Iron in PDB 1q0c: Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
Enzymatic activity of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
All present enzymatic activity of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate):
1.13.11.15;
Protein crystallography data
The structure of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate), PDB code: 1q0c
was solved by
M.W.Vetting,
L.P.Wackett,
L.Que,
J.D.Lipscomb,
D.H.Ohlendorf,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
20.00 /
2.10
|
Space group
|
I 41
|
Cell size a, b, c (Å), α, β, γ (°)
|
157.000,
157.000,
122.300,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
17 /
23.4
|
Iron Binding Sites:
The binding sites of Iron atom in the Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
(pdb code 1q0c). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the
Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate), PDB code: 1q0c:
Jump to Iron binding site number:
1;
2;
3;
4;
Iron binding site 1 out
of 4 in 1q0c
Go back to
Iron Binding Sites List in 1q0c
Iron binding site 1 out
of 4 in the Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe500
b:43.8
occ:1.00
|
O3
|
A:DHY999
|
2.0
|
50.6
|
1.0
|
OE1
|
A:GLU267
|
2.1
|
35.0
|
1.0
|
NE2
|
A:HIS155
|
2.2
|
31.3
|
1.0
|
NE2
|
A:HIS214
|
2.3
|
33.5
|
1.0
|
O4
|
A:DHY999
|
2.3
|
45.7
|
1.0
|
C3
|
A:DHY999
|
3.1
|
46.5
|
1.0
|
CD
|
A:GLU267
|
3.1
|
34.3
|
1.0
|
CE1
|
A:HIS155
|
3.1
|
32.2
|
1.0
|
C4
|
A:DHY999
|
3.2
|
47.3
|
1.0
|
CE1
|
A:HIS214
|
3.2
|
33.1
|
1.0
|
CD2
|
A:HIS214
|
3.2
|
33.7
|
1.0
|
CD2
|
A:HIS155
|
3.3
|
30.0
|
1.0
|
OE2
|
A:GLU267
|
3.6
|
34.0
|
1.0
|
OH
|
A:TYR257
|
3.9
|
31.6
|
1.0
|
NE2
|
A:HIS200
|
4.0
|
33.5
|
1.0
|
ND1
|
A:HIS214
|
4.3
|
35.4
|
1.0
|
ND1
|
A:HIS155
|
4.3
|
29.2
|
1.0
|
CG
|
A:HIS214
|
4.3
|
36.1
|
1.0
|
CG
|
A:GLU267
|
4.3
|
30.8
|
1.0
|
CG
|
A:HIS155
|
4.4
|
31.4
|
1.0
|
C2
|
A:DHY999
|
4.4
|
46.8
|
1.0
|
CB
|
A:ALA216
|
4.4
|
28.5
|
1.0
|
CB
|
A:GLU267
|
4.4
|
28.8
|
1.0
|
ND2
|
A:ASN157
|
4.5
|
46.3
|
1.0
|
CE1
|
A:TYR257
|
4.5
|
28.5
|
1.0
|
C5
|
A:DHY999
|
4.6
|
46.4
|
1.0
|
CZ
|
A:TYR257
|
4.6
|
28.6
|
1.0
|
CE1
|
A:HIS200
|
4.7
|
35.7
|
1.0
|
CB
|
A:ASN157
|
4.7
|
41.9
|
1.0
|
CD2
|
A:TYR269
|
5.0
|
41.0
|
1.0
|
