Iron in PDB 1hse: H253M N Terminal Lobe of Human Lactoferrin

The structure of H253M N Terminal Lobe of Human Lactoferrin, PDB code: 1hse was solved by H.Nicholson, B.F.Anderson, E.N.Baker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.20
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	58.400, 58.400, 217.900, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / n/a

The binding sites of Iron atom in the H253M N Terminal Lobe of Human Lactoferrin (pdb code 1hse). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the H253M N Terminal Lobe of Human Lactoferrin, PDB code: 1hse:

Iron binding site 1 out of 1 in the H253M N Terminal Lobe of Human Lactoferrin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of H253M N Terminal Lobe of Human Lactoferrin within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe400 b:28.1 occ:1.00 OH A:TYR192 1.9 24.5 1.0 OD1 A:ASP60 2.0 14.1 1.0 OH A:TYR92 2.1 35.8 1.0 O1 A:CO3401 2.1 17.2 1.0 O2 A:CO3401 2.2 17.6 1.0 C A:CO3401 2.5 13.6 1.0 CZ A:TYR192 2.9 28.8 1.0 CG A:ASP60 2.9 11.3 1.0 CZ A:TYR92 3.2 23.6 1.0 OD2 A:ASP60 3.4 16.6 1.0 CE2 A:TYR92 3.7 14.0 1.0 SD A:MET253 3.7 22.7 1.0 CE1 A:TYR192 3.7 19.5 1.0 CE2 A:TYR192 3.8 19.1 1.0 O3 A:CO3401 3.8 15.2 1.0 O A:HOH606 3.8 29.9 1.0 CG A:MET253 4.1 19.2 1.0 CB A:ASP60 4.2 14.4 1.0 CE1 A:TYR92 4.3 14.4 1.0 NH2 A:ARG210 4.4 18.6 1.0 NH2 A:ARG121 4.5 11.6 1.0 NE A:ARG121 4.5 38.5 1.0 N A:ALA123 4.8 12.7 1.0 CA A:ASP60 4.8 16.8 1.0 CB A:THR122 4.8 29.0 1.0 N A:THR122 4.8 13.4 1.0 CD1 A:TYR192 4.9 16.5 1.0 CD2 A:TYR192 5.0 17.0 1.0 CD2 A:TYR92 5.0 15.9 1.0

H.Nicholson, B.F.Anderson, T.Bland, S.C.Shewry, J.W.Tweedie, E.N.Baker. Mutagenesis of the Histidine Ligand in Human Lactoferrin: Iron Binding Properties and Crystal Structure of the Histidine-253-->Methionine Mutant. Biochemistry V. 36 341 1997.
ISSN: ISSN 0006-2960
PubMed: 9003186
DOI: 10.1021/BI961908Y