Iron in PDB 1n90: Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
Enzymatic activity of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
All present enzymatic activity of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa:
1.9.3.2;
Protein crystallography data
The structure of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa, PDB code: 1n90
was solved by
D.Nurizzo,
M.Tegoni,
C.Cambillau,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
12.00 /
2.90
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
164.370,
89.530,
112.110,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
20.4 /
24.1
|
Iron Binding Sites:
The binding sites of Iron atom in the Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
(pdb code 1n90). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the
Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa, PDB code: 1n90:
Jump to Iron binding site number:
1;
2;
3;
4;
Iron binding site 1 out
of 4 in 1n90
Go back to
Iron Binding Sites List in 1n90
Iron binding site 1 out
of 4 in the Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe601
b:24.8
occ:1.00
|
FE
|
A:HEC601
|
0.0
|
24.8
|
1.0
|
NA
|
A:HEC601
|
1.9
|
24.8
|
1.0
|
ND
|
A:HEC601
|
2.0
|
24.8
|
1.0
|
NE2
|
A:HIS51
|
2.0
|
24.0
|
1.0
|
NB
|
A:HEC601
|
2.0
|
24.8
|
1.0
|
NC
|
A:HEC601
|
2.0
|
24.8
|
1.0
|
SD
|
A:MET88
|
2.3
|
33.8
|
1.0
|
CE1
|
A:HIS51
|
2.8
|
24.0
|
1.0
|
C4A
|
A:HEC601
|
2.9
|
24.8
|
1.0
|
C1A
|
A:HEC601
|
3.0
|
24.8
|
1.0
|
C4D
|
A:HEC601
|
3.0
|
24.8
|
1.0
|
C1B
|
A:HEC601
|
3.0
|
24.8
|
1.0
|
C1D
|
A:HEC601
|
3.1
|
24.8
|
1.0
|
C4C
|
A:HEC601
|
3.1
|
24.8
|
1.0
|
C4B
|
A:HEC601
|
3.1
|
24.8
|
1.0
|
C1C
|
A:HEC601
|
3.1
|
24.8
|
1.0
|
CD2
|
A:HIS51
|
3.2
|
24.0
|
1.0
|
CHB
|
A:HEC601
|
3.3
|
24.8
|
1.0
|
CHA
|
A:HEC601
|
3.4
|
24.8
|
1.0
|
CHD
|
A:HEC601
|
3.4
|
24.8
|
1.0
|
CHC
|
A:HEC601
|
3.5
|
24.8
|
1.0
|
CE
|
A:MET88
|
3.5
|
33.8
|
1.0
|
CG
|
A:MET88
|
3.7
|
33.8
|
1.0
|
ND1
|
A:HIS51
|
4.0
|
24.0
|
1.0
|
C3A
|
A:HEC601
|
4.2
|
24.8
|
1.0
|
C2A
|
A:HEC601
|
4.2
|
24.8
|
1.0
|
CG
|
A:HIS51
|
4.2
|
24.0
|
1.0
|
C2B
|
A:HEC601
|
4.2
|
24.8
|
1.0
|
CB
|
A:MET88
|
4.2
|
33.8
|
1.0
|
C3D
|
A:HEC601
|
4.2
|
24.8
|
1.0
|
C3B
|
A:HEC601
|
4.3
|
24.8
|
1.0
|
C3C
|
A:HEC601
|
4.3
|
24.8
|
1.0
|
C2D
|
A:HEC601
|
4.3
|
24.8
|
1.0
|
C2C
|
A:HEC601
|
4.3
|
24.8
|
1.0
|
