Iron in PDB 1lj1: Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
Enzymatic activity of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
All present enzymatic activity of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3:
1.3.99.1;
Protein crystallography data
The structure of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3, PDB code: 1lj1
was solved by
C.G.Mowat,
K.L.Pankhurst,
C.S.Miles,
D.Leys,
M.D.Walkinshaw,
G.A.Reid,
S.K.Chapman,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
15.00 /
2.00
|
|
Space group
|
P 1 21 1
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
77.971,
88.280,
90.087,
90.00,
103.89,
90.00
|
|
R / Rfree (%)
|
16.1 /
23.5
|
Other elements in 1lj1:
The structure of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
(pdb code 1lj1). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the
Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3, PDB code: 1lj1:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Iron binding site 1 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 1 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe801
b:15.1
occ:1.00
|
FE
|
A:HEM801
|
0.0
|
15.1
|
1.0
|
|
NC
|
A:HEM801
|
2.0
|
15.2
|
1.0
|
|
NA
|
A:HEM801
|
2.0
|
15.8
|
1.0
|
|
NE2
|
A:HIS18
|
2.0
|
13.7
|
1.0
|
|
NB
|
A:HEM801
|
2.0
|
17.7
|
1.0
|
|
ND
|
A:HEM801
|
2.0
|
15.7
|
1.0
|
|
NE2
|
A:HIS75
|
2.1
|
17.6
|
1.0
|
|
CE1
|
A:HIS18
|
2.9
|
13.5
|
1.0
|
|
C1C
|
A:HEM801
|
3.0
|
17.0
|
1.0
|
|
C4B
|
A:HEM801
|
3.0
|
17.3
|
1.0
|
|
C1A
|
A:HEM801
|
3.0
|
17.1
|
1.0
|
|
C4C
|
A:HEM801
|
3.0
|
15.9
|
1.0
|
|
C4A
|
A:HEM801
|
3.1
|
17.3
|
1.0
|
|
C1D
|
A:HEM801
|
3.1
|
14.5
|
1.0
|
|
C1B
|
A:HEM801
|
3.1
|
16.5
|
1.0
|
|
C4D
|
A:HEM801
|
3.1
|
14.1
|
1.0
|
|
CD2
|
A:HIS75
|
3.1
|
18.0
|
1.0
|
|
CE1
|
A:HIS75
|
3.1
|
17.2
|
1.0
|
|
CD2
|
A:HIS18
|
3.2
|
11.1
|
1.0
|
|
CHA
|
A:HEM801
|
3.4
|
15.2
|
1.0
|
|
CHC
|
A:HEM801
|
3.4
|
17.5
|
1.0
|
|
CHD
|
A:HEM801
|
3.4
|
13.2
|
1.0
|
|
CHB
|
A:HEM801
|
3.5
|
16.2
|
1.0
|
|
ND1
|
A:HIS18
|
4.1
|
12.5
|
1.0
|
|
CG
|
A:HIS18
|
4.2
|
13.6
|
1.0
|
|
ND1
|
A:HIS75
|
4.2
|
19.2
|
1.0
|
|
C3C
|
A:HEM801
|
4.2
|
16.4
|
1.0
|
|
C2C
|
A:HEM801
|
4.2
|
16.9
|
1.0
|
|
C3B
|
A:HEM801
|
4.2
|
18.2
|
1.0
|
|
C2A
|
A:HEM801
