Atomistry » Iron » PDB 2e1s-2eus » 2e3t
Atomistry »
  Iron »
    PDB 2e1s-2eus »
      2e3t »

Iron in PDB 2e3t: Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)

Enzymatic activity of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)

All present enzymatic activity of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L):
1.17.1.4; 1.17.3.2;

Protein crystallography data

The structure of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L), PDB code: 2e3t was solved by R.Asai, T.Nishino, T.Matsumura, K.Okamoto, E.F.Pai, T.Nishino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.28
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 101.066, 139.513, 222.304, 90.00, 90.00, 90.00
R / Rfree (%) 18.5 / 22.9

Other elements in 2e3t:

The structure of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) also contains other interesting chemical elements:

Calcium (Ca) 4 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) (pdb code 2e3t). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L), PDB code: 2e3t:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Iron binding site 1 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 1 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe2001

b:32.4
occ:1.00
 FE1 A:FES2001 0.0 32.4 1.0
S1 A:FES2001 2.2 34.8 1.0
S2 A:FES2001 2.2 31.5 1.0
SG A:CYS112 2.4 24.4 1.0
SG A:CYS149 2.4 36.7 1.0
FE2 A:FES2001 2.7 32.4 1.0
O A:HOH7011 3.3 17.0 1.0
CB A:CYS112 3.3 24.0 1.0
CB A:CYS149 3.4 30.9 1.0
N A:CYS112 3.7 22.3 1.0
CA A:CYS112 4.0 22.2 1.0
N A:GLY113 4.1 19.1 1.0
N A:CYS149 4.1 26.1 1.0
SG A:CYS147 4.4 27.4 1.0
CA A:CYS149 4.4 29.3 1.0
C A:CYS112 4.4 20.0 1.0
N A:PHE114 4.6 17.4 1.0
N A:ARG148 4.6 27.7 1.0
SG A:CYS115 4.7 30.9 1.0
C A:GLN111 4.8 21.7 1.0
CB A:GLN111 4.9 27.0 1.0
C A:ARG148 4.9 28.1 1.0
N A:CYS115 5.0 19.9 1.0

Iron binding site 2 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 2 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe2001

b:32.4
occ:1.00
 FE2 A:FES2001 0.0 32.4 1.0
S2 A:FES2001 2.2 31.5 1.0
S1 A:FES2001 2.2 34.8 1.0
SG A:CYS115 2.4 30.9 1.0
SG A:CYS147 2.4 27.4 1.0
FE1 A:FES2001 2.7 32.4 1.0
CB A:CYS115 3.4 23.6 1.0
CB A:CYS147 3.4 27.9 1.0
CA A:CYS147 3.8 26.1 1.0
O A:HOH7308 4.2 22.8 1.0
N A:ARG148 4.2 27.7 1.0
N A:CYS115 4.3 19.9 1.0
CA A:CYS115 4.4 20.2 1.0
N A:CYS149 4.4 26.1 1.0
C A:CYS147 4.4 26.5 1.0
SG A:CYS149 4.6 36.7 1.0
CB A:CYS149 4.6 30.9 1.0
SG A:CYS112 4.7 24.4 1.0
CG2 A:THR150 4.7 19.2 1.0
C A:CYS115 5.0 20.8 1.0
N A:GLY113 5.0 19.1 1.0
N A:THR116 5.0 19.7 1.0
N A:THR150 5.0 28.9 1.0

Iron binding site 3 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 3 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe2002

b:21.1
occ:1.00
 FE1 A:FES2002 0.0 21.1 1.0
S2 A:FES2002 2.2 21.3 1.0
S1 A:FES2002 2.2 20.8 1.0
SG A:CYS51 2.4 19.9 1.0
SG A:CYS73 2.4 21.4 1.0
FE2 A:FES2002 2.7 20.8 1.0
CB A:CYS73 3.3 20.2 1.0
CB A:CYS51 3.4 15.8 1.0
N A:GLY44 4.2 22.8 1.0
N A:GLY46 4.2 25.6 1.0
CB A:ASN71 4.2 23.4 1.0
OD1 A:ASN71 4.3 26.6 1.0
N A:CYS73 4.3 18.1 1.0
N A:CYS51 4.3 16.1 1.0
CA A:GLY44 4.4 27.3 1.0
CA A:CYS73 4.4 22.3 1.0
CA A:GLY46 4.4 25.2 1.0
SG A:CYS43 4.5 23.9 1.0
CG A:ASN71 4.5 22.7 1.0
CA A:CYS51 4.5 16.2 1.0
N A:GLU45 4.6 29.3 1.0
SG A:CYS48 4.7 22.2 1.0
N A:GLY49 4.7 19.4 1.0
C A:GLY44 4.8 28.3 1.0
CA A:ASN71 4.9 20.5 1.0

