Iron in PDB 1p2h: H61M Mutant of Flavocytochrome C3
Enzymatic activity of H61M Mutant of Flavocytochrome C3
All present enzymatic activity of H61M Mutant of Flavocytochrome C3:
1.3.99.1;
Protein crystallography data
The structure of H61M Mutant of Flavocytochrome C3, PDB code: 1p2h
was solved by
E.L.Rothery,
C.G.Mowat,
C.S.Miles,
M.D.Walkinshaw,
G.A.Reid,
S.K.Chapman,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
17.00 /
2.10
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
44.693,
87.286,
76.993,
90.00,
104.35,
90.00
|
R / Rfree (%)
|
19.5 /
26.1
|
Other elements in 1p2h:
The structure of H61M Mutant of Flavocytochrome C3 also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the H61M Mutant of Flavocytochrome C3
(pdb code 1p2h). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the
H61M Mutant of Flavocytochrome C3, PDB code: 1p2h:
Jump to Iron binding site number:
1;
2;
3;
4;
Iron binding site 1 out
of 4 in 1p2h
Go back to
Iron Binding Sites List in 1p2h
Iron binding site 1 out
of 4 in the H61M Mutant of Flavocytochrome C3
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of H61M Mutant of Flavocytochrome C3 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe801
b:24.2
occ:1.00
|
FE
|
A:HEM801
|
0.0
|
24.2
|
1.0
|
NA
|
A:HEM801
|
2.0
|
24.4
|
1.0
|
NC
|
A:HEM801
|
2.0
|
29.1
|
1.0
|
NE2
|
A:HIS18
|
2.0
|
23.8
|
1.0
|
NE2
|
A:HIS75
|
2.0
|
26.8
|
1.0
|
NB
|
A:HEM801
|
2.0
|
30.1
|
1.0
|
ND
|
A:HEM801
|
2.0
|
29.1
|
1.0
|
CE1
|
A:HIS75
|
2.8
|
26.8
|
1.0
|
CE1
|
A:HIS18
|
2.8
|
27.7
|
1.0
|
C1C
|
A:HEM801
|
3.0
|
31.2
|
1.0
|
C4A
|
A:HEM801
|
3.0
|
27.1
|
1.0
|
C4C
|
A:HEM801
|
3.0
|
32.1
|
1.0
|
C4B
|
A:HEM801
|
3.0
|
29.2
|
1.0
|
C1A
|
A:HEM801
|
3.0
|
25.4
|
1.0
|
C1B
|
A:HEM801
|
3.1
|
29.2
|
1.0
|
C1D
|
A:HEM801
|
3.1
|
27.7
|
1.0
|
C4D
|
A:HEM801
|
3.1
|
25.1
|
1.0
|
CD2
|
A:HIS18
|
3.2
|
27.2
|
1.0
|
CD2
|
A:HIS75
|
3.2
|
27.9
|
1.0
|
CHC
|
A:HEM801
|
3.3
|
30.4
|
1.0
|
CHD
|
A:HEM801
|
3.4
|
29.6
|
1.0
|
CHA
|
A:HEM801
|
3.4
|
20.7
|
1.0
|
CHB
|
A:HEM801
|
3.4
|
28.0
|
1.0
|
ND1
|
A:HIS75
|
4.0
|
27.0
|
1.0
|
ND1
|
A:HIS18
|
4.0
|
27.6
|
1.0
|
C2C
|
A:HEM801
|
4.2
|
33.6
|
1.0
|
C3C
|
A:HEM801
|
4.2
|
34.4
|
1.0
|
C2A
|
A:HEM801
|
4.2
|
25.4
|
1.0
|
C3A
|
A:HEM801
|
4.2
|
25.8
|
1.0
|
C3B
|
A:HEM801
|
4.2
|
32.0
|
1.0
|
CG
|
A:HIS18
|
4.2
|
27.3
|
1.0
|
C2B
|
A:HEM801
|
4.2
|
31.6
|
1.0
|
CG
|
A:HIS75
|
4.3
|
28.0
|
1.0
|
C2D
|
A:HEM801
|
4.3
|
27.6
|
1.0
|
C3D
|
A:HEM801
|
4.3
|
24.7
|
1.0
|
|
Iron binding site 2 out
