Iron in PDB 1fzh: Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas

All present enzymatic activity of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas:
1.14.13.25;

The structure of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas, PDB code: 1fzh was solved by D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.00 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.270, 174.620, 223.000, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 26

The structure of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas also contains other interesting chemical elements:

Xenon	(Xe)	11 atoms
Calcium	(Ca)	4 atoms

The binding sites of Iron atom in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas (pdb code 1fzh). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas, PDB code: 1fzh:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe5001 b:27.9 occ:1.00 O A:HOH9142 1.6 38.3 1.0 OE1 A:GLU114 2.0 30.6 1.0 ND1 A:HIS147 2.1 21.4 1.0 OE2 A:GLU144 2.5 23.2 1.0 O A:HOH9141 2.5 25.5 1.0 CE1 A:HIS147 3.0 22.9 1.0 CD A:GLU114 3.1 30.0 1.0 FE A:FE5002 3.1 31.6 1.0 CG A:HIS147 3.3 23.4 1.0 CD A:GLU144 3.3 23.9 1.0 OE1 A:GLU144 3.4 23.2 1.0 OE2 A:GLU114 3.5 32.5 1.0 CB A:HIS147 3.7 21.4 1.0 OE2 A:GLU243 4.0 45.9 1.0 NE2 A:HIS147 4.2 22.1 1.0 CE1 A:HIS246 4.3 33.9 1.0 CD2 A:HIS147 4.3 21.9 1.0 CG A:GLU114 4.4 30.0 1.0 ND1 A:HIS246 4.5 34.4 1.0 OE2 A:GLU209 4.5 40.8 1.0 CB A:GLU114 4.6 27.6 1.0 CG2 A:ILE239 4.7 33.2 1.0 CG A:GLU144 4.8 24.5 1.0 CA A:GLU114 4.8 26.6 1.0 CD A:GLU243 4.8 45.8 1.0 OE1 A:GLU243 4.8 45.3 1.0 CA A:GLU144 4.9 24.3 1.0

Iron binding site 2 out of 4 in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe5002 b:31.6 occ:1.00 O A:HOH9142 1.5 38.3 1.0 OE2 A:GLU209 1.9 40.8 1.0 ND1 A:HIS246 2.2 34.4 1.0 OE1 A:GLU243 2.5 45.3 1.0 OE1 A:GLU144 2.7 23.2 1.0 OE2 A:GLU243 2.8 45.9 1.0 CE1 A:HIS246 2.8 33.9 1.0 CD A:GLU243 3.0 45.8 1.0 CD A:GLU209 3.0 40.8 1.0 FE A:FE5001 3.1 27.9 1.0 CG A:HIS246 3.3 33.4 1.0 CD A:GLU144 3.7 23.9 1.0 NE2 A:GLN140 3.7 20.9 1.0 OE1 A:GLU209 3.8 41.3 1.0 OE2 A:GLU144 3.9 23.2 1.0 CB A:HIS246 3.9 35.3 1.0 CG A:GLU209 3.9 39.4 1.0 NE2 A:HIS246 4.0 33.3 1.0 O A:HOH9141 4.1 25.5 1.0 CD2 A:HIS246 4.3 33.4 1.0 CG A:GLU243 4.5 44.4 1.0 ND1 A:HIS147 4.5 21.4 1.0 CE1 A:HIS147 4.5 22.9 1.0 CD A:GLN140 4.7 22.5 1.0 CB A:GLU209 4.8 36.6 1.0 OE1 A:GLU114 4.9 30.6 1.0

Iron binding site 3 out of 4 in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe5003 b:28.6 occ:1.00 O B:HOH9145 1.9 25.3 1.0 OE1 B:GLU114 2.0 30.8 1.0 OE2 B:GLU144 2.2 28.6 1.0 O B:HOH9144 2.2 29.2 1.0 ND1 B:HIS147 2.3 28.4 1.0 CD B:GLU114 3.0 32.4 1.0 CD B:GLU144 3.0 28.0 1.0 FE B:FE5004 3.1 36.3 1.0 CE1 B:HIS147 3.1 27.6 1.0 OE1 B:GLU144 3.2 26.3 1.0 OE2 B:GLU114 3.3 31.2 1.0 CG B:HIS147 3.4 27.8 1.0 CB B:HIS147 3.8 25.9 1.0 OE2 B:GLU243 4.1 45.8 1.0 NE2 B:HIS147 4.3 26.5 1.0 CG B:GLU114 4.4 31.4 1.0 OE1 B:GLU209 4.4 38.5 1.0 ND1 B:HIS246 4.4 44.7 1.0 CD2 B:HIS147 4.5 28.3 1.0 CE1 B:HIS246 4.5 44.4 1.0 OE1 B:GLU243 4.5 41.4 1.0 CG B:GLU144 4.5 25.6 1.0 CA B:GLU144 4.7 25.8 1.0 CD B:GLU243 4.7 44.5 1.0 CB B:GLU114 4.7 31.1 1.0 CA B:GLU114 4.8 30.9 1.0 CG2 B:ILE239 4.9 32.4 1.0 CB B:GLU144 4.9 25.0 1.0

Iron binding site 4 out of 4 in the Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Methane Monooxygenase Hydroxylase, Form II Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe5004 b:36.3 occ:1.00 OE1 B:GLU209 1.8 38.5 1.0 O B:HOH9145 2.1 25.3 1.0 OE1 B:GLU243 2.3 41.4 1.0 ND1 B:HIS246 2.4 44.7 1.0 OE1 B:GLU144 2.4 26.3 1.0 CD B:GLU209 2.8 37.1 1.0 FE B:FE5003 3.1 28.6 1.0 CD B:GLU243 3.3 44.5 1.0 CE1 B:HIS246 3.3 44.4 1.0 CG B:HIS246 3.4 43.9 1.0 CD B:GLU144 3.4 28.0 1.0 OE2 B:GLU243 3.5 45.8 1.0 CG B:GLU209 3.6 37.6 1.0 CB B:HIS246 3.6 42.2 1.0 OE2 B:GLU209 3.7 36.3 1.0 OE2 B:GLU144 3.7 28.6 1.0 O B:HOH9144 3.9 29.2 1.0 NE2 B:GLN140 4.0 33.5 1.0 NE2 B:HIS246 4.5 45.2 1.0 CD2 B:HIS246 4.5 43.7 1.0 CD B:GLN140 4.6 33.1 1.0 CG B:GLU243 4.6 43.0 1.0 ND1 B:HIS147 4.7 28.4 1.0 CE1 B:HIS147 4.7 27.6 1.0 CB B:GLU209 4.8 37.8 1.0 CG B:GLU144 4.8 25.6 1.0 CG B:GLN140 4.8 32.8 1.0 OE1 B:GLU114 4.9 30.8 1.0

D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard. Xenon and Halogenated Alkanes Track Putative Substrate Binding Cavities in the Soluble Methane Monooxygenase Hydroxylase. Biochemistry V. 40 3476 2001.
ISSN: ISSN 0006-2960
PubMed: 11297413
DOI: 10.1021/BI0022487