Iron binding site 4 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 4 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe2002

b:20.8
occ:1.00
 FE2 A:FES2002 0.0 20.8 1.0
S2 A:FES2002 2.2 21.3 1.0
S1 A:FES2002 2.2 20.8 1.0
SG A:CYS48 2.4 22.2 1.0
SG A:CYS43 2.4 23.9 1.0
FE1 A:FES2002 2.7 21.1 1.0
N A:CYS43 3.3 21.6 1.0
CB A:CYS48 3.4 17.9 1.0
CB A:CYS43 3.4 20.4 1.0
N A:CYS48 3.5 20.2 1.0
N A:GLY44 3.6 22.8 1.0
N A:GLY49 3.7 19.4 1.0
CA A:CYS43 3.8 23.1 1.0
CA A:CYS48 3.8 19.8 1.0
C A:GLY42 4.1 22.7 1.0
C A:CYS43 4.1 23.8 1.0
C A:CYS48 4.2 18.9 1.0
N A:GLY46 4.3 25.6 1.0
N A:GLY42 4.3 21.2 1.0
N A:GLU45 4.3 29.3 1.0
CA A:GLY42 4.4 20.9 1.0
N A:GLY47 4.4 24.5 1.0
N A:ALA50 4.5 16.3 1.0
SG A:CYS73 4.5 21.4 1.0
CA A:GLY44 4.6 27.3 1.0
C A:GLY47 4.7 25.5 1.0
SG A:CYS51 4.7 19.9 1.0
CA A:GLY49 4.7 19.5 1.0
CA A:GLY46 4.8 25.2 1.0
C A:GLY46 4.9 25.2 1.0
O A:GLY42 5.0 21.7 1.0

Iron binding site 5 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 5 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe2001

b:30.6
occ:1.00
 FE1 B:FES2001 0.0 30.6 1.0
S2 B:FES2001 2.2 26.7 1.0
S1 B:FES2001 2.2 33.9 1.0
SG B:CYS112 2.4 26.0 1.0
SG B:CYS149 2.4 35.6 1.0
FE2 B:FES2001 2.7 30.2 1.0
CB B:CYS112 3.3 26.5 1.0
CB B:CYS149 3.4 30.4 1.0
O B:HOH7015 3.6 23.6 1.0
N B:CYS112 3.7 18.8 1.0
CA B:CYS112 4.0 22.6 1.0
N B:GLY113 4.0 16.3 1.0
N B:CYS149 4.2 24.8 1.0
C B:CYS112 4.4 21.3 1.0
SG B:CYS147 4.4 23.7 1.0
CA B:CYS149 4.4 28.5 1.0
N B:PHE114 4.5 16.1 1.0
SG B:CYS115 4.7 30.3 1.0
N B:ARG148 4.7 24.1 1.0
N B:CYS115 4.9 19.8 1.0
C B:GLN111 4.9 20.1 1.0
CA B:GLY113 5.0 16.8 1.0

Iron binding site 6 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 6 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe2001

b:30.2
occ:1.00
 FE2 B:FES2001 0.0 30.2 1.0
S2 B:FES2001 2.2 26.7 1.0
S1 B:FES2001 2.2 33.9 1.0
SG B:CYS115 2.4 30.3 1.0
SG B:CYS147 2.4 23.7 1.0
FE1 B:FES2001 2.7 30.6 1.0
CB B:CYS115 3.4 22.5 1.0
CB B:CYS147 3.4 24.6 1.0
CA B:CYS147 3.7 22.7 1.0
N B:ARG148 4.2 24.1 1.0
O B:HOH7506 4.2 29.8 1.0
N B:CYS115 4.3 19.8 1.0
CA B:CYS115 4.4 18.6 1.0
N B:CYS149 4.4 24.8 1.0
C B:CYS147 4.4 24.9 1.0
SG B:CYS149 4.5 35.6 1.0
CB B:CYS149 4.5 30.4 1.0
SG B:CYS112 4.7 26.0 1.0
CG2 B:THR150 4.8 23.3 1.0
O B:LEU146 4.9 22.3 1.0
C B:CYS115 5.0 18.4 1.0
N B:THR150 5.0 27.2 1.0

