Iron in PDB 2bjj: Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows

The structure of Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows, PDB code: 2bjj was solved by E.A.J.Thomassen, H.A.Van Veen, P.H.C.Van Berkel, J.H.Nuijens, J.P.Abrahams, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.63 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.941, 97.379, 156.297, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 23.1

The binding sites of Iron atom in the Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows (pdb code 2bjj). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows, PDB code: 2bjj:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows within 5.0Å range: probe atom residue distance (Å) B Occ X:Fe693 b:33.7 occ:1.00 OH X:TYR193 1.9 30.3 1.0 OH X:TYR93 1.9 33.8 1.0 O2 X:CO3695 2.2 30.2 1.0 NE2 X:HIS254 2.2 29.7 1.0 OD1 X:ASP61 2.2 32.4 1.0 O3 X:CO3695 2.2 28.5 1.0 C X:CO3695 2.6 29.7 1.0 CZ X:TYR93 3.0 33.2 1.0 CZ X:TYR193 3.1 30.3 1.0 CE1 X:HIS254 3.2 28.3 1.0 CD2 X:HIS254 3.2 30.7 1.0 CG X:ASP61 3.3 30.1 1.0 CE2 X:TYR93 3.6 29.4 1.0 CE1 X:TYR193 3.8 29.8 1.0 O1 X:CO3695 3.8 31.2 1.0 O X:HOH2005 3.9 26.9 1.0 CB X:ASP61 3.9 29.9 1.0 CE1 X:TYR93 3.9 33.2 1.0 CE2 X:TYR193 4.1 30.4 1.0 NH2 X:ARG211 4.2 29.1 1.0 OD2 X:ASP61 4.2 31.4 1.0 CB X:THR123 4.3 31.9 1.0 ND1 X:HIS254 4.3 29.1 1.0 CG X:HIS254 4.3 31.4 1.0 CA X:ASP61 4.5 30.8 1.0 OG1 X:THR123 4.6 30.9 1.0 NH2 X:ARG122 4.7 34.8 1.0 N X:ALA124 4.7 30.2 1.0 N X:THR123 4.9 31.6 1.0 CD2 X:TYR93 4.9 30.5 1.0 NE X:ARG122 5.0 36.6 1.0 N X:GLY62 5.0 30.4 1.0

Iron binding site 2 out of 2 in the Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows within 5.0Å range: probe atom residue distance (Å) B Occ X:Fe694 b:30.1 occ:1.00 OH X:TYR529 1.9 29.2 1.0 OH X:TYR436 1.9 31.9 1.0 O2 X:CO3696 2.1 23.4 1.0 OD1 X:ASP396 2.1 27.6 1.0 O3 X:CO3696 2.3 22.1 1.0 NE2 X:HIS598 2.4 29.8 1.0 C X:CO3696 2.5 22.8 1.0 CZ X:TYR436 3.0 33.6 1.0 CZ X:TYR529 3.0 29.4 1.0 CG X:ASP396 3.2 29.2 1.0 CD2 X:HIS598 3.3 28.5 1.0 CE1 X:HIS598 3.4 25.2 1.0 CE2 X:TYR436 3.5 32.4 1.0 O1 X:CO3696 3.8 23.4 1.0 CE1 X:TYR529 3.8 26.6 1.0 CB X:ASP396 3.8 27.6 1.0 O X:HOH2029 3.9 29.5 1.0 CE2 X:TYR529 3.9 28.1 1.0 CE1 X:TYR436 4.0 31.6 1.0 O X:HOH2036 4.0 29.5 1.0 OD2 X:ASP396 4.2 31.0 1.0 CG X:HIS598 4.5 28.8 1.0 ND1 X:HIS598 4.5 26.9 1.0 CB X:THR467 4.5 31.6 1.0 NH2 X:ARG466 4.5 27.3 1.0 CA X:ASP396 4.5 28.7 1.0 N X:ALA468 4.7 32.8 1.0 OG1 X:THR467 4.8 29.5 1.0 CD2 X:TYR436 4.8 33.3 1.0 N X:THR467 4.9 32.0 1.0 NE X:ARG466 4.9 30.5 1.0

E.A.J.Thomassen, H.A.Van Veen, P.H.C.Van Berkel, J.H.Nuijens, J.P.Abrahams. The Protein Structure of Recombinant Human Lactoferrin Produced in the Milk of Transgenic Cows Closely Matches the Structure of Human Milk-Derived Lactoferrin Transgenic Res. V. 14 397 2005.
ISSN: ISSN 0962-8819
PubMed: 16201406
DOI: 10.1007/S11248-005-3233-0