Iron in PDB 1m7s: Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae

All present enzymatic activity of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae:
1.11.1.6;

The structure of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae, PDB code: 1m7s was solved by X.Carpena, M.Soriano, M.G.Klotz, H.W.Duckworth, L.J.Donald, W.Melik-Adamyan, I.Fita, P.C.Loewen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	21.90 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	60.630, 153.870, 109.190, 90.00, 102.80, 90.00
R / Rfree (%)	18.7 / 24

The binding sites of Iron atom in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae (pdb code 1m7s). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae, PDB code: 1m7s:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe600 b:8.0 occ:1.00 FE A:HEM600 0.0 8.0 1.0 ND A:HEM600 2.0 4.9 1.0 OH A:TYR358 2.0 7.4 1.0 NC A:HEM600 2.0 8.4 1.0 NB A:HEM600 2.0 8.8 1.0 NA A:HEM600 2.0 7.8 1.0 O A:HOH1673 2.5 25.1 1.0 CZ A:TYR358 3.0 5.4 1.0 C1D A:HEM600 3.0 5.8 1.0 C1A A:HEM600 3.0 5.6 1.0 C1C A:HEM600 3.0 7.9 1.0 C4C A:HEM600 3.0 7.1 1.0 C4D A:HEM600 3.0 7.2 1.0 C4A A:HEM600 3.0 8.0 1.0 C1B A:HEM600 3.0 10.7 1.0 C4B A:HEM600 3.1 11.7 1.0 CHB A:HEM600 3.4 8.7 1.0 CHC A:HEM600 3.4 7.8 1.0 CHA A:HEM600 3.4 6.5 1.0 CHD A:HEM600 3.4 5.0 1.0 CE1 A:TYR358 3.7 4.5 1.0 CE2 A:TYR358 3.8 7.1 1.0 NE A:ARG354 4.1 9.1 1.0 NH2 A:ARG354 4.2 9.0 1.0 C2C A:HEM600 4.2 6.7 1.0 C3C A:HEM600 4.2 7.0 1.0 C2D A:HEM600 4.3 7.6 1.0 C3D A:HEM600 4.3 7.3 1.0 C3B A:HEM600 4.3 11.5 1.0 C2B A:HEM600 4.3 12.3 1.0 C3A A:HEM600 4.3 7.1 1.0 C2A A:HEM600 4.3 6.5 1.0 CZ A:PHE163 4.5 8.9 1.0 CZ A:ARG354 4.6 10.4 1.0 NE2 A:HIS78 4.6 7.7 1.0 CD2 A:HIS78 4.6 6.8 1.0 CG2 A:VAL77 4.6 5.5 1.0 O A:HOH1874 4.8 22.5 1.0 CD1 A:TYR358 4.9 4.5 1.0 CD2 A:TYR358 5.0 6.8 1.0

Iron binding site 2 out of 4 in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe600 b:14.4 occ:1.00 FE B:HEM600 0.0 14.4 1.0 OH B:TYR358 1.9 11.9 1.0 NA B:HEM600 2.0 15.2 1.0 NC B:HEM600 2.0 14.4 1.0 NB B:HEM600 2.0 14.6 1.0 ND B:HEM600 2.0 12.1 1.0 O B:HOH3125 2.2 37.6 1.0 CZ B:TYR358 3.0 12.1 1.0 C1B B:HEM600 3.0 15.4 1.0 C1A B:HEM600 3.0 15.1 1.0 C4A B:HEM600 3.0 15.1 1.0 C4B B:HEM600 3.0 16.7 1.0 C1D B:HEM600 3.0 14.4 1.0 C4C B:HEM600 3.0 13.5 1.0 C1C B:HEM600 3.0 15.7 1.0 C4D B:HEM600 3.1 12.1 1.0 CHB B:HEM600 3.4 14.3 1.0 CHD B:HEM600 3.4 11.4 1.0 CHA B:HEM600 3.4 12.4 1.0 CHC B:HEM600 3.4 16.0 1.0 CE1 B:TYR358 3.7 13.1 1.0 CE2 B:TYR358 3.8 12.8 1.0 O B:HOH1419 4.1 36.9 1.0 NE B:ARG354 4.2 13.6 1.0 C2A B:HEM600 4.2 14.0 1.0 C2B B:HEM600 4.2 16.8 1.0 C3A B:HEM600 4.2 14.4 1.0 C3B B:HEM600 4.2 18.1 1.0 NH2 B:ARG354 4.3 16.7 1.0 C2C B:HEM600 4.3 15.2 1.0 C3C B:HEM600 4.3 14.8 1.0 C3D B:HEM600 4.3 11.3 1.0 C2D B:HEM600 4.3 12.0 1.0 CZ B:PHE163 4.6 15.8 1.0 CG2 B:VAL77 4.6 13.4 1.0 CZ B:ARG354 4.6 15.9 1.0 NE2 B:HIS78 4.7 16.2 1.0 CD2 B:HIS78 4.7 14.2 1.0 CD1 B:TYR358 4.9 13.0 1.0