NE2
|
A:HIS248
|
5.0
|
41.8
|
1.0
|
|
Iron binding site 2 out
of 4 in 1q0c
Go back to
Iron Binding Sites List in 1q0c
Iron binding site 2 out
of 4 in the Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe500
b:42.5
occ:1.00
|
OE1
|
B:GLU267
|
1.9
|
36.7
|
1.0
|
O3
|
B:DHY999
|
2.0
|
41.5
|
1.0
|
NE2
|
B:HIS214
|
2.1
|
27.1
|
1.0
|
NE2
|
B:HIS155
|
2.3
|
32.0
|
1.0
|
O4
|
B:DHY999
|
2.4
|
43.6
|
1.0
|
CE1
|
B:HIS214
|
3.0
|
29.9
|
1.0
|
CD
|
B:GLU267
|
3.1
|
32.8
|
1.0
|
C3
|
B:DHY999
|
3.1
|
41.5
|
1.0
|
CE1
|
B:HIS155
|
3.2
|
33.7
|
1.0
|
CD2
|
B:HIS214
|
3.2
|
30.2
|
1.0
|
C4
|
B:DHY999
|
3.3
|
43.4
|
1.0
|
CD2
|
B:HIS155
|
3.3
|
33.5
|
1.0
|
OE2
|
B:GLU267
|
3.5
|
35.5
|
1.0
|
NE2
|
B:HIS200
|
3.9
|
33.5
|
1.0
|
OH
|
B:TYR257
|
4.0
|
33.2
|
1.0
|
ND1
|
B:HIS214
|
4.1
|
27.8
|
1.0
|
CG
|
B:HIS214
|
4.3
|
31.1
|
1.0
|
CG
|
B:GLU267
|
4.3
|
31.8
|
1.0
|
ND1
|
B:HIS155
|
4.3
|
32.1
|
1.0
|
C2
|
B:DHY999
|
4.4
|
43.1
|
1.0
|
CB
|
B:GLU267
|
4.4
|
30.4
|
1.0
|
CG
|
B:HIS155
|
4.5
|
33.9
|
1.0
|
ND2
|
B:ASN157
|
4.5
|
38.5
|
1.0
|
CE1
|
B:TYR257
|
4.5
|
31.9
|
1.0
|
CB
|
B:ALA216
|
4.5
|
32.0
|
1.0
|
CE1
|
B:HIS200
|
4.6
|
32.3
|
1.0
|
CZ
|
B:TYR257
|
4.7
|
31.8
|
1.0
|
CB
|
B:ASN157
|
4.7
|
38.5
|
1.0
|
C5
|
B:DHY999
|
4.7
|
40.4
|
1.0
|
NE2
|
B:HIS248
|
4.9
|
37.9
|
1.0
|
CD2
|
B:HIS200
|
5.0
|
36.0
|
1.0
|
|
Iron binding site 3 out
of 4 in 1q0c
Go back to
Iron Binding Sites List in 1q0c
Iron binding site 3 out
of 4 in the Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Fe500
b:34.2
occ:1.00
|
OE1
|
C:GLU267
|
1.9
|
28.3
|
1.0
|
O3
|
C:DHY999
|
2.1
|
44.3
|
1.0
|
NE2
|
C:HIS155
|
2.2
|
22.5
|
1.0
|
NE2
|
C:HIS214
|
2.2
|
25.9
|
1.0
|
O4
|
C:DHY999
|
2.3
|
47.6
|
1.0
|
CE1
|
C:HIS155
|
3.1
|
24.2
|
1.0
|
C3
|
C:DHY999
|
3.1
|
45.5
|
1.0
|
CD
|
C:GLU267
|
3.1
|
28.1
|
1.0
|
CD2
|
C:HIS214
|
3.2
|
27.9
|
1.0
|
CD2
|
C:HIS155
|
3.2
|
18.3
|
1.0
|
CE1
|
C:HIS214
|
3.2
|
29.8
|
1.0
|
C4
|
C:DHY999
|
3.2
|
46.6
|
1.0
|
OE2
|
C:GLU267
|
3.7
|
28.3
|
1.0
|
OH
|
C:TYR257
|
3.9
|
30.0
|
1.0
|
NE2
|
C:HIS200
|
4.0
|
27.3
|
1.0
|
ND2
|
C:ASN157
|
4.2
|
35.9
|
1.0
|
ND1
|
C:HIS155
|
4.2
|
20.4
|
1.0
|
CG
|
C:HIS155
|
4.3
|
22.2
|
1.0
|
ND1
|
C:HIS214
|
4.3
|
27.2
|
1.0
|
CG
|
C:HIS214
|
4.3
|