of 4 in 1p2h
Go back to
Iron Binding Sites List in 1p2h
Iron binding site 2 out
of 4 in the H61M Mutant of Flavocytochrome C3
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of H61M Mutant of Flavocytochrome C3 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe802
b:44.8
occ:1.00
|
FE
|
A:HEM802
|
0.0
|
44.8
|
1.0
|
NA
|
A:HEM802
|
2.0
|
48.3
|
1.0
|
NC
|
A:HEM802
|
2.0
|
49.9
|
1.0
|
ND
|
A:HEM802
|
2.0
|
51.4
|
1.0
|
NE2
|
A:HIS8
|
2.0
|
33.0
|
1.0
|
NB
|
A:HEM802
|
2.0
|
48.4
|
1.0
|
NE2
|
A:HIS40
|
2.2
|
36.6
|
1.0
|
CE1
|
A:HIS40
|
2.8
|
37.5
|
1.0
|
CD2
|
A:HIS8
|
3.0
|
32.8
|
1.0
|
C1C
|
A:HEM802
|
3.0
|
50.8
|
1.0
|
C4B
|
A:HEM802
|
3.0
|
47.4
|
1.0
|
C1A
|
A:HEM802
|
3.0
|
50.5
|
1.0
|
C4A
|
A:HEM802
|
3.0
|
48.6
|
1.0
|
C4C
|
A:HEM802
|
3.0
|
51.5
|
1.0
|
C1B
|
A:HEM802
|
3.0
|
47.0
|
1.0
|
C1D
|
A:HEM802
|
3.0
|
51.5
|
1.0
|
C4D
|
A:HEM802
|
3.0
|
52.1
|
1.0
|
CE1
|
A:HIS8
|
3.0
|
33.6
|
1.0
|
CD2
|
A:HIS40
|
3.3
|
36.9
|
1.0
|
CHC
|
A:HEM802
|
3.4
|
49.8
|
1.0
|
CHA
|
A:HEM802
|
3.4
|
52.0
|
1.0
|
CHD
|
A:HEM802
|
3.4
|
50.9
|
1.0
|
CHB
|
A:HEM802
|
3.4
|
48.2
|
1.0
|
ND1
|
A:HIS40
|
4.0
|
37.8
|
1.0
|
CG
|
A:HIS8
|
4.1
|
34.1
|
1.0
|
C3B
|
A:HEM802
|
4.2
|
47.0
|
1.0
|
ND1
|
A:HIS8
|
4.2
|
32.4
|
1.0
|
C2B
|
A:HEM802
|
4.2
|
46.8
|
1.0
|
C3A
|
A:HEM802
|
4.2
|
49.6
|
1.0
|
C2A
|
A:HEM802
|
4.2
|
50.2
|
1.0
|
C2C
|
A:HEM802
|
4.2
|
52.5
|
1.0
|
C3C
|
A:HEM802
|
4.2
|
53.1
|
1.0
|
C3D
|
A:HEM802
|
4.3
|
53.9
|
1.0
|
C2D
|
A:HEM802
|
4.3
|
53.7
|
1.0
|
CG
|
A:HIS40
|
4.3
|
37.4
|
1.0
|
|
Iron binding site 3 out
of 4 in 1p2h
Go back to
Iron Binding Sites List in 1p2h
Iron binding site 3 out
of 4 in the H61M Mutant of Flavocytochrome C3
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of H61M Mutant of Flavocytochrome C3 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe803
b:34.1
occ:1.00
|
FE
|
A:HEM803
|
0.0
|
34.1
|
1.0
|
ND
|
A:HEM803
|
2.0
|
35.3
|
1.0
|
NB
|
A:HEM803
|
2.0
|
36.5
|
1.0
|
NA
|
A:HEM803
|
2.0
|
39.5
|
1.0
|
NC
|
A:HEM803
|
2.1
|
34.2
|
1.0
|
NE2
|
A:HIS72
|
2.2
|
31.9
|
1.0
|
NE2
|
A:HIS58
|
2.2
|
36.8
|
1.0
|
C1A
|
A:HEM803
|
3.0
|
39.0
|
1.0
|
C1B
|
A:HEM803
|
3.0
|
38.7
|
1.0
|
C4D
|
A:HEM803
|
3.0
|
38.6
|
1.0
|
C4A
|
A:HEM803
|
3.0
|
39.4
|
1.0
|
C1D
|
A:HEM803
|
3.0
|
36.4
|
1.0
|
CD2
|
A:HIS72
|
3.0
|
29.5
|
1.0
|
C4B
|
A:HEM803
|
3.0
|
37.9
|
1.0
|
C4C
|
A:HEM803
|
3.1
|
30.8
|
1.0
|
CE1
|
A:HIS58
|
3.1
|
35.0
|
1.0
|
C1C
|
A:HEM803
|
3.1
|
29.5
|
1.0
|
CE1
|
A:HIS72
|
3.2
|
33.0
|
1.0
