Iron in PDB 1uwv: Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
Protein crystallography data
The structure of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase, PDB code: 1uwv
was solved by
T.T.Lee,
S.Agarwalla,
R.M.Stroud,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
50.00 /
1.95
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
35.938,
99.448,
58.519,
90.00,
100.57,
90.00
|
R / Rfree (%)
|
18.6 /
22.7
|
Other elements in 1uwv:
The structure of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
(pdb code 1uwv). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the
Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase, PDB code: 1uwv:
Jump to Iron binding site number:
1;
2;
3;
4;
Iron binding site 1 out
of 4 in 1uwv
Go back to
Iron Binding Sites List in 1uwv
Iron binding site 1 out
of 4 in the Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe1432
b:18.2
occ:1.00
|
FE1
|
A:SF41432
|
0.0
|
18.2
|
1.0
|
S3
|
A:SF41432
|
2.3
|
17.1
|
1.0
|
S4
|
A:SF41432
|
2.3
|
19.0
|
1.0
|
S2
|
A:SF41432
|
2.3
|
19.1
|
1.0
|
SG
|
A:CYS87
|
2.3
|
19.5
|
1.0
|
FE4
|
A:SF41432
|
2.7
|
19.5
|
1.0
|
FE2
|
A:SF41432
|
2.8
|
16.8
|
1.0
|
FE3
|
A:SF41432
|
2.8
|
18.7
|
1.0
|
CB
|
A:CYS87
|
3.1
|
18.9
|
1.0
|
S1
|
A:SF41432
|
3.9
|
16.5
|
1.0
|
CD2
|
A:PHE148
|
4.0
|
14.7
|
1.0
|
CA
|
A:GLY89
|
4.3
|
17.6
|
1.0
|
N
|
A:GLY89
|
4.3
|
17.9
|
1.0
|
N
|
A:CYS90
|
4.5
|
16.6
|
1.0
|
CE2
|
A:PHE148
|
4.5
|
15.7
|
1.0
|
CA
|
A:CYS87
|
4.5
|
18.3
|
1.0
|
C
|
A:GLY89
|
4.7
|
18.3
|
1.0
|
SG
|
A:CYS81
|
4.8
|
20.7
|
1.0
|
SG
|
A:CYS90
|
4.8
|
17.9
|
1.0
|
C
|
A:CYS87
|
4.8
|
17.0
|
1.0
|
SG
|
A:CYS162
|
4.9
|
15.4
|
1.0
|
CB
|
A:HIS83
|
4.9
|
17.8
|
1.0
|
O
|
A:CYS87
|
5.0
|
16.7
|
1.0
|
|
Iron binding site 2 out
of 4 in 1uwv
Go back to
Iron Binding Sites List in 1uwv
Iron binding site 2 out
of 4 in the Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe1432
b:16.8
occ:1.00
|
FE2
|
A:SF41432
|
0.0
|
16.8
|
1.0
|
S3
|
A:SF41432
|
2.3
|
17.1
|
1.0
|
S1
|
A:SF41432
|
2.3
|
16.5
|
1.0
|
S4
|
A:SF41432
|
2.3
|
19.0
|
1.0
|
SG
|
A:CYS162
|
2.3
|
15.4
|
1.0
|
FE4
|
A:SF41432
|
2.6
|
19.5
|
1.0
|
FE1
|
A:SF41432
|
2.8
|
18.2
|
1.0
|
FE3
|
A:SF41432
|
2.8
|
18.7
|
1.0
|
CB
|
A:CYS162
|
3.3
|
15.0
|
1.0
|
CA
|
A:CYS162
|
3.7
|
14.0
|
1.0
|
S2
|
A:SF41432
|
3.9
|
19.1
|
1.0
|
CG1
|
A:ILE164
|
3.9
|
13.0
|
1.0
|
CE2
|
A:PHE148
|
4.0
|
15.7
|
1.0
|
CD1
|
A:ILE164
|
4.2
|
17.2
|
1.0
|
C
|
A:CYS162
|
4.4
|
14.3
|
1.0
|
CD2
|
A:PHE148
|
4.4
|
14.7
|
1.0
|
SG
|
A:CYS81
|
4.5
|
20.7
|
1.0
|
O
|
A:GLN161
|
4.7
|
16.3
|
1.0
|
CD
|
A:PRO163
|
4.8
|
12.5
|
1.0
|
N
|
A:PRO163
|
4.8
|
13.8
|
1.0
|
SG
|
A:CYS90
|
4.9
|
17.9
|
1.0
|
SG
|
A:CYS87
