Iron in PDB 1lm1: Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme

All present enzymatic activity of Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme:
1.4.7.1;

The structure of Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme, PDB code: 1lm1 was solved by R.H.Van Den Heuvel, D.Ferrari, R.T.Bossi, S.Ravasio, B.Curti, M.A.Vanoni, F.J.Florencio, A.Mattevi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	129.10 / 2.80
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	166.083, 166.083, 219.584, 90.00, 90.00, 90.00
R / Rfree (%)	23.6 / 28.7

The binding sites of Iron atom in the Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme (pdb code 1lm1). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme, PDB code: 1lm1:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2072 b:65.1 occ:1.00 FE1 A:F3S2072 0.0 65.1 1.0 SG A:CYS1137 1.9 34.8 1.0 S1 A:F3S2072 2.1 68.5 1.0 S2 A:F3S2072 2.2 61.8 1.0 S3 A:F3S2072 2.2 66.3 1.0 FE3 A:F3S2072 2.4 70.0 1.0 FE4 A:F3S2072 2.4 60.3 1.0 CB A:CYS1137 3.5 32.9 1.0 S4 A:F3S2072 3.8 63.9 1.0 SG A:CYS1148 3.9 31.5 1.0 CA A:CYS1137 4.0 33.6 1.0 O A:ILE1138 4.1 37.1 1.0 N A:ALA1140 4.2 31.2 1.0 SG A:CYS1143 4.2 31.9 1.0 CA A:MET1139 4.4 32.3 1.0 N A:ILE1138 4.5 33.0 1.0 C A:ILE1138 4.6 34.6 1.0 C A:MET1139 4.6 32.2 1.0 C A:CYS1137 4.7 32.6 1.0 N A:ARG1141 4.8 30.2 1.0 N A:MET1139 4.9 33.7 1.0 CB A:ALA1140 5.0 28.3 1.0

Iron binding site 2 out of 3 in the Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2072 b:70.0 occ:1.00 FE3 A:F3S2072 0.0 70.0 1.0 SG A:CYS1148 1.8 31.5 1.0 S3 A:F3S2072 2.2 66.3 1.0 S4 A:F3S2072 2.2 63.9 1.0 S1 A:F3S2072 2.2 68.5 1.0 FE1 A:F3S2072 2.4 65.1 1.0 FE4 A:F3S2072 2.4 60.3 1.0 CB A:CYS1148 3.3 28.5 1.0 S2 A:F3S2072 3.5 61.8 1.0 O A:HOH2113 3.7 69.1 1.0 CA A:CYS1148 3.9 28.3 1.0 SG A:CYS1137 4.0 34.8 1.0 CB A:ALA1140 4.1 28.3 1.0 CD A:PRO1149 4.4 25.6 1.0 SG A:CYS1143 4.4 31.9 1.0 N A:ALA1140 4.6 31.2 1.0 CB A:ALA1153 4.7 27.7 1.0 C A:CYS1148 4.9 27.3 1.0 N A:ARG1141 4.9 30.2 1.0 CA A:ALA1140 4.9 30.0 1.0 N A:ALA1153 4.9 29.7 1.0 N A:PRO1149 5.0 26.5 1.0 CB A:VAL1152 5.0 29.1 1.0 N A:CYS1148 5.0 29.3 1.0

Iron binding site 3 out of 3 in the Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase: Native Enzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe2072 b:60.3 occ:1.00 FE4 A:F3S2072 0.0 60.3 1.0 SG A:CYS1143 2.1 31.9 1.0 S2 A:F3S2072 2.2 61.8 1.0 S4 A:F3S2072 2.2 63.9 1.0 S3 A:F3S2072 2.2 66.3 1.0 FE3 A:F3S2072 2.4 70.0 1.0 FE1 A:F3S2072 2.4 65.1 1.0 CB A:CYS1143 3.5 31.5 1.0 S1 A:F3S2072 3.8 68.5 1.0 N A:CYS1143 3.8 30.2 1.0 CA A:CYS1143 3.9 30.9 1.0 SG A:CYS1148 3.9 31.5 1.0 SG A:CYS1137 4.1 34.8 1.0 N A:VAL1142 4.1 31.6 1.0 CB A:ALA1153 4.2 27.7 1.0 N A:ARG1141 4.3 30.2 1.0 CA A:ARG1141 4.5 30.4 1.0 C A:VAL1142 4.6 30.8 1.0 C A:ARG1141 4.9 31.6 1.0 CB A:CYS1148 5.0 28.5 1.0

R.H.Van Den Heuvel, D.Ferrari, R.T.Bossi, S.Ravasio, B.Curti, M.A.Vanoni, F.J.Florencio, A.Mattevi. Structural Studies on the Synchronization of Catalytic Centers in Glutamate Synthase J.Biol.Chem. V. 277 24579 2002.
ISSN: ISSN 0021-9258
PubMed: 11967268
DOI: 10.1074/JBC.M202541200