CA
|
A:MET88
|
4.7
|
31.6
|
1.0
|
|
Iron binding site 2 out
of 4 in 1n90
Go back to
Iron Binding Sites List in 1n90
Iron binding site 2 out
of 4 in the Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:28.8
occ:1.00
|
FE
|
A:DHE602
|
0.0
|
28.8
|
1.0
|
NE2
|
A:HIS182
|
2.0
|
26.5
|
1.0
|
ND
|
A:DHE602
|
2.0
|
28.8
|
1.0
|
NA
|
A:DHE602
|
2.1
|
28.8
|
1.0
|
NC
|
A:DHE602
|
2.1
|
28.8
|
1.0
|
NB
|
A:DHE602
|
2.1
|
28.8
|
1.0
|
CE1
|
A:HIS182
|
3.0
|
26.5
|
1.0
|
CD2
|
A:HIS182
|
3.0
|
26.5
|
1.0
|
C1C
|
A:DHE602
|
3.0
|
28.8
|
1.0
|
C1D
|
A:DHE602
|
3.0
|
28.8
|
1.0
|
C4D
|
A:DHE602
|
3.0
|
28.8
|
1.0
|
C1A
|
A:DHE602
|
3.1
|
28.8
|
1.0
|
C1B
|
A:DHE602
|
3.1
|
28.8
|
1.0
|
C4A
|
A:DHE602
|
3.1
|
28.8
|
1.0
|
C4B
|
A:DHE602
|
3.1
|
28.8
|
1.0
|
C4C
|
A:DHE602
|
3.1
|
28.8
|
1.0
|
CHA
|
A:DHE602
|
3.4
|
28.8
|
1.0
|
CHC
|
A:DHE602
|
3.4
|
28.8
|
1.0
|
CHD
|
A:DHE602
|
3.4
|
28.8
|
1.0
|
CHB
|
A:DHE602
|
3.4
|
28.8
|
1.0
|
ND1
|
A:HIS182
|
4.1
|
26.5
|
1.0
|
CG
|
A:HIS182
|
4.1
|
26.5
|
1.0
|
OH
|
B:TYR10
|
4.2
|
52.3
|
1.0
|
C3D
|
A:DHE602
|
4.2
|
28.8
|
1.0
|
C2D
|
A:DHE602
|
4.2
|
28.8
|
1.0
|
C2A
|
A:DHE602
|
4.3
|
28.8
|
1.0
|
C3A
|
A:DHE602
|
4.3
|
28.8
|
1.0
|
C2C
|
A:DHE602
|
4.3
|
28.8
|
1.0
|
C2B
|
A:DHE602
|
4.3
|
28.8
|
1.0
|
C3B
|
A:DHE602
|
4.4
|
28.8
|
1.0
|
C3C
|
A:DHE602
|
4.4
|
28.8
|
1.0
|
CE1
|
B:TYR10
|
5.0
|
52.3
|
1.0
|
CE1
|
A:HIS327
|
5.0
|
25.5
|
1.0
|
|
Iron binding site 3 out
of 4 in 1n90
Go back to
Iron Binding Sites List in 1n90
Iron binding site 3 out
of 4 in the Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:31.3
occ:1.00
|
FE
|
B:HEC601
|
0.0
|
31.3
|
1.0
|
NA
|
B:HEC601
|
1.9
|
31.3
|
1.0
|
ND
|
B:HEC601
|
1.9
|
31.3
|
1.0
|
NE2
|
B:HIS51
|
2.0
|
32.6
|
1.0
|
NB
|
B:HEC601
|
2.0
|
31.3
|
1.0
|
NC
|
B:HEC601
|
2.0
|
31.3
|
1.0
|
SD
|
B:MET88
|
2.4
|
26.3
|
1.0
|
C4A
|
B:HEC601
|
2.9
|
31.3
|
1.0
|
C4D
|
B:HEC601
|
3.0
|
31.3
|
1.0
|
C1A
|
B:HEC601
|
3.0
|
31.3
|
1.0
|
CE1
|
B:HIS51
|
3.0
|
32.6
|
1.0
|
C1D
|
B:HEC601
|
3.0
|
31.3
|
1.0
|
C4B
|
B:HEC601
|
3.0
|
31.3
|
1.0
|
CD2
|
B:HIS51
|
3.1
|
32.6
|
1.0
|
C1B
|
B:HEC601
|
3.1
|
31.3
|
1.0
|
C4C
|
B:HEC601
|
3.1
|
31.3
|
1.0
|
C1C
|
B:HEC601
|
3.1
|
31.3
|
1.0
|
CHA
|
B:HEC601
|
3.3
|
31.3
|
1.0
|
CHB
|
B:HEC601
|
3.3
|
31.3
|
1.0
|
CHD
|
B:HEC601
|
3.4
|
31.3
|
1.0
|
CHC
|
B:HEC601
|
3.5