Iron binding site 7 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 7 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe2002

b:20.4
occ:1.00
 FE1 B:FES2002 0.0 20.4 1.0
S1 B:FES2002 2.2 20.3 1.0
S2 B:FES2002 2.2 19.1 1.0
SG B:CYS51 2.4 19.5 1.0
SG B:CYS73 2.4 23.7 1.0
FE2 B:FES2002 2.7 21.2 1.0
CB B:CYS73 3.3 21.1 1.0
CB B:CYS51 3.4 19.7 1.0
OD1 B:ASN71 4.2 21.4 1.0
CB B:ASN71 4.2 18.9 1.0
N B:GLY44 4.2 20.2 1.0
N B:GLY46 4.3 24.2 1.0
N B:CYS51 4.3 18.7 1.0
N B:CYS73 4.3 18.9 1.0
CA B:CYS73 4.4 22.5 1.0
CA B:GLY44 4.5 20.4 1.0
SG B:CYS43 4.5 21.2 1.0
CA B:CYS51 4.5 19.4 1.0
CA B:GLY46 4.5 22.1 1.0
CG B:ASN71 4.5 20.9 1.0
SG B:CYS48 4.7 18.5 1.0
N B:GLY49 4.7 17.6 1.0
N B:GLU45 4.8 23.7 1.0
CA B:ASN71 4.9 19.6 1.0
N B:ALA50 4.9 19.2 1.0
C B:GLY44 4.9 23.3 1.0

Iron binding site 8 out of 8 in 2e3t

Go back to Iron Binding Sites List in 2e3t
Iron binding site 8 out of 8 in the Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of Crystal Structure of Rat Xanthine Oxidoreductase Mutant (W335A and F336L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe2002

b:21.2
occ:1.00
 FE2 B:FES2002 0.0 21.2 1.0
S2 B:FES2002 2.2 19.1 1.0
S1 B:FES2002 2.2 20.3 1.0
SG B:CYS48 2.4 18.5 1.0
SG B:CYS43 2.4 21.2 1.0
FE1 B:FES2002 2.7 20.4 1.0
N B:CYS43 3.4 20.6 1.0
CB B:CYS48 3.4 14.5 1.0
CB B:CYS43 3.5 18.9 1.0
N B:CYS48 3.5 21.5 1.0
N B:GLY44 3.7 20.2 1.0
N B:GLY49 3.8 17.6 1.0
CA B:CYS43 3.8 20.7 1.0
CA B:CYS48 3.9 20.2 1.0
C B:GLY42 4.1 21.5 1.0
C B:CYS48 4.2 20.5 1.0
C B:CYS43 4.2 21.9 1.0
N B:GLY46 4.2 24.2 1.0
N B:GLY42 4.3 23.0 1.0
CA B:GLY42 4.4 19.2 1.0
N B:ALA50 4.4 19.2 1.0
N B:GLY47 4.4 19.9 1.0
N B:GLU45 4.5 23.7 1.0
SG B:CYS73 4.5 23.7 1.0
CA B:GLY44 4.7 20.4 1.0
C B:GLY47 4.7 22.0 1.0
CA B:GLY49 4.7 18.5 1.0
CA B:GLY46 4.7 22.1 1.0
SG B:CYS51 4.8 19.5 1.0
C B:GLY46 4.8 22.5 1.0
O B:GLY42 4.9 20.7 1.0
CB B:ALA50 4.9 14.1 1.0

Reference:

R.Asai, T.Nishino, T.Matsumura, K.Okamoto, K.Igarashi, E.F.Pai, T.Nishino. Two Mutations Convert Mammalian Xanthine Oxidoreductase to Highly Superoxide-Productive Xanthine Oxidase J.Biochem.(Tokyo) V. 141 525 2007.
ISSN: ISSN 0021-924X
PubMed: 17301076
DOI: 10.1093/JB/MVM054
Page generated: Thu Jul 17 00:48:20 2025

Last articles

Fe in 2YXO
Fe in 2YRS
Fe in 2YXC
Fe in 2YNM
Fe in 2YVJ
Fe in 2YP1
Fe in 2YU2
Fe in 2YU1
Fe in 2YQB
Fe in 2YOO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy