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Iron in PDB 2frz: Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)

Enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A):
1.14.15.1;

Protein crystallography data

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A), PDB code: 2frz was solved by Z.Rao, L.L.Wong, F.Xu, S.G.Bell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 66.816, 62.114, 94.940, 90.00, 90.46, 90.00
R / Rfree (%) 19 / 24.3

Other elements in 2frz:

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) also contains other interesting chemical elements:

Potassium (K) 2 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) (pdb code 2frz). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A), PDB code: 2frz:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 2frz

Go back to Iron Binding Sites List in 2frz
Iron binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe417

b:20.2
occ:1.00
FE A:HEM417 0.0 20.2 1.0
NA A:HEM417 2.0 15.1 1.0
NB A:HEM417 2.0 14.6 1.0
NC A:HEM417 2.0 16.7 1.0
ND A:HEM417 2.1 16.3 1.0
SG A:CYS357 2.3 18.2 1.0
O A:HOH1461 2.7 26.3 1.0
C4B A:HEM417 3.0 14.2 1.0
C4A A:HEM417 3.1 14.6 1.0
C1B A:HEM417 3.1 13.9 1.0
C1C A:HEM417 3.1 15.8 1.0
C4D A:HEM417 3.1 15.4 1.0
C1D A:HEM417 3.1 17.8 1.0
C4C A:HEM417 3.1 15.6 1.0
C1A A:HEM417 3.1 14.8 1.0
CB A:CYS357 3.2 14.5 1.0
CHC A:HEM417 3.4 14.3 1.0
CHA A:HEM417 3.5 13.1 1.0
CHD A:HEM417 3.5 18.3 1.0
CHB A:HEM417 3.5 13.9 1.0
CA A:CYS357 3.9 16.5 1.0
C3B A:HEM417 4.3 14.1 1.0
C2B A:HEM417 4.3 13.1 1.0
C3D A:HEM417 4.3 14.6 1.0
C2A A:HEM417 4.3 14.5 1.0
C3A A:HEM417 4.3 13.6 1.0
C2D A:HEM417 4.3 16.6 1.0
C2C A:HEM417 4.3 17.1 1.0
C3C A:HEM417 4.3 16.9 1.0
N A:GLY359 4.4 16.4 1.0
N A:LEU358 4.6 15.9 1.0
C A:CYS357 4.6 17.0 1.0
O A:HOH1570 4.7 23.8 1.0
CA A:GLY359 4.9 15.3 1.0

Iron binding site 2 out of 2 in 2frz

Go back to Iron Binding Sites List in 2frz
Iron binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe417

b:21.5
occ:1.00
FE B:HEM417 0.0 21.5 1.0
NA B:HEM417 2.0 17.1 1.0
NC B:HEM417 2.0 17.2 1.0
NB B:HEM417 2.1 16.1 1.0
ND B:HEM417 2.1 16.7 1.0
SG B:CYS357 2.2 18.9 1.0
O B:HOH2671 2.6 26.4 1.0
C4A B:HEM417 3.0 14.8 1.0
C4B B:HEM417 3.1 16.9 1.0
C4D B:HEM417 3.1 16.3 1.0
C4C B:HEM417 3.1 16.6 1.0
C1D B:HEM417 3.1 16.7 1.0
C1C B:HEM417 3.1 17.1 1.0
C1B B:HEM417 3.1 16.3 1.0
C1A B:HEM417 3.1 16.6 1.0
CB B:CYS357 3.2 17.9 1.0
CHC B:HEM417 3.4 15.8 1.0
CHA B:HEM417 3.5 14.8 1.0
CHD B:HEM417 3.5 16.6 1.0
CHB B:HEM417 3.5 15.4 1.0
CA B:CYS357 3.9 16.6 1.0
C3D B:HEM417 4.3 17.0 1.0
C2A B:HEM417 4.3 16.2 1.0
C3B B:HEM417 4.3 17.0 1.0
C3A B:HEM417 4.3 16.1 1.0
C2B B:HEM417 4.3 16.4 1.0
C2C B:HEM417 4.3 19.1 1.0
C3C B:HEM417 4.3 18.4 1.0
C2D B:HEM417 4.3 16.5 1.0
N B:GLY359 4.5 18.1 1.0
N B:LEU358 4.5 18.9 1.0
C B:CYS357 4.6 17.6 1.0
CA B:GLY359 5.0 18.8 1.0

Reference:

F.Xu, S.G.Bell, G.Taylor, W.Ji, N.Bishop, J.C.Green, Z.Rao, L.L.Wong. Pentachlorobenzene Oxidation By Engineered Cytochrome P450CAM: Substrate Binding and the Mechanism of Aromatic C-H Bond Oxidation To Be Published.
Page generated: Sun Dec 13 14:44:30 2020

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