|
4.3
|
18.2
|
1.0
|
|
CG
|
A:HIS75
|
4.3
|
18.2
|
1.0
|
|
C3A
|
A:HEM801
|
4.3
|
17.0
|
1.0
|
|
C2B
|
A:HEM801
|
4.3
|
16.7
|
1.0
|
|
C3D
|
A:HEM801
|
4.3
|
14.5
|
1.0
|
|
C2D
|
A:HEM801
|
4.3
|
15.7
|
1.0
|
|
O
|
A:HOH2095
|
4.8
|
26.7
|
1.0
|
|
Iron binding site 2 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 2 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe802
b:17.7
occ:1.00
|
FE
|
A:HEM802
|
0.0
|
17.7
|
1.0
|
|
NC
|
A:HEM802
|
2.0
|
16.1
|
1.0
|
|
NB
|
A:HEM802
|
2.0
|
15.2
|
1.0
|
|
NA
|
A:HEM802
|
2.0
|
19.9
|
1.0
|
|
ND
|
A:HEM802
|
2.0
|
20.4
|
1.0
|
|
NE2
|
A:HIS40
|
2.1
|
18.1
|
1.0
|
|
NE2
|
A:HIS8
|
2.1
|
15.4
|
1.0
|
|
C4B
|
A:HEM802
|
3.0
|
16.2
|
1.0
|
|
C4C
|
A:HEM802
|
3.0
|
18.8
|
1.0
|
|
C1C
|
A:HEM802
|
3.0
|
16.2
|
1.0
|
|
CE1
|
A:HIS40
|
3.0
|
17.9
|
1.0
|
|
C1D
|
A:HEM802
|
3.0
|
19.9
|
1.0
|
|
C1B
|
A:HEM802
|
3.0
|
17.1
|
1.0
|
|
C1A
|
A:HEM802
|
3.0
|
19.8
|
1.0
|
|
C4D
|
A:HEM802
|
3.1
|
21.8
|
1.0
|
|
C4A
|
A:HEM802
|
3.1
|
20.3
|
1.0
|
|
CD2
|
A:HIS40
|
3.1
|
17.6
|
1.0
|
|
CD2
|
A:HIS8
|
3.1
|
16.5
|
1.0
|
|
CE1
|
A:HIS8
|
3.2
|
16.0
|
1.0
|
|
CHC
|
A:HEM802
|
3.3
|
17.3
|
1.0
|
|
CHD
|
A:HEM802
|
3.4
|
19.5
|
1.0
|
|
CHA
|
A:HEM802
|
3.4
|
20.7
|
1.0
|
|
CHB
|
A:HEM802
|
3.4
|
18.7
|
1.0
|
|
ND1
|
A:HIS40
|
4.2
|
16.6
|
1.0
|
|
CG
|
A:HIS40
|
4.2
|
17.3
|
1.0
|
|
C3B
|
A:HEM802
|
4.2
|
16.6
|
1.0
|
|
C2B
|
A:HEM802
|
4.2
|
18.3
|
1.0
|
|
C3C
|
A:HEM802
|
4.2
|
17.5
|
1.0
|
|
C2C
|
A:HEM802
|
4.3
|
16.3
|
1.0
|
|
C2D
|
A:HEM802
|
4.3
|
23.3
|
1.0
|
|
C2A
|
A:HEM802
|
4.3
|
19.5
|
1.0
|
|
CG
|
A:HIS8
|
4.3
|
16.4
|
1.0
|
|
C3A
|
A:HEM802
|
4.3
|
20.1
|
1.0
|
|
C3D
|
A:HEM802
|
4.3
|
23.6
|
1.0
|
|
ND1
|
A:HIS8
|
4.3
|
15.0
|
1.0
|
|
Iron binding site 3 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 3 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe803
b:16.6
occ:1.00
|
FE
|
A:HEM803
|
0.0
|
16.6
|
1.0
|
|
NB
|
A:HEM803
|
2.0
|
17.0
|
1.0
|
|
ND
|
A:HEM803
|
2.0
|
16.5
|
1.0
|
|
NC
|
A:HEM803
|
2.0
|
16.5
|
1.0
|
|
NA
|
A:HEM803
|
2.0
|
18.4
|
1.0
|
|
NE2
|
A:HIS58
|
2.1
|
13.9
|
1.0
|
|
NE2
|
A:HIS72
|
2.1
|
15.5
|
1.0
|
|
CE1
|
A:HIS58
|
3.0
|
15.3
|
1.0
|
|
C4B
|
A:HEM803
|
3.0
|
17.4
|
1.0
|
|
C1B
|
A:HEM803
|
3.0
|
17.2
