Iron in PDB 1g1m: All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii

All present enzymatic activity of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii:
1.18.6.1;

The structure of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii, PDB code: 1g1m was solved by P.Strop, P.M.Takahara, H.-J.Chiu, H.C.Angove, B.K.Burgess, D.C.Rees, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.00 / 2.25
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.000, 93.300, 62.800, 90.00, 98.90, 90.00
R / Rfree (%)	23.8 / 28.8

The binding sites of Iron atom in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii (pdb code 1g1m). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii, PDB code: 1g1m:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:34.4 occ:1.00 FE1 A:SF4501 0.0 34.4 1.0 S4 A:SF4501 2.3 44.2 1.0 S3 A:SF4501 2.3 42.3 1.0 SG B:CYS97 2.3 44.6 1.0 S2 A:SF4501 2.3 43.8 1.0 FE4 A:SF4501 2.6 30.3 1.0 FE2 A:SF4501 2.6 32.3 1.0 FE3 A:SF4501 2.8 33.8 1.0 CB B:CYS97 3.3 39.2 1.0 CA B:CYS97 3.7 41.5 1.0 S1 A:SF4501 3.9 45.8 1.0 CA B:GLY134 4.0 32.9 1.0 N B:ALA98 4.0 41.9 1.0 C B:CYS97 4.2 41.6 1.0 N B:GLY134 4.5 37.8 1.0 SG B:CYS132 4.5 41.1 1.0 N B:GLY99 4.6 45.4 1.0 SG A:CYS97 4.8 45.4 1.0 SG A:CYS132 4.8 43.1 1.0

Iron binding site 2 out of 4 in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:32.3 occ:1.00 FE2 A:SF4501 0.0 32.3 1.0 S4 A:SF4501 2.3 44.2 1.0 S1 A:SF4501 2.3 45.8 1.0 SG A:CYS132 2.3 43.1 1.0 S3 A:SF4501 2.4 42.3 1.0 FE4 A:SF4501 2.5 30.3 1.0 FE1 A:SF4501 2.6 34.4 1.0 FE3 A:SF4501 2.7 33.8 1.0 CB A:CYS132 3.3 25.1 1.0 O B:HOH293 3.8 45.0 1.0 S2 A:SF4501 3.8 43.8 1.0 N A:GLY134 4.0 26.6 1.0 CA A:GLY134 4.1 28.0 1.0 SG B:CYS132 4.6 41.1 1.0 CA A:CYS132 4.6 30.2 1.0 SG B:CYS97 4.7 44.6 1.0 SG A:CYS97 4.8 45.4 1.0 C A:CYS132 4.8 35.3 1.0 CA B:CYS97 4.8 41.5 1.0 N A:PHE135 4.9 26.5 1.0 N B:ALA98 4.9 41.9 1.0 N A:GLY133 4.9 31.7 1.0 C A:GLY134 5.0 30.6 1.0

Iron binding site 3 out of 4 in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:33.8 occ:1.00 FE3 A:SF4501 0.0 33.8 1.0 SG A:CYS97 2.3 45.4 1.0 S2 A:SF4501 2.3 43.8 1.0 S1 A:SF4501 2.3 45.8 1.0 S4 A:SF4501 2.4 44.2 1.0 FE4 A:SF4501 2.7 30.3 1.0 FE2 A:SF4501 2.7 32.3 1.0 FE1 A:SF4501 2.8 34.4 1.0 CB A:CYS97 3.4 32.6 1.0 CA A:CYS97 3.7 36.7 1.0 N A:ALA98 3.9 40.8 1.0 S3 A:SF4501 4.1 42.3 1.0 CA A:GLY134 4.1 28.0 1.0 C A:CYS97 4.2 38.9 1.0 N A:GLY134 4.5 26.6 1.0 SG A:CYS132 4.7 43.1 1.0 N A:GLY99 4.7 33.4 1.0 SG B:CYS132 4.8 41.1 1.0 SG B:CYS97 4.8 44.6 1.0 O A:GLY96 4.9 40.0 1.0

Iron binding site 4 out of 4 in the All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of All-Ferrous Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:30.3 occ:1.00 FE4 A:SF4501 0.0 30.3 1.0 S1 A:SF4501 2.3 45.8 1.0 SG B:CYS132 2.3 41.1 1.0 S2 A:SF4501 2.3 43.8 1.0 S3 A:SF4501 2.4 42.3 1.0 FE2 A:SF4501 2.5 32.3 1.0 FE1 A:SF4501 2.6 34.4 1.0 FE3 A:SF4501 2.7 33.8 1.0 CB B:CYS132 3.3 31.1 1.0 S4 A:SF4501 3.8 44.2 1.0 N B:GLY134 3.9 37.8 1.0 CA B:GLY134 3.9 32.9 1.0 O B:HOH293 4.5 45.0 1.0 SG A:CYS132 4.6 43.1 1.0 CA B:CYS132 4.6 32.0 1.0 SG B:CYS97 4.7 44.6 1.0 SG A:CYS97 4.8 45.4 1.0 N B:PHE135 4.8 35.0 1.0 C B:CYS132 4.8 37.5 1.0 O A:GLY96 4.9 40.0 1.0 N B:GLY133 4.9 39.9 1.0 C B:GLY134 4.9 40.6 1.0 CD2 B:PHE135 5.0 37.2 1.0

P.Strop, P.M.Takahara, H.Chiu, H.C.Angove, B.K.Burgess, D.C.Rees. Crystal Structure of the All-Ferrous [4FE-4S]0 Form of the Nitrogenase Iron Protein From Azotobacter Vinelandii. Biochemistry V. 40 651 2001.
ISSN: ISSN 0006-2960
PubMed: 11170381
DOI: 10.1021/BI0016467