Iron binding site 3 out of 4 in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe600 b:13.9 occ:1.00 FE C:HEM600 0.0 13.9 1.0 OH C:TYR358 1.9 13.7 1.0 NA C:HEM600 2.0 14.3 1.0 O C:HOH1061 2.0 24.6 1.0 NC C:HEM600 2.0 13.0 1.0 NB C:HEM600 2.0 15.6 1.0 ND C:HEM600 2.0 10.5 1.0 CZ C:TYR358 3.0 11.6 1.0 C1C C:HEM600 3.0 14.4 1.0 C4A C:HEM600 3.0 13.8 1.0 C1B C:HEM600 3.0 17.2 1.0 C4C C:HEM600 3.0 12.7 1.0 C1A C:HEM600 3.0 15.1 1.0 C1D C:HEM600 3.0 11.4 1.0 C4B C:HEM600 3.0 17.2 1.0 C4D C:HEM600 3.1 12.2 1.0 CHB C:HEM600 3.4 14.7 1.0 CHA C:HEM600 3.4 15.0 1.0 CHC C:HEM600 3.4 16.1 1.0 CHD C:HEM600 3.4 12.3 1.0 CE1 C:TYR358 3.7 13.6 1.0 CE2 C:TYR358 3.8 13.3 1.0 NE C:ARG354 4.1 13.2 1.0 O C:HOH1095 4.2 29.6 1.0 C2C C:HEM600 4.2 14.1 1.0 C3C C:HEM600 4.2 14.0 1.0 C3A C:HEM600 4.2 13.6 1.0 C2A C:HEM600 4.2 15.4 1.0 NH2 C:ARG354 4.3 12.2 1.0 C2B C:HEM600 4.3 18.6 1.0 C3B C:HEM600 4.3 19.3 1.0 C3D C:HEM600 4.3 11.2 1.0 C2D C:HEM600 4.3 10.4 1.0 CZ C:PHE163 4.5 16.2 1.0 CZ C:ARG354 4.7 14.8 1.0 CG2 C:VAL77 4.7 13.5 1.0 NE2 C:HIS78 4.7 17.4 1.0 CD2 C:HIS78 4.7 16.5 1.0 CD1 C:TYR358 4.9 12.6 1.0

Iron binding site 4 out of 4 in the Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure Analysis of Catalase Catf of Pseudomonas Syringae within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe600 b:8.1 occ:1.00 FE D:HEM600 0.0 8.1 1.0 OH D:TYR358 2.0 8.9 1.0 NA D:HEM600 2.0 8.5 1.0 ND D:HEM600 2.0 7.4 1.0 NB D:HEM600 2.0 9.1 1.0 NC D:HEM600 2.0 8.2 1.0 O D:HOH1242 2.6 28.0 1.0 CZ D:TYR358 3.0 6.3 1.0 C1B D:HEM600 3.0 11.1 1.0 C4A D:HEM600 3.0 8.8 1.0 C4C D:HEM600 3.0 7.1 1.0 C1D D:HEM600 3.0 7.2 1.0 C1A D:HEM600 3.0 7.3 1.0 C1C D:HEM600 3.0 8.9 1.0 C4B D:HEM600 3.1 11.4 1.0 C4D D:HEM600 3.1 7.8 1.0 CHB D:HEM600 3.4 8.5 1.0 CHD D:HEM600 3.4 5.5 1.0 CHA D:HEM600 3.5 7.0 1.0 CHC D:HEM600 3.5 9.3 1.0 CE1 D:TYR358 3.7 7.0 1.0 CE2 D:TYR358 3.8 4.0 1.0 NE D:ARG354 4.1 5.1 1.0 C2A D:HEM600 4.2 7.5 1.0 C3C D:HEM600 4.3 7.8 1.0 C3A D:HEM600 4.3 6.6 1.0 C2C D:HEM600 4.3 7.0 1.0 C2B D:HEM600 4.3 12.5 1.0 C3B D:HEM600 4.3 12.6 1.0 C2D D:HEM600 4.3 6.3 1.0 O D:HOH1096 4.3 23.3 1.0 C3D D:HEM600 4.3 5.1 1.0 NH2 D:ARG354 4.3 7.5 1.0 CZ D:PHE163 4.5 8.5 1.0 CG2 D:VAL77 4.7 5.3 1.0 CZ D:ARG354 4.7 6.6 1.0 NE2 D:HIS78 4.7 9.6 1.0 CD2 D:HIS78 4.7 7.9 1.0 CD1 D:TYR358 5.0 7.4 1.0 CE1 D:PHE163 5.0 10.7 1.0 CD2 D:TYR358 5.0 6.3 1.0

X.Carpena, M.Soriano, M.G.Klotz, H.W.Duckworth, L.J.Donald, W.Melik-Adamyan, I.Fita, P.C.Loewen. Structure of the Clade 1 Catalase, Catf of Pseudomonas Syringae, at 1.8 A Resolution Proteins V. 50 423 2003.
ISSN: ISSN 0887-3585
PubMed: 12557185
DOI: 10.1002/PROT.10284