|
1.0
|
|
C4C
|
A:HEM803
|
3.0
|
15.8
|
1.0
|
|
C1D
|
A:HEM803
|
3.0
|
16.9
|
1.0
|
|
C1C
|
A:HEM803
|
3.0
|
14.4
|
1.0
|
|
C4D
|
A:HEM803
|
3.0
|
19.2
|
1.0
|
|
C4A
|
A:HEM803
|
3.1
|
18.5
|
1.0
|
|
C1A
|
A:HEM803
|
3.1
|
18.9
|
1.0
|
|
CE1
|
A:HIS72
|
3.1
|
13.8
|
1.0
|
|
CD2
|
A:HIS72
|
3.2
|
15.3
|
1.0
|
|
CD2
|
A:HIS58
|
3.2
|
15.1
|
1.0
|
|
CHD
|
A:HEM803
|
3.4
|
16.0
|
1.0
|
|
CHC
|
A:HEM803
|
3.4
|
15.9
|
1.0
|
|
CHB
|
A:HEM803
|
3.4
|
16.8
|
1.0
|
|
CHA
|
A:HEM803
|
3.4
|
18.8
|
1.0
|
|
ND1
|
A:HIS58
|
4.1
|
14.1
|
1.0
|
|
C3B
|
A:HEM803
|
4.2
|
18.4
|
1.0
|
|
C2B
|
A:HEM803
|
4.2
|
17.5
|
1.0
|
|
ND1
|
A:HIS72
|
4.2
|
14.8
|
1.0
|
|
C3C
|
A:HEM803
|
4.3
|
17.3
|
1.0
|
|
C2C
|
A:HEM803
|
4.3
|
17.1
|
1.0
|
|
C3D
|
A:HEM803
|
4.3
|
19.5
|
1.0
|
|
C2D
|
A:HEM803
|
4.3
|
19.3
|
1.0
|
|
C2A
|
A:HEM803
|
4.3
|
20.1
|
1.0
|
|
CG
|
A:HIS58
|
4.3
|
14.3
|
1.0
|
|
C3A
|
A:HEM803
|
4.3
|
19.7
|
1.0
|
|
CG
|
A:HIS72
|
4.3
|
15.1
|
1.0
|
|
CG1
|
A:VAL46
|
5.0
|
20.5
|
1.0
|
|
Iron binding site 4 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 4 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe804
b:15.0
occ:1.00
|
FE
|
A:HEM804
|
0.0
|
15.0
|
1.0
|
|
NA
|
A:HEM804
|
2.0
|
16.5
|
1.0
|
|
NC
|
A:HEM804
|
2.0
|
15.7
|
1.0
|
|
ND
|
A:HEM804
|
2.0
|
14.4
|
1.0
|
|
NB
|
A:HEM804
|
2.0
|
17.4
|
1.0
|
|
NE2
|
A:HIS61
|
2.1
|
12.6
|
1.0
|
|
NE2
|
A:HIS86
|
2.1
|
15.5
|
1.0
|
|
C4B
|
A:HEM804
|
3.0
|
17.9
|
1.0
|
|
C4A
|
A:HEM804
|
3.0
|
16.8
|
1.0
|
|
C1C
|
A:HEM804
|
3.0
|
17.1
|
1.0
|
|
C1B
|
A:HEM804
|
3.0
|
17.6
|
1.0
|
|
C4C
|
A:HEM804
|
3.0
|
15.4
|
1.0
|
|
C1D
|
A:HEM804
|
3.0
|
14.8
|
1.0
|
|
CE1
|
A:HIS61
|
3.0
|
11.6
|
1.0
|
|
C1A
|
A:HEM804
|
3.0
|
17.1
|
1.0
|
|
CE1
|
A:HIS86
|
3.0
|
17.4
|
1.0
|
|
C4D
|
A:HEM804
|
3.1
|
13.1
|
1.0
|
|
CD2
|
A:HIS86
|
3.1
|
16.0
|
1.0
|
|
CD2
|
A:HIS61
|
3.1
|
12.9
|
1.0
|
|
CHC
|
A:HEM804
|
3.4
|
17.1
|
1.0
|
|
CHD
|
A:HEM804
|
3.4
|
14.6
|
1.0
|
|
CHB
|
A:HEM804
|
3.4
|
17.8
|
1.0
|
|
CHA
|
A:HEM804
|
3.4
|
14.3
|
1.0
|
|
ND1
|
A:HIS86
|
4.2
|
17.5
|
1.0
|
|
ND1
|
A:HIS61
|
4.2
|
11.5
|
1.0
|
|
C3B
|
A:HEM804
|
4.2
|
18.4
|
1.0
|
|
C2B
|
A:HEM804
|
4.2
|
18.9
|
1.0
|
|
CG
|
A:HIS86
|
4.2
|
16.2
|
1.0
|
|
CG
|
A:HIS61
|
4.2
|
11.9
|
1.0
|
|
