Iron in PDB 1fzi: Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas

All present enzymatic activity of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas:
1.14.13.25;

The structure of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas, PDB code: 1fzi was solved by D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	12.00 / 3.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	61.700, 109.600, 330.200, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 25.3

The structure of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas also contains other interesting chemical elements:

Xenon

(Xe)

6 atoms

The binding sites of Iron atom in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas (pdb code 1fzi). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas, PDB code: 1fzi:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe5001 b:10.2 occ:1.00 OE1 A:GLU114 2.0 22.6 1.0 OE2 A:GLU144 2.0 19.9 1.0 ND1 A:HIS147 2.5 5.0 1.0 FE A:FE5002 2.8 10.2 1.0 CD A:GLU144 2.9 19.6 1.0 OE1 A:GLU144 3.1 19.5 1.0 CD A:GLU114 3.1 22.6 1.0 CE1 A:HIS147 3.3 4.5 1.0 OE2 A:GLU114 3.5 23.6 1.0 CG A:HIS147 3.6 5.1 1.0 OE2 A:GLU243 3.9 55.3 1.0 OE1 A:GLU243 3.9 47.8 1.0 CB A:HIS147 3.9 7.0 1.0 OE2 A:GLU209 4.2 60.3 1.0 CE1 A:HIS246 4.3 58.5 1.0 ND1 A:HIS246 4.3 58.3 1.0 CD A:GLU243 4.3 54.3 1.0 CG A:GLU144 4.4 16.6 1.0 CG A:GLU114 4.4 21.5 1.0 NE2 A:HIS147 4.5 5.4 1.0 CD2 A:HIS147 4.7 7.0 1.0 CA A:GLU114 4.7 6.4 1.0 CB A:GLU114 4.7 18.3 1.0 CA A:GLU144 4.8 0.0 1.0 CG2 A:ILE239 4.8 4.2 1.0 CB A:GLU144 4.9 16.6 1.0

Iron binding site 2 out of 4 in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe5002 b:10.2 occ:1.00 OE1 A:GLU243 2.0 47.8 1.0 OE2 A:GLU209 2.1 60.3 1.0 ND1 A:HIS246 2.1 58.3 1.0 OE1 A:GLU144 2.4 19.5 1.0 FE A:FE5001 2.8 10.2 1.0 CE1 A:HIS246 2.8 58.5 1.0 CD A:GLU209 3.0 61.5 1.0 CD A:GLU243 3.0 54.3 1.0 CG A:HIS246 3.3 59.4 1.0 CD A:GLU144 3.3 19.6 1.0 OE2 A:GLU243 3.4 55.3 1.0 OE2 A:GLU144 3.5 19.9 1.0 NE2 A:GLN140 3.6 19.4 1.0 CG A:GLU209 3.8 59.3 1.0 CB A:HIS246 3.8 60.1 1.0 OE1 A:GLU209 3.9 58.7 1.0 NE2 A:HIS246 4.1 57.6 1.0 CD2 A:HIS246 4.3 59.2 1.0 CG A:GLU243 4.4 52.8 1.0 ND1 A:HIS147 4.4 5.0 1.0 CE1 A:HIS147 4.5 4.5 1.0 CD A:GLN140 4.5 20.3 1.0 CG A:GLU144 4.7 16.6 1.0 OE1 A:GLU114 4.7 22.6 1.0 CG A:GLN140 4.8 20.5 1.0 CB A:GLU209 5.0 60.8 1.0

Iron binding site 3 out of 4 in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe5003 b:15.2 occ:1.00 OE1 B:GLU114 2.0 28.5 1.0 OE2 B:GLU144 2.1 11.8 1.0 ND1 B:HIS147 2.1 5.2 1.0 FE B:FE5004 2.7 15.2 1.0 CD B:GLU144 3.0 9.9 1.0 CE1 B:HIS147 3.0 5.9 1.0 CD B:GLU114 3.1 30.1 1.0 CG B:HIS147 3.2 4.9 1.0 OE1 B:GLU144 3.2 11.2 1.0 OE2 B:GLU114 3.6 34.8 1.0 CB B:HIS147 3.6 5.2 1.0 OE2 B:GLU243 4.0 45.6 1.0 CE1 B:HIS246 4.2 8.3 1.0 NE2 B:HIS147 4.2 6.9 1.0 CD2 B:HIS147 4.3 6.4 1.0 ND1 B:HIS246 4.3 11.7 1.0 CG B:GLU144 4.4 7.1 1.0 OE1 B:GLU243 4.4 37.7 1.0 CG B:GLU114 4.4 26.7 1.0 OE2 B:GLU209 4.5 0.0 1.0 CD B:GLU243 4.6 41.2 1.0 CA B:GLU144 4.6 19.3 1.0 CB B:GLU114 4.7 25.6 1.0 CA B:GLU114 4.7 13.3 1.0 CG2 B:ILE239 4.8 0.0 1.0 CB B:GLU144 4.8 5.2 1.0

Iron binding site 4 out of 4 in the Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Methane Monooxygenase Hydroxylase, Form I Pressurized with Xenon Gas within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe5004 b:15.2 occ:1.00 OE2 B:GLU209 2.2 0.0 1.0 ND1 B:HIS246 2.2 11.7 1.0 OE1 B:GLU144 2.2 11.2 1.0 OE1 B:GLU243 2.4 37.7 1.0 FE B:FE5003 2.7 15.2 1.0 CE1 B:HIS246 2.8 8.3 1.0 CD B:GLU144 3.1 9.9 1.0 OE2 B:GLU144 3.2 11.8 1.0 CD B:GLU243 3.3 41.2 1.0 CD B:GLU209 3.3 0.5 1.0 OE2 B:GLU243 3.4 45.6 1.0 CG B:HIS246 3.4 10.8 1.0 NE2 B:GLN140 3.7 28.7 1.0 NE2 B:HIS246 4.1 13.6 1.0 CB B:HIS246 4.1 11.8 1.0 ND1 B:HIS147 4.1 5.2 1.0 CG B:GLU209 4.2 0.4 1.0 OE1 B:GLU209 4.2 0.7 1.0 CE1 B:HIS147 4.3 5.9 1.0 CD2 B:HIS246 4.4 12.3 1.0 OE1 B:GLU114 4.4 28.5 1.0 CG B:GLU144 4.5 7.1 1.0 CD B:GLN140 4.6 29.1 1.0 CG B:GLU243 4.7 38.4 1.0 CG B:GLN140 4.8 29.3 1.0

D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard. Xenon and Halogenated Alkanes Track Putative Substrate Binding Cavities in the Soluble Methane Monooxygenase Hydroxylase. Biochemistry V. 40 3476 2001.
ISSN: ISSN 0006-2960
PubMed: 11297413
DOI: 10.1021/BI0022487