|
4.9
|
19.5
|
1.0
|
N
|
A:CYS162
|
4.9
|
15.2
|
1.0
|
N
|
A:ILE164
|
4.9
|
13.1
|
1.0
|
|
Iron binding site 3 out
of 4 in 1uwv
Go back to
Iron Binding Sites List in 1uwv
Iron binding site 3 out
of 4 in the Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe1432
b:18.7
occ:1.00
|
FE3
|
A:SF41432
|
0.0
|
18.7
|
1.0
|
SG
|
A:CYS90
|
2.3
|
17.9
|
1.0
|
S4
|
A:SF41432
|
2.3
|
19.0
|
1.0
|
S1
|
A:SF41432
|
2.3
|
16.5
|
1.0
|
S2
|
A:SF41432
|
2.3
|
19.1
|
1.0
|
FE4
|
A:SF41432
|
2.7
|
19.5
|
1.0
|
FE2
|
A:SF41432
|
2.8
|
16.8
|
1.0
|
FE1
|
A:SF41432
|
2.8
|
18.2
|
1.0
|
CB
|
A:CYS90
|
3.2
|
16.6
|
1.0
|
NE2
|
A:GLN93
|
3.5
|
19.7
|
1.0
|
N
|
A:CYS90
|
3.8
|
16.6
|
1.0
|
CD1
|
A:ILE164
|
4.0
|
17.2
|
1.0
|
S3
|
A:SF41432
|
4.0
|
17.1
|
1.0
|
CA
|
A:CYS90
|
4.1
|
17.1
|
1.0
|
C
|
A:GLY89
|
4.2
|
18.3
|
1.0
|
CG1
|
A:ILE164
|
4.2
|
13.0
|
1.0
|
CG2
|
A:ILE164
|
4.4
|
14.2
|
1.0
|
CA
|
A:GLY89
|
4.6
|
17.6
|
1.0
|
SG
|
A:CYS162
|
4.8
|
15.4
|
1.0
|
CD
|
A:GLN93
|
4.8
|
17.6
|
1.0
|
SG
|
A:CYS87
|
4.8
|
19.5
|
1.0
|
O
|
A:GLY89
|
4.8
|
18.1
|
1.0
|
SG
|
A:CYS81
|
4.9
|
20.7
|
1.0
|
CB
|
A:CYS81
|
5.0
|
19.7
|
1.0
|
CB
|
A:ILE164
|
5.0
|
13.8
|
1.0
|
|
Iron binding site 4 out
of 4 in 1uwv
Go back to
Iron Binding Sites List in 1uwv
Iron binding site 4 out
of 4 in the Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Crystal Structure of Ruma, the Iron-Sulfur Cluster Containing E. Coli 23S Ribosomal Rna 5-Methyluridine Methyltransferase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe1432
b:19.5
occ:1.00
|
FE4
|
A:SF41432
|
0.0
|
19.5
|
1.0
|
S2
|
A:SF41432
|
2.3
|
19.1
|
1.0
|
S1
|
A:SF41432
|
2.3
|
16.5
|
1.0
|
S3
|
A:SF41432
|
2.3
|
17.1
|
1.0
|
SG
|
A:CYS81
|
2.3
|
20.7
|
1.0
|
FE2
|
A:SF41432
|
2.6
|
16.8
|
1.0
|
FE3
|
A:SF41432
|
2.7
|
18.7
|
1.0
|
FE1
|
A:SF41432
|
2.7
|
18.2
|
1.0
|
CB
|
A:CYS81
|
3.1
|
19.7
|
1.0
|
S4
|
A:SF41432
|
3.8
|
19.0
|
1.0
|
NE2
|
A:GLN93
|
4.0
|
19.7
|
1.0
|
CB
|
A:HIS83
|
4.3
|
17.8
|
1.0
|
CD
|
A:PRO163
|
4.4
|
12.5
|
1.0
|
CA
|
A:CYS162
|
4.5
|
14.0
|
1.0
|
CA
|
A:CYS81
|
4.6
|
18.8
|
1.0
|
O
|
A:GLN161
|
4.6
|
16.3
|
1.0
|
SG
|
A:CYS162
|
4.6
|
15.4
|
1.0
|
N
|
A:PHE84
|
4.6
|
18.4
|
1.0
|
SG
|
A:CYS90
|
4.7
|
17.9
|
1.0
|
SG
|
A:CYS87
|
4.8
|
19.5
|
1.0
|
CB
|
A:CYS87
|
4.9
|
18.9
|
1.0
|
C
|
A:HIS83
|
4.9
|
17.9
|
1.0
|
CB
|
A:CYS162
|
4.9
|
15.0
|
1.0
|
ND1
|
A:HIS83
|
5.0
|
19.6
|
1.0
|
|
Reference:
T.T.Lee,
S.Agarwalla,
R.M.Stroud.
Crystal Structure of Ruma, An Iron-Sulfur Cluster Containing E. Coli Ribosomal Rna 5-Methyluridine Methyltransferase. Structure V. 12 397 2004.
ISSN: ISSN 0969-2126
PubMed: 15016356
DOI: 10.1016/J.STR.2004.02.009
Page generated: Sat Aug 3 16:07:00 2024
|