C3A
|
A:HEM804
|
4.2
|
17.7
|
1.0
|
|
C2A
|
A:HEM804
|
4.3
|
17.1
|
1.0
|
|
C2C
|
A:HEM804
|
4.3
|
17.1
|
1.0
|
|
C3C
|
A:HEM804
|
4.3
|
17.8
|
1.0
|
|
C3D
|
A:HEM804
|
4.3
|
14.7
|
1.0
|
|
C2D
|
A:HEM804
|
4.3
|
13.8
|
1.0
|
|
Iron binding site 5 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 5 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe801
b:14.8
occ:1.00
|
FE
|
B:HEM801
|
0.0
|
14.8
|
1.0
|
|
NA
|
B:HEM801
|
2.0
|
16.3
|
1.0
|
|
NB
|
B:HEM801
|
2.0
|
15.4
|
1.0
|
|
ND
|
B:HEM801
|
2.0
|
15.1
|
1.0
|
|
NC
|
B:HEM801
|
2.0
|
15.8
|
1.0
|
|
NE2
|
B:HIS18
|
2.1
|
13.0
|
1.0
|
|
NE2
|
B:HIS75
|
2.2
|
17.1
|
1.0
|
|
CE1
|
B:HIS18
|
3.0
|
13.5
|
1.0
|
|
C1A
|
B:HEM801
|
3.0
|
17.5
|
1.0
|
|
C4B
|
B:HEM801
|
3.0
|
14.7
|
1.0
|
|
C4A
|
B:HEM801
|
3.0
|
18.8
|
1.0
|
|
C1B
|
B:HEM801
|
3.0
|
16.7
|
1.0
|
|
C1D
|
B:HEM801
|
3.1
|
14.8
|
1.0
|
|
C4C
|
B:HEM801
|
3.1
|
15.6
|
1.0
|
|
C4D
|
B:HEM801
|
3.1
|
13.2
|
1.0
|
|
C1C
|
B:HEM801
|
3.1
|
16.1
|
1.0
|
|
CE1
|
B:HIS75
|
3.1
|
16.2
|
1.0
|
|
CD2
|
B:HIS18
|
3.2
|
12.1
|
1.0
|
|
CD2
|
B:HIS75
|
3.2
|
16.3
|
1.0
|
|
CHA
|
B:HEM801
|
3.4
|
15.4
|
1.0
|
|
CHB
|
B:HEM801
|
3.4
|
16.9
|
1.0
|
|
CHD
|
B:HEM801
|
3.4
|
13.8
|
1.0
|
|
CHC
|
B:HEM801
|
3.4
|
15.9
|
1.0
|
|
ND1
|
B:HIS18
|
4.2
|
13.7
|
1.0
|
|
ND1
|
B:HIS75
|
4.2
|
15.3
|
1.0
|
|
C3B
|
B:HEM801
|
4.2
|
17.4
|
1.0
|
|
C2A
|
B:HEM801
|
4.2
|
18.9
|
1.0
|
|
C3A
|
B:HEM801
|
4.2
|
19.4
|
1.0
|
|
C2B
|
B:HEM801
|
4.3
|
18.6
|
1.0
|
|
C3C
|
B:HEM801
|
4.3
|
16.1
|
1.0
|
|
C2C
|
B:HEM801
|
4.3
|
16.8
|
1.0
|
|
C3D
|
B:HEM801
|
4.3
|
13.2
|
1.0
|
|
CG
|
B:HIS18
|
4.3
|
14.0
|
1.0
|
|
C2D
|
B:HEM801
|
4.3
|
14.3
|
1.0
|
|
CG
|
B:HIS75
|
4.3
|
15.7
|
1.0
|
|
Iron binding site 6 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 6 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe802
b:17.2
occ:1.00
|
FE
|
B:HEM802
|
0.0
|
17.2
|
1.0
|
|
NC
|
B:HEM802
|
2.0
|
16.5
|
1.0
|
|
NB
|
B:HEM802
|
2.0
|
16.9
|
1.0
|
|
NA
|
B:HEM802
|
2.0
|
17.4
|
1.0
|
|
ND
|
B:HEM802
|
2.0
|
18.9
|
1.0
|
|
NE2
|
B:HIS8
|
2.1
|
18.9
|
1.0
|
|
NE2
|
B:HIS40
|
2.1
|
14.7
|
1.0
|
|
C4B
|
B:HEM802
|
3.0
|
16.9
|
1.0
|
|
CD2
|
B:HIS8
|
3.0
|
15.8
|
1.0
|
|
C4C
|
B:HEM802
|
3.0
|
15.9
|
1.0
|
|
C1D
|
B:HEM802