|
CD2
|
A:HIS58
|
3.3
|
38.2
|
1.0
|
CHA
|
A:HEM803
|
3.3
|
39.2
|
1.0
|
CHB
|
A:HEM803
|
3.3
|
38.9
|
1.0
|
CHD
|
A:HEM803
|
3.4
|
32.8
|
1.0
|
CHC
|
A:HEM803
|
3.5
|
33.3
|
1.0
|
C2B
|
A:HEM803
|
4.2
|
38.6
|
1.0
|
CG
|
A:HIS72
|
4.2
|
31.4
|
1.0
|
C2A
|
A:HEM803
|
4.2
|
40.9
|
1.0
|
C3A
|
A:HEM803
|
4.2
|
40.9
|
1.0
|
C3B
|
A:HEM803
|
4.2
|
38.0
|
1.0
|
C2D
|
A:HEM803
|
4.2
|
35.1
|
1.0
|
C3D
|
A:HEM803
|
4.2
|
35.7
|
1.0
|
ND1
|
A:HIS58
|
4.2
|
35.6
|
1.0
|
ND1
|
A:HIS72
|
4.3
|
29.1
|
1.0
|
C3C
|
A:HEM803
|
4.3
|
29.2
|
1.0
|
C2C
|
A:HEM803
|
4.3
|
30.6
|
1.0
|
CG
|
A:HIS58
|
4.4
|
36.1
|
1.0
|
SD
|
A:MET92
|
4.9
|
34.5
|
1.0
|
CG1
|
A:VAL46
|
5.0
|
36.9
|
1.0
|
|
Iron binding site 4 out
of 4 in 1p2h
Go back to
Iron Binding Sites List in 1p2h
Iron binding site 4 out
of 4 in the H61M Mutant of Flavocytochrome C3
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of H61M Mutant of Flavocytochrome C3 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe804
b:26.1
occ:1.00
|
FE
|
A:HEM804
|
0.0
|
26.1
|
1.0
|
NE2
|
A:HIS86
|
1.8
|
14.1
|
1.0
|
NC
|
A:HEM804
|
2.0
|
27.5
|
1.0
|
NA
|
A:HEM804
|
2.0
|
31.2
|
1.0
|
NB
|
A:HEM804
|
2.0
|
34.8
|
1.0
|
ND
|
A:HEM804
|
2.0
|
26.5
|
1.0
|
O
|
A:HOH1351
|
2.1
|
35.2
|
1.0
|
CE1
|
A:HIS86
|
2.7
|
20.0
|
1.0
|
CD2
|
A:HIS86
|
3.0
|
17.2
|
1.0
|
C1C
|
A:HEM804
|
3.0
|
30.0
|
1.0
|
C4C
|
A:HEM804
|
3.0
|
26.6
|
1.0
|
C4A
|
A:HEM804
|
3.0
|
32.9
|
1.0
|
C1B
|
A:HEM804
|
3.0
|
33.4
|
1.0
|
C4B
|
A:HEM804
|
3.0
|
34.6
|
1.0
|
C1D
|
A:HEM804
|
3.1
|
25.2
|
1.0
|
C1A
|
A:HEM804
|
3.1
|
30.5
|
1.0
|
C4D
|
A:HEM804
|
3.1
|
24.9
|
1.0
|
CHC
|
A:HEM804
|
3.4
|
33.1
|
1.0
|
CHD
|
A:HEM804
|
3.4
|
25.1
|
1.0
|
CHB
|
A:HEM804
|
3.4
|
31.6
|
1.0
|
CHA
|
A:HEM804
|
3.5
|
26.0
|
1.0
|
O
|
A:HOH869
|
3.9
|
31.6
|
1.0
|
ND1
|
A:HIS86
|
3.9
|
17.6
|
1.0
|
CG
|
A:HIS86
|
4.1
|
16.3
|
1.0
|
C3B
|
A:HEM804
|
4.2
|
37.2
|
1.0
|
C2B
|
A:HEM804
|
4.2
|
36.7
|
1.0
|
C2C
|
A:HEM804
|
4.2
|
30.9
|
1.0
|
C3C
|
A:HEM804
|
4.2
|
29.5
|
1.0
|
C3A
|
A:HEM804
|
4.2
|
32.5
|
1.0
|
C2A
|
A:HEM804
|
4.3
|
31.8
|
1.0
|
C2D
|
A:HEM804
|
4.3
|
24.8
|
1.0
|
C3D
|
A:HEM804
|
4.3
|
24.4
|
1.0
|
OG
|
A:SER60
|
4.6
|
54.3
|
1.0
|
|
Reference:
E.L.Rothery,
C.G.Mowat,
C.S.Miles,
M.D.Walkinshaw,
G.A.Reid,
S.K.Chapman.
Histidine 61: An Important Heme Ligand in the Soluble Fumarate Reductase From Shewanella Frigidimarina Biochemistry V. 42 13160 2003.
ISSN: ISSN 0006-2960
PubMed: 14609326
DOI: 10.1021/BI030159Z
Page generated: Sat Aug 3 12:56:04 2024
|