A:CYS1113 4.9 41.6 1.0

Iron binding site 3 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2476 b:3.0 occ:1.00 FE4 A:F3S2476 0.0 3.0 1.0 SG A:CYS1108 1.9 48.2 1.0 S1 A:F3S2476 2.1 26.8 1.0 S4 A:F3S2476 2.2 28.8 1.0 S2 A:F3S2476 2.2 18.6 1.0 FE1 A:F3S2476 2.4 3.4 1.0 FE3 A:F3S2476 2.7 8.7 1.0 S3 A:F3S2476 3.3 16.3 1.0 CB A:CYS1108 3.4 39.0 1.0 SG A:CYS1118 3.6 54.7 1.0 CA A:CYS1108 4.1 48.5 1.0 N A:CYS1108 4.2 54.2 1.0 SG A:CYS1113 4.2 47.1 1.0 SG A:CYS1102 4.3 41.7 1.0 CE A:MET479 4.5 37.3 1.0 N A:GLN1107 4.5 52.9 1.0 C A:GLN1107 4.8 54.7 1.0 N A:CYS1118 4.9 48.0 1.0 N A:ARG1106 4.9 55.4 1.0

Iron binding site 4 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2476 b:1.0 occ:1.00 FE1 B:F3S2476 0.0 1.0 1.0 SG B:CYS1102 1.9 38.4 1.0 S3 B:F3S2476 2.1 27.5 1.0 S2 B:F3S2476 2.2 13.6 1.0 S1 B:F3S2476 2.2 26.1 1.0 FE3 B:F3S2476 2.5 1.6 1.0 FE4 B:F3S2476 2.6 2.5 1.0 CB B:CYS1102 2.9 45.9 1.0 CA B:CYS1102 3.6 50.1 1.0 S4 B:F3S2476 3.8 31.4 1.0 N B:ILE1103 4.1 48.2 1.0 SG B:CYS1113 4.3 47.1 1.0 C B:CYS1102 4.3 51.0 1.0 N B:MET1104 4.3 56.9 1.0 SG B:CYS1108 4.4 50.1 1.0 CE B:MET479 4.6 41.1 1.0 CG1 B:VAL1117 4.7 55.9 1.0 CA B:MET1104 4.9 54.6 1.0 N B:CYS1102 4.9 50.9 1.0 CB B:VAL1117 4.9 51.7 1.0

Iron binding site 5 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2476 b:1.6 occ:1.00 FE3 B:F3S2476 0.0 1.6 1.0 S4 B:F3S2476 2.1 31.4 1.0 SG B:CYS1113 2.1 47.1 1.0 S1 B:F3S2476 2.1 26.1 1.0 S3 B:F3S2476 2.2 27.5 1.0 FE4 B:F3S2476 2.5 2.5 1.0 FE1 B:F3S2476 2.5 1.0 1.0 S2 B:F3S2476 3.1 13.6 1.0 CB B:CYS1113 3.5 48.0 1.0 SG B:CYS1108 4.2 50.1 1.0 SG B:CYS1102 4.3 38.4 1.0 CA B:CYS1113 4.4 43.9 1.0 CD B:PRO1114 4.6 39.2 1.0 N B:CYS1118 4.6 47.9 1.0 CB B:VAL1117 4.9 51.7 1.0 CG1 B:VAL1105 5.0 50.6 1.0 CB B:CYS1118 5.0 55.2 1.0 C B:CYS1113 5.0 43.2 1.0

Iron binding site 6 out of 6 in the Alpha Subunit of A. Brasilense Glutamate Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Alpha Subunit of A. Brasilense Glutamate Synthase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2476 b:2.5 occ:1.00 FE4 B:F3S2476 0.0 2.5 1.0 SG B:CYS1108 1.9 50.1 1.0 S2 B:F3S2476 2.1 13.6 1.0 S4 B:F3S2476 2.1 31.4 1.0 S3 B:F3S2476 2.2 27.5 1.0 FE3 B:F3S2476 2.5 1.6 1.0 FE1 B:F3S2476 2.6 1.0 1.0 CB B:CYS1108 3.5 39.5 1.0 S1 B:F3S2476 3.6 26.1 1.0 SG B:CYS1118 4.0 55.9 1.0 N B:CYS1108 4.0 53.5 1.0 CA B:CYS1108 4.1 48.3 1.0 SG B:CYS1102 4.2 38.4 1.0 CB B:CYS1118 4.2 55.2 1.0 SG B:CYS1113 4.2 47.1 1.0 CE B:MET479 4.3 41.1 1.0 N B:GLN1107 4.4 54.6 1.0 C B:GLN1107 4.7 54.8 1.0 N B:ARG1106 4.9 56.5 1.0 CA B:ARG1106 5.0 54.0 1.0 N B:CYS1118 5.0 47.9 1.0

C.Binda, R.T.Bossi, S.Wakatsuki, S.Arzt, A.Coda, B.Curti, M.A.Vanoni, A.Mattevi. Cross-Talk and Ammonia Channeling Between Active Centers in the Unexpected Domain Arrangement of Glutamate Synthase Structure V. 8 1299 2000.
ISSN: ISSN 0969-2126
PubMed: 11188694
DOI: 10.1016/S0969-2126(00)00540-2