|
3.0
|
19.9
|
1.0
|
|
C1B
|
B:HEM802
|
3.0
|
16.6
|
1.0
|
|
C1A
|
B:HEM802
|
3.0
|
19.7
|
1.0
|
|
C1C
|
B:HEM802
|
3.0
|
15.9
|
1.0
|
|
C4A
|
B:HEM802
|
3.1
|
17.7
|
1.0
|
|
C4D
|
B:HEM802
|
3.1
|
20.4
|
1.0
|
|
CE1
|
B:HIS40
|
3.1
|
15.4
|
1.0
|
|
CE1
|
B:HIS8
|
3.2
|
18.4
|
1.0
|
|
CD2
|
B:HIS40
|
3.2
|
15.2
|
1.0
|
|
CHD
|
B:HEM802
|
3.4
|
17.1
|
1.0
|
|
CHC
|
B:HEM802
|
3.4
|
15.8
|
1.0
|
|
CHB
|
B:HEM802
|
3.4
|
17.3
|
1.0
|
|
CHA
|
B:HEM802
|
3.4
|
20.1
|
1.0
|
|
CG
|
B:HIS8
|
4.2
|
17.8
|
1.0
|
|
C3B
|
B:HEM802
|
4.2
|
17.4
|
1.0
|
|
ND1
|
B:HIS40
|
4.2
|
15.5
|
1.0
|
|
C3C
|
B:HEM802
|
4.2
|
16.0
|
1.0
|
|
C2B
|
B:HEM802
|
4.2
|
17.0
|
1.0
|
|
ND1
|
B:HIS8
|
4.2
|
17.8
|
1.0
|
|
C2D
|
B:HEM802
|
4.3
|
20.7
|
1.0
|
|
C2C
|
B:HEM802
|
4.3
|
15.4
|
1.0
|
|
C2A
|
B:HEM802
|
4.3
|
18.2
|
1.0
|
|
C3A
|
B:HEM802
|
4.3
|
18.2
|
1.0
|
|
C3D
|
B:HEM802
|
4.3
|
22.1
|
1.0
|
|
CG
|
B:HIS40
|
4.3
|
14.9
|
1.0
|
|
Iron binding site 7 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 7 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe803
b:16.1
occ:1.00
|
FE
|
B:HEM803
|
0.0
|
16.1
|
1.0
|
|
NA
|
B:HEM803
|
2.0
|
17.6
|
1.0
|
|
NB
|
B:HEM803
|
2.0
|
16.6
|
1.0
|
|
ND
|
B:HEM803
|
2.0
|
16.2
|
1.0
|
|
NC
|
B:HEM803
|
2.0
|
14.7
|
1.0
|
|
NE2
|
B:HIS72
|
2.1
|
12.8
|
1.0
|
|
NE2
|
B:HIS58
|
2.2
|
12.6
|
1.0
|
|
CE1
|
B:HIS72
|
3.0
|
11.2
|
1.0
|
|
C1A
|
B:HEM803
|
3.0
|
18.0
|
1.0
|
|
C1C
|
B:HEM803
|
3.0
|
14.0
|
1.0
|
|
C4B
|
B:HEM803
|
3.0
|
16.8
|
1.0
|
|
C4C
|
B:HEM803
|
3.0
|
13.9
|
1.0
|
|
C4D
|
B:HEM803
|
3.0
|
17.0
|
1.0
|
|
C1B
|
B:HEM803
|
3.0
|
16.7
|
1.0
|
|
C4A
|
B:HEM803
|
3.1
|
17.5
|
1.0
|
|
C1D
|
B:HEM803
|
3.1
|
16.4
|
1.0
|
|
CD2
|
B:HIS58
|
3.1
|
10.9
|
1.0
|
|
CD2
|
B:HIS72
|
3.2
|
10.3
|
1.0
|
|
CE1
|
B:HIS58
|
3.3
|
12.5
|
1.0
|
|
CHC
|
B:HEM803
|
3.4
|
14.2
|
1.0
|
|
CHA
|
B:HEM803
|
3.4
|
17.3
|
1.0
|
|
CHB
|
B:HEM803
|
3.4
|
16.4
|
1.0
|
|
CHD
|
B:HEM803
|
3.4
|
16.0
|
1.0
|
|
ND1
|
B:HIS72
|
4.1
|
8.6
|
1.0
|
|
C2A
|
B:HEM803
|
4.2
|
19.3
|
1.0
|
|
C3B
|
B:HEM803
|
4.2
|
18.1
|
1.0
|
|
C2B
|
B:HEM803
|
4.2
|
19.0
|
1.0
|
|
C2C
|
B:HEM803
|
4.3
|
13.0
|
1.0
|
|
CG
|
B:HIS72
|
4.3
|
10.7
|
1.0
|
|
C3C
|
B:HEM803
|
4.3
|
14.6
|
1.0
|
|
C3A
|
B:HEM803
|
4.3
|