29.3
|
1.0
|
CG
|
C:GLU267
|
4.4
|
28.3
|
1.0
|
CB
|
C:ALA216
|
4.4
|
27.4
|
1.0
|
C2
|
C:DHY999
|
4.5
|
44.8
|
1.0
|
CE1
|
C:TYR257
|
4.5
|
27.6
|
1.0
|
CB
|
C:GLU267
|
4.5
|
27.8
|
1.0
|
CB
|
C:ASN157
|
4.6
|
31.0
|
1.0
|
C5
|
C:DHY999
|
4.6
|
45.6
|
1.0
|
CE1
|
C:HIS200
|
4.6
|
27.5
|
1.0
|
CZ
|
C:TYR257
|
4.6
|
28.4
|
1.0
|
CG
|
C:ASN157
|
4.9
|
34.8
|
1.0
|
|
Iron binding site 4 out
of 4 in 1q0c
Go back to
Iron Binding Sites List in 1q0c
Iron binding site 4 out
of 4 in the Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate)
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Anerobic Substrate Complex of Homoprotocatechuate 2,3-Dioxygenase From Brevibacterium Fuscum. (Complex with 3,4-Dihydroxyphenylacetate) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Fe500
b:37.6
occ:1.00
|
O3
|
D:DHY999
|
2.0
|
44.7
|
1.0
|
OE1
|
D:GLU267
|
2.0
|
31.1
|
1.0
|
O4
|
D:DHY999
|
2.2
|
46.6
|
1.0
|
NE2
|
D:HIS155
|
2.2
|
24.4
|
1.0
|
NE2
|
D:HIS214
|
2.2
|
27.6
|
1.0
|
C3
|
D:DHY999
|
3.0
|
43.3
|
1.0
|
CD
|
D:GLU267
|
3.0
|
30.3
|
1.0
|
CE1
|
D:HIS155
|
3.0
|
25.4
|
1.0
|
C4
|
D:DHY999
|
3.1
|
44.9
|
1.0
|
CE1
|
D:HIS214
|
3.2
|
28.6
|
1.0
|
CD2
|
D:HIS214
|
3.2
|
26.9
|
1.0
|
CD2
|
D:HIS155
|
3.2
|
23.9
|
1.0
|
OE2
|
D:GLU267
|
3.5
|
33.3
|
1.0
|
OH
|
D:TYR257
|
3.9
|
29.6
|
1.0
|
NE2
|
D:HIS200
|
3.9
|
27.4
|
1.0
|
ND1
|
D:HIS155
|
4.2
|
25.3
|
1.0
|
ND1
|
D:HIS214
|
4.3
|
26.8
|
1.0
|
CG
|
D:GLU267
|
4.3
|
28.8
|
1.0
|
CG
|
D:HIS214
|
4.3
|
28.4
|
1.0
|
CG
|
D:HIS155
|
4.3
|
25.8
|
1.0
|
C2
|
D:DHY999
|
4.3
|
44.0
|
1.0
|
CE1
|
D:TYR257
|
4.4
|
25.3
|
1.0
|
CB
|
D:GLU267
|
4.4
|
27.5
|
1.0
|
ND2
|
D:ASN157
|
4.4
|
32.7
|
1.0
|
C5
|
D:DHY999
|
4.4
|
42.5
|
1.0
|
CB
|
D:ALA216
|
4.5
|
26.5
|
1.0
|
CB
|
D:ASN157
|
4.6
|
31.6
|
1.0
|
CZ
|
D:TYR257
|
4.6
|
27.3
|
1.0
|
CE1
|
D:HIS200
|
4.7
|
25.8
|
1.0
|
CD2
|
D:HIS200
|
5.0
|
23.7
|
1.0
|
CD2
|
D:TYR269
|
5.0
|
42.8
|
1.0
|
|
Reference:
M.W.Vetting,
L.P.Wackett,
L.Que,
J.D.Lipscomb,
D.H.Ohlendorf.
Crystallographic Comparison of Manganese- and Iron-Dependent Homoprotocatechuate 2,3-Dioxygenases. J.Bacteriol. V. 186 1945 2004.
ISSN: ISSN 0021-9193
PubMed: 15028678
DOI: 10.1128/JB.186.7.1945-1958.2004
Page generated: Sat Aug 3 13:14:53 2024
|