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Iron in PDB 2b7s: R381K Mutant of Flavocytochrome C3

Enzymatic activity of R381K Mutant of Flavocytochrome C3

All present enzymatic activity of R381K Mutant of Flavocytochrome C3:
1.3.99.1;

Protein crystallography data

The structure of R381K Mutant of Flavocytochrome C3, PDB code: 2b7s was solved by K.L.Pankhurst, C.G.Mowat, E.L.Rothery, C.S.Miles, M.D.Walkinshaw, G.A.Reid, S.K.Chapman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.00 / 2.12
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 45.228, 91.362, 78.193, 90.00, 91.26, 90.00
R / Rfree (%) 14.4 / 19.7

Other elements in 2b7s:

The structure of R381K Mutant of Flavocytochrome C3 also contains other interesting chemical elements:

Sodium (Na) 1 atom

Iron Binding Sites:

The binding sites of Iron atom in the R381K Mutant of Flavocytochrome C3 (pdb code 2b7s). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the R381K Mutant of Flavocytochrome C3, PDB code: 2b7s:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in 2b7s

Go back to Iron Binding Sites List in 2b7s
Iron binding site 1 out of 4 in the R381K Mutant of Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of R381K Mutant of Flavocytochrome C3 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe801

b:14.1
occ:1.00
FE A:HEM801 0.0 14.1 1.0
NE2 A:HIS18 2.0 9.9 1.0
NA A:HEM801 2.0 13.1 1.0
NE2 A:HIS75 2.1 12.0 1.0
NC A:HEM801 2.1 13.8 1.0
NB A:HEM801 2.1 14.1 1.0
ND A:HEM801 2.2 14.1 1.0
CE1 A:HIS18 2.9 9.6 1.0
CD2 A:HIS75 3.0 12.2 1.0
C4A A:HEM801 3.0 12.8 1.0
CD2 A:HIS18 3.0 11.4 1.0
C4C A:HEM801 3.1 14.1 1.0
C1B A:HEM801 3.1 13.5 1.0
C1A A:HEM801 3.1 14.9 1.0
CE1 A:HIS75 3.1 12.3 1.0
C1D A:HEM801 3.1 13.8 1.0
C1C A:HEM801 3.1 15.0 1.0
C4D A:HEM801 3.1 13.2 1.0
C4B A:HEM801 3.2 14.2 1.0
CHD A:HEM801 3.4 12.2 1.0
CHB A:HEM801 3.4 11.5 1.0
CHA A:HEM801 3.5 12.4 1.0
CHC A:HEM801 3.5 13.3 1.0
ND1 A:HIS18 4.1 10.3 1.0
CG A:HIS75 4.1 11.9 1.0
CG A:HIS18 4.2 11.0 1.0
ND1 A:HIS75 4.2 10.4 1.0
C3A A:HEM801 4.3 14.4 1.0
C2A A:HEM801 4.3 12.8 1.0
C3C A:HEM801 4.3 13.0 1.0
C2B A:HEM801 4.3 12.6 1.0
C2C A:HEM801 4.3 14.6 1.0
C3B A:HEM801 4.3 13.4 1.0
C2D A:HEM801 4.4 12.3 1.0
C3D A:HEM801 4.4 13.5 1.0
CB A:ALA74 4.8 12.7 1.0
O A:HOH2023 4.8 21.3 1.0

Iron binding site 2 out of 4 in 2b7s

Go back to Iron Binding Sites List in 2b7s
Iron binding site 2 out of 4 in the R381K Mutant of Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of R381K Mutant of Flavocytochrome C3 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe802

b:14.7
occ:1.00
FE A:HEM802 0.0 14.7 1.0
NC A:HEM802 2.1 15.1 1.0
NB A:HEM802 2.1 13.8 1.0
NA A:HEM802 2.1 14.0 1.0
NE2 A:HIS40 2.1 10.4 1.0
NE2 A:HIS8 2.1 9.9 1.0
ND A:HEM802 2.1 16.0 1.0
CE1 A:HIS40 3.0 10.9 1.0
C4B A:HEM802 3.0 14.0 1.0
C4C A:HEM802 3.1 15.4 1.0
C1C A:HEM802 3.1 13.7 1.0
C1B A:HEM802 3.1 14.9 1.0
CD2 A:HIS8 3.1 11.4 1.0
C1A A:HEM802 3.1 14.5 1.0
CE1 A:HIS8 3.1 12.1 1.0
C4A A:HEM802 3.1 14.2 1.0
C1D A:HEM802 3.1 15.4 1.0
C4D A:HEM802 3.1 13.7 1.0
CD2 A:HIS40 3.1 9.6 1.0
CHC A:HEM802 3.4 13.4 1.0
CHD A:HEM802 3.4 13.7 1.0
CHA A:HEM802 3.4 13.5 1.0
CHB A:HEM802 3.5 12.3 1.0
ND1 A:HIS40 4.1 11.6 1.0
ND1 A:HIS8 4.2 11.8 1.0
CG A:HIS8 4.2 14.1 1.0
CG A:HIS40 4.2 12.9 1.0
C3B A:HEM802 4.3 13.5 1.0
C3C A:HEM802 4.3 15.9 1.0
C2B A:HEM802 4.3 14.8 1.0
C2C A:HEM802 4.3 14.8 1.0
C2A A:HEM802 4.3 12.2 1.0
C3A A:HEM802 4.3 14.3 1.0
C3D A:HEM802 4.3 16.9 1.0
C2D A:HEM802 4.4 14.7 1.0