17.5
|
1.0
|
|
C3D
|
B:HEM803
|
4.3
|
17.4
|
1.0
|
|
CG
|
B:HIS58
|
4.3
|
12.2
|
1.0
|
|
C2D
|
B:HEM803
|
4.3
|
16.0
|
1.0
|
|
ND1
|
B:HIS58
|
4.3
|
11.4
|
1.0
|
|
CG1
|
B:VAL46
|
5.0
|
12.9
|
1.0
|
|
Iron binding site 8 out
of 8 in 1lj1
Go back to
Iron Binding Sites List in 1lj1
Iron binding site 8 out
of 8 in the Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3
 Mono view
 Stereo pair view
|
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of Crystal Structure of Q363F/R402A Mutant Flavocytochrome C3 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe804
b:13.8
occ:1.00
|
FE
|
B:HEM804
|
0.0
|
13.8
|
1.0
|
|
ND
|
B:HEM804
|
2.0
|
12.4
|
1.0
|
|
NC
|
B:HEM804
|
2.0
|
13.4
|
1.0
|
|
NA
|
B:HEM804
|
2.0
|
13.4
|
1.0
|
|
NB
|
B:HEM804
|
2.0
|
14.9
|
1.0
|
|
NE2
|
B:HIS86
|
2.1
|
14.1
|
1.0
|
|
NE2
|
B:HIS61
|
2.1
|
14.1
|
1.0
|
|
CE1
|
B:HIS86
|
2.9
|
15.2
|
1.0
|
|
C1D
|
B:HEM804
|
3.0
|
13.5
|
1.0
|
|
C4C
|
B:HEM804
|
3.0
|
13.5
|
1.0
|
|
C4A
|
B:HEM804
|
3.0
|
15.2
|
1.0
|
|
C4D
|
B:HEM804
|
3.0
|
12.4
|
1.0
|
|
C4B
|
B:HEM804
|
3.0
|
13.7
|
1.0
|
|
C1A
|
B:HEM804
|
3.1
|
14.0
|
1.0
|
|
C1B
|
B:HEM804
|
3.1
|
14.5
|
1.0
|
|
C1C
|
B:HEM804
|
3.1
|
15.4
|
1.0
|
|
CD2
|
B:HIS61
|
3.1
|
14.8
|
1.0
|
|
CE1
|
B:HIS61
|
3.1
|
11.9
|
1.0
|
|
CD2
|
B:HIS86
|
3.2
|
13.2
|
1.0
|
|
CHD
|
B:HEM804
|
3.4
|
13.5
|
1.0
|
|
CHA
|
B:HEM804
|
3.4
|
13.1
|
1.0
|
|
CHB
|
B:HEM804
|
3.4
|
13.8
|
1.0
|
|
CHC
|
B:HEM804
|
3.4
|
13.7
|
1.0
|
|
ND1
|
B:HIS86
|
4.1
|
11.8
|
1.0
|
|
ND1
|
B:HIS61
|
4.2
|
12.7
|
1.0
|
|
CG
|
B:HIS61
|
4.2
|
13.4
|
1.0
|
|
CG
|
B:HIS86
|
4.2
|
12.1
|
1.0
|
|
C3D
|
B:HEM804
|
4.3
|
12.1
|
1.0
|
|
C3B
|
B:HEM804
|
4.3
|
15.6
|
1.0
|
|
C2A
|
B:HEM804
|
4.3
|
15.7
|
1.0
|
|
C2D
|
B:HEM804
|
4.3
|
12.9
|
1.0
|
|
C3C
|
B:HEM804
|
4.3
|
16.1
|
1.0
|
|
C3A
|
B:HEM804
|
4.3
|
15.3
|
1.0
|
|
C2B
|
B:HEM804
|
4.3
|
15.4
|
1.0
|
|
C2C
|
B:HEM804
|
4.3
|
15.4
|
1.0
|
|
Reference:
C.G.Mowat,
K.L.Pankhurst,
C.S.Miles,
D.Leys,
M.D.Walkinshaw,
G.A.Reid,
S.K.Chapman.
Engineering Water to Act As the Active Site Acid Catalyst in A Soluble Fumarate Reductase Biochemistry V. 41 11990 2002.
ISSN: ISSN 0006-2960
PubMed: 12356299
DOI: 10.1021/BI0203177
Page generated: Sat Aug 3 09:48:34 2024
|