|
31.3
|
1.0
|
CE
|
B:MET88
|
3.6
|
26.3
|
1.0
|
CG
|
B:MET88
|
3.7
|
26.3
|
1.0
|
ND1
|
B:HIS51
|
4.1
|
32.6
|
1.0
|
C3A
|
B:HEC601
|
4.1
|
31.3
|
1.0
|
C2A
|
B:HEC601
|
4.2
|
31.3
|
1.0
|
CG
|
B:HIS51
|
4.2
|
32.6
|
1.0
|
CB
|
B:MET88
|
4.2
|
26.3
|
1.0
|
C3D
|
B:HEC601
|
4.2
|
31.3
|
1.0
|
C3B
|
B:HEC601
|
4.2
|
31.3
|
1.0
|
C2D
|
B:HEC601
|
4.2
|
31.3
|
1.0
|
C2B
|
B:HEC601
|
4.3
|
31.3
|
1.0
|
C2C
|
B:HEC601
|
4.3
|
31.3
|
1.0
|
C3C
|
B:HEC601
|
4.3
|
31.3
|
1.0
|
CA
|
B:MET88
|
4.7
|
31.9
|
1.0
|
|
Iron binding site 4 out
of 4 in 1n90
Go back to
Iron Binding Sites List in 1n90
Iron binding site 4 out
of 4 in the Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Following the C Heme Reduction in Nitrite Reductase From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe602
b:24.9
occ:1.00
|
FE
|
B:DHE602
|
0.0
|
24.9
|
1.0
|
NE2
|
B:HIS182
|
2.0
|
29.2
|
1.0
|
ND
|
B:DHE602
|
2.0
|
24.9
|
1.0
|
NC
|
B:DHE602
|
2.0
|
24.9
|
1.0
|
NA
|
B:DHE602
|
2.1
|
24.9
|
1.0
|
NB
|
B:DHE602
|
2.1
|
24.9
|
1.0
|
CD2
|
B:HIS182
|
3.0
|
29.2
|
1.0
|
CE1
|
B:HIS182
|
3.0
|
29.2
|
1.0
|
C1C
|
B:DHE602
|
3.0
|
24.9
|
1.0
|
C1D
|
B:DHE602
|
3.0
|
24.9
|
1.0
|
C4C
|
B:DHE602
|
3.0
|
24.9
|
1.0
|
C4D
|
B:DHE602
|
3.0
|
24.9
|
1.0
|
C1A
|
B:DHE602
|
3.1
|
24.9
|
1.0
|
C1B
|
B:DHE602
|
3.1
|
24.9
|
1.0
|
C4A
|
B:DHE602
|
3.1
|
24.9
|
1.0
|
C4B
|
B:DHE602
|
3.1
|
24.9
|
1.0
|
CHD
|
B:DHE602
|
3.3
|
24.9
|
1.0
|
CHA
|
B:DHE602
|
3.4
|
24.9
|
1.0
|
CHC
|
B:DHE602
|
3.4
|
24.9
|
1.0
|
CHB
|
B:DHE602
|
3.4
|
24.9
|
1.0
|
OH
|
A:TYR10
|
4.1
|
54.0
|
1.0
|
ND1
|
B:HIS182
|
4.1
|
29.2
|
1.0
|
CG
|
B:HIS182
|
4.1
|
29.2
|
1.0
|
C2D
|
B:DHE602
|
4.2
|
24.9
|
1.0
|
C3D
|
B:DHE602
|
4.2
|
24.9
|
1.0
|
C2A
|
B:DHE602
|
4.3
|
24.9
|
1.0
|
C3A
|
B:DHE602
|
4.3
|
24.9
|
1.0
|
C2C
|
B:DHE602
|
4.3
|
24.9
|
1.0
|
C2B
|
B:DHE602
|
4.3
|
24.9
|
1.0
|
C3C
|
B:DHE602
|
4.4
|
24.9
|
1.0
|
C3B
|
B:DHE602
|
4.4
|
24.9
|
1.0
|
CE1
|
A:TYR10
|
4.9
|
54.0
|
1.0
|
CZ
|
A:TYR10
|
4.9
|
54.0
|
1.0
|
|
Reference:
D.Nurizzo,
F.Cutruzzola,
M.Arese,
D.Bourgeois,
M.Brunori,
C.Cambillau,
M.Tegoni.
Does the Reduction of C Heme Trigger the Conformational Change of Crystalline Nitrite Reductase? J.Biol.Chem. V. 274 14997 1999.
ISSN: ISSN 0021-9258
PubMed: 10329702
DOI: 10.1074/JBC.274.21.14997
Page generated: Sat Aug 3 11:26:18 2024
|