Iron in PDB 2h7r: L244A Mutant of Cytochrome P450CAM Complexed with Imidazole

All present enzymatic activity of L244A Mutant of Cytochrome P450CAM Complexed with Imidazole:
1.14.15.1;

The structure of L244A Mutant of Cytochrome P450CAM Complexed with Imidazole, PDB code: 2h7r was solved by A.Verras, A.Alian, P.R.Montellano, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.599, 58.784, 57.441, 90.00, 105.29, 90.00
R / Rfree (%)	21 / 28.3

The binding sites of Iron atom in the L244A Mutant of Cytochrome P450CAM Complexed with Imidazole (pdb code 2h7r). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the L244A Mutant of Cytochrome P450CAM Complexed with Imidazole, PDB code: 2h7r:

Iron binding site 1 out of 1 in the L244A Mutant of Cytochrome P450CAM Complexed with Imidazole


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of L244A Mutant of Cytochrome P450CAM Complexed with Imidazole within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe415 b:27.0 occ:1.00 FE A:HEM415 0.0 27.0 1.0 ND A:HEM415 2.0 24.5 1.0 NC A:HEM415 2.1 25.7 1.0 NA A:HEM415 2.1 22.4 1.0 NB A:HEM415 2.1 22.6 1.0 SG A:CYS357 2.4 32.2 1.0 C4 A:1MZ416 2.7 36.3 1.0 N3 A:1MZ416 2.8 37.3 1.0 C1D A:HEM415 3.0 25.2 1.0 C4C A:HEM415 3.0 27.1 1.0 C4D A:HEM415 3.1 24.1 1.0 C1C A:HEM415 3.1 25.4 1.0 C4B A:HEM415 3.1 24.4 1.0 C1A A:HEM415 3.1 23.9 1.0 C4A A:HEM415 3.1 22.2 1.0 C1B A:HEM415 3.1 20.4 1.0 CB A:CYS357 3.3 30.3 1.0 CHD A:HEM415 3.4 26.2 1.0 C5 A:1MZ416 3.4 39.5 1.0 CHC A:HEM415 3.4 25.7 1.0 CHA A:HEM415 3.4 22.1 1.0 C2 A:1MZ416 3.5 40.9 1.0 CHB A:HEM415 3.5 22.8 1.0 N1 A:1MZ416 3.8 39.9 1.0 CA A:CYS357 4.1 31.9 1.0 C2D A:HEM415 4.3 24.4 1.0 C3D A:HEM415 4.3 22.8 1.0 C3C A:HEM415 4.3 27.2 1.0 C2C A:HEM415 4.3 23.8 1.0 C3B A:HEM415 4.3 21.0 1.0 C3A A:HEM415 4.4 22.3 1.0 C2A A:HEM415 4.4 23.4 1.0 C2B A:HEM415 4.4 21.6 1.0 N A:GLY359 4.8 26.8 1.0 C A:CYS357 4.8 30.4 1.0 N A:LEU358 4.8 30.5 1.0

A.Verras, A.Alian, P.R.Montellano. Cytochrome P450 Active Site Plasticity: Attenuation of Imidazole Binding in Cytochrome P450CAM By An L244A Mutation. Protein Eng.Des.Sel. V. 19 491 2006.
ISSN: ISSN 1741-0126
PubMed: 16943206
DOI: 10.1093/PROTEIN/GZL035