Iron binding site 3 out of 4 in 2b7s

Go back to Iron Binding Sites List in 2b7s
Iron binding site 3 out of 4 in the R381K Mutant of Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of R381K Mutant of Flavocytochrome C3 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe803

b:11.7
occ:1.00
FE A:HEM803 0.0 11.7 1.0
NB A:HEM803 2.0 11.7 1.0
NA A:HEM803 2.1 12.7 1.0
NE2 A:HIS72 2.1 5.1 1.0
NE2 A:HIS58 2.1 8.4 1.0
NC A:HEM803 2.1 10.7 1.0
ND A:HEM803 2.1 11.7 1.0
C1B A:HEM803 3.0 12.1 1.0
CE1 A:HIS72 3.1 7.0 1.0
C4A A:HEM803 3.1 11.8 1.0
CD2 A:HIS72 3.1 5.7 1.0
CE1 A:HIS58 3.1 7.0 1.0
C4B A:HEM803 3.1 11.3 1.0
C1A A:HEM803 3.1 12.8 1.0
C4D A:HEM803 3.1 13.5 1.0
C1C A:HEM803 3.1 10.3 1.0
CD2 A:HIS58 3.1 7.8 1.0
C4C A:HEM803 3.1 8.7 1.0
C1D A:HEM803 3.2 11.7 1.0
CHB A:HEM803 3.4 12.8 1.0
CHA A:HEM803 3.4 11.3 1.0
CHC A:HEM803 3.5 10.0 1.0
CHD A:HEM803 3.5 7.8 1.0
ND1 A:HIS72 4.2 7.7 1.0
ND1 A:HIS58 4.2 7.0 1.0
CG A:HIS72 4.2 8.5 1.0
C2B A:HEM803 4.2 10.5 1.0
CG A:HIS58 4.3 6.1 1.0
C3B A:HEM803 4.3 10.8 1.0
C3A A:HEM803 4.3 12.6 1.0
C2A A:HEM803 4.3 14.7 1.0
C2C A:HEM803 4.3 9.5 1.0
C3C A:HEM803 4.3 8.8 1.0
C3D A:HEM803 4.3 12.6 1.0
C2D A:HEM803 4.4 10.9 1.0
CG1 A:VAL46 4.9 11.2 1.0

Iron binding site 4 out of 4 in 2b7s

Go back to Iron Binding Sites List in 2b7s
Iron binding site 4 out of 4 in the R381K Mutant of Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of R381K Mutant of Flavocytochrome C3 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe804

b:9.1
occ:1.00
FE A:HEM804 0.0 9.1 1.0
NB A:HEM804 2.0 8.2 1.0
NC A:HEM804 2.1 8.1 1.0
NA A:HEM804 2.1 7.8 1.0
NE2 A:HIS86 2.1 7.8 1.0
ND A:HEM804 2.1 7.6 1.0
NE2 A:HIS61 2.1 5.9 1.0
CD2 A:HIS86 3.0 9.2 1.0
C4B A:HEM804 3.0 9.9 1.0
C1C A:HEM804 3.0 6.9 1.0
C1B A:HEM804 3.1 9.1 1.0
CD2 A:HIS61 3.1 5.6 1.0
C4A A:HEM804 3.1 8.0 1.0
C1A A:HEM804 3.1 9.1 1.0
C4C A:HEM804 3.1 8.8 1.0
C4D A:HEM804 3.1 9.0 1.0
C1D A:HEM804 3.1 8.1 1.0
CE1 A:HIS86 3.1 7.7 1.0
CE1 A:HIS61 3.2 3.4 1.0
CHC A:HEM804 3.4 6.5 1.0
CHB A:HEM804 3.4 7.5 1.0
CHD A:HEM804 3.5 7.7 1.0
CHA A:HEM804 3.5 6.7 1.0
CG A:HIS86 4.2 9.7 1.0
ND1 A:HIS86 4.2 9.4 1.0
CG A:HIS61 4.2 6.8 1.0
ND1 A:HIS61 4.3 6.0 1.0
C2C A:HEM804 4.3 8.3 1.0
C2B A:HEM804 4.3 8.7 1.0
C3B A:HEM804 4.3 8.7 1.0
C3A A:HEM804 4.3 10.0 1.0
C2A A:HEM804 4.3 9.2 1.0
C3C A:HEM804 4.3 9.3 1.0
C3D A:HEM804 4.4 8.7 1.0
C2D A:HEM804 4.4 7.3 1.0

Reference:

K.L.Pankhurst, C.G.Mowat, E.L.Rothery, J.M.Hudson, A.K.Jones, C.S.Miles, M.D.Walkinshaw, F.A.Armstrong, G.A.Reid, S.K.Chapman. A Proton Delivery Pathway in the Soluble Fumarate Reductase From Shewanella Frigidimarina. J.Biol.Chem. V. 281 20589 2006.
ISSN: ISSN 0021-9258
PubMed: 16699170
DOI: 10.1074/JBC.M603077200
Page generated: Sat Aug 3 19:32:49 2024

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