Iron in PDB 1xm8: X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350

All present enzymatic activity of X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350:
3.1.2.6;

The structure of X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350, PDB code: 1xm8 was solved by G.E.Wesenberg, D.W.Smith, G.N.Phillips Jr., E.Bitto, C.A.Bingman, S.T.M.Allard, Center For Eukaryotic Structural Genomics (Cesg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.71 / 1.74
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	68.494, 58.782, 69.049, 90.00, 109.22, 90.00
R / Rfree (%)	14 / 18.9

The structure of X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350 also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Iron atom in the X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350 (pdb code 1xm8). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350, PDB code: 1xm8:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe701 b:7.6 occ:1.00 NE2 A:HIS59 2.0 10.3 1.0 NE2 A:HIS169 2.0 10.6 1.0 O A:HOH901 2.0 11.7 1.0 OD2 A:ASP131 2.1 9.4 1.0 OD2 A:ASP58 2.1 10.7 1.0 CD2 A:HIS59 2.9 7.3 1.0 CE1 A:HIS169 3.0 12.5 1.0 CG A:ASP131 3.0 11.4 1.0 CD2 A:HIS169 3.0 9.7 1.0 CE1 A:HIS59 3.1 7.5 1.0 CG A:ASP58 3.1 12.6 1.0 OD1 A:ASP131 3.2 8.4 1.0 ZN A:ZN700 3.4 11.1 1.0 OD1 A:ASP58 3.5 13.1 1.0 O A:HOH954 3.6 17.4 1.0 ND2 A:ASN12 4.0 9.6 1.0 CG A:HIS59 4.1 7.7 1.0 NE2 A:HIS54 4.1 10.7 1.0 ND1 A:HIS169 4.1 8.8 1.0 ND1 A:HIS59 4.1 9.2 1.0 CG A:HIS169 4.2 8.9 1.0 CE1 A:HIS54 4.2 9.2 1.0 O B:HOH835 4.3 15.9 1.0 O A:HOH1024 4.3 35.4 1.0 CB A:ASP131 4.4 10.7 1.0 CB A:ASP58 4.5 9.1 1.0 NE2 A:HIS112 4.8 8.2 1.0 OH A:TYR171 5.0 20.8 1.0 CG A:ASN12 5.0 9.9 1.0

Iron binding site 2 out of 2 in the X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of X-Ray Structure of Glyoxalase II From Arabidopsis Thaliana Gene AT2G31350 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe704 b:7.4 occ:1.00 NE2 B:HIS59 2.0 7.0 1.0 O B:HOH802 2.0 11.6 1.0 NE2 B:HIS169 2.0 9.4 1.0 OD2 B:ASP131 2.1 8.8 1.0 OD2 B:ASP58 2.2 9.7 1.0 CD2 B:HIS59 2.9 8.4 1.0 CG B:ASP131 3.0 8.5 1.0 CE1 B:HIS169 3.0 13.2 1.0 CD2 B:HIS169 3.1 10.2 1.0 CE1 B:HIS59 3.1 8.1 1.0 CG B:ASP58 3.2 9.2 1.0 OD1 B:ASP131 3.3 8.6 1.0 ZN B:ZN703 3.3 10.3 1.0 OD1 B:ASP58 3.5 9.8 1.0 O B:HOH843 3.7 16.9 1.0 ND2 B:ASN12 4.0 10.9 1.0 CG B:HIS59 4.1 5.1 1.0 NE2 B:HIS54 4.1 10.2 1.0 ND1 B:HIS169 4.1 10.3 1.0 ND1 B:HIS59 4.2 8.8 1.0 O B:HOH821 4.2 15.7 1.0 CG B:HIS169 4.2 8.6 1.0 CE1 B:HIS54 4.2 11.6 1.0 CB B:ASP131 4.4 9.7 1.0 O B:HOH971 4.4 32.4 1.0 CB B:ASP58 4.6 8.0 1.0 NE2 B:HIS112 4.8 8.2 1.0 O B:HOH1029 4.8 32.3 1.0 CG B:ASN12 5.0 11.3 1.0 OH B:TYR171 5.0 17.6 1.0

G.P.Marasinghe, I.M.Sander, B.Bennett, G.Periyannan, K.W.Yang, C.A.Makaroff, M.W.Crowder. Structural Studies on A Mitochondrial Glyoxalase II. J.Biol.Chem. V. 280 40668 2005.
ISSN: ISSN 0021-9258
PubMed: 16227621
DOI: 10.1074/JBC.M509748200