Iron in PDB 2h7s: L244A Mutant of Cytochrome P450CAM

All present enzymatic activity of L244A Mutant of Cytochrome P450CAM:
1.14.15.1;

The structure of L244A Mutant of Cytochrome P450CAM, PDB code: 2h7s was solved by A.Verras, A.Alian, P.R.Montellano, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.15
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.704, 59.853, 114.124, 90.00, 104.69, 90.00
R / Rfree (%)	23.5 / 24.9

The binding sites of Iron atom in the L244A Mutant of Cytochrome P450CAM (pdb code 2h7s). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the L244A Mutant of Cytochrome P450CAM, PDB code: 2h7s:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the L244A Mutant of Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of L244A Mutant of Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe415 b:16.2 occ:1.00 FE A:HEM415 0.0 16.2 1.0 NB A:HEM415 1.9 16.9 1.0 NA A:HEM415 2.0 17.2 1.0 NC A:HEM415 2.1 18.4 1.0 ND A:HEM415 2.1 15.3 1.0 SG A:CYS357 2.2 19.1 1.0 O A:HOH518 2.5 24.2 1.0 C1B A:HEM415 2.9 17.1 1.0 C4B A:HEM415 3.0 17.1 1.0 C4A A:HEM415 3.0 15.3 1.0 C4C A:HEM415 3.0 17.4 1.0 C1A A:HEM415 3.0 15.7 1.0 C1D A:HEM415 3.1 15.4 1.0 C4D A:HEM415 3.2 14.7 1.0 C1C A:HEM415 3.2 17.2 1.0 CHD A:HEM415 3.4 16.2 1.0 CB A:CYS357 3.4 18.5 1.0 CHA A:HEM415 3.4 14.3 1.0 CHB A:HEM415 3.5 15.7 1.0 CHC A:HEM415 3.6 16.0 1.0 C2B A:HEM415 4.1 15.5 1.0 C3B A:HEM415 4.2 16.4 1.0 C3A A:HEM415 4.2 15.3 1.0 CA A:CYS357 4.2 17.8 1.0 C2A A:HEM415 4.2 16.3 1.0 C3C A:HEM415 4.3 15.7 1.0 C2D A:HEM415 4.4 14.4 1.0 C2C A:HEM415 4.4 16.1 1.0 C3D A:HEM415 4.4 14.4 1.0 N A:GLY359 4.6 19.0 1.0 CA A:GLY359 4.7 18.8 1.0 C A:CYS357 4.9 19.1 1.0 OG1 A:THR252 4.9 23.9 1.0 N A:LEU358 5.0 20.0 1.0

Iron binding site 2 out of 2 in the L244A Mutant of Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of L244A Mutant of Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe415 b:20.5 occ:1.00 FE C:HEM415 0.0 20.5 1.0 ND C:HEM415 1.8 18.1 1.0 NB C:HEM415 1.9 19.2 1.0 NA C:HEM415 2.0 20.1 1.0 NC C:HEM415 2.0 21.3 1.0 SG C:CYS357 2.1 35.3 1.0 C1D C:HEM415 2.6 19.1 1.0 C4B C:HEM415 2.8 20.2 1.0 C4D C:HEM415 2.9 17.5 1.0 C4A C:HEM415 2.9 18.7 1.0 C4C C:HEM415 2.9 19.7 1.0 C1B C:HEM415 2.9 20.8 1.0 C1C C:HEM415 3.0 20.2 1.0 C1A C:HEM415 3.1 18.3 1.0 O C:HOH416 3.1 5.3 1.0 CHD C:HEM415 3.1 18.5 1.0 CHC C:HEM415 3.3 19.4 1.0 CHB C:HEM415 3.3 19.2 1.0 CHA C:HEM415 3.4 16.8 1.0 CB C:CYS357 3.7 27.9 1.0 C2D C:HEM415 4.0 17.1 1.0 C3A C:HEM415 4.1 18.0 1.0 C3B C:HEM415 4.1 19.5 1.0 C3D C:HEM415 4.1 16.9 1.0 C3C C:HEM415 4.1 18.5 1.0 C2B C:HEM415 4.2 20.0 1.0 C2A C:HEM415 4.2 17.5 1.0 CA C:CYS357 4.3 26.6 1.0 C2C C:HEM415 4.3 19.0 1.0 N C:GLY359 4.3 23.3 1.0 CA C:GLY359 4.5 22.5 1.0 C C:CYS357 4.7 26.8 1.0 N C:LEU358 4.8 26.4 1.0 O C:HOH431 4.9 15.8 1.0

A.Verras, A.Alian, P.R.Montellano. Cytochrome P450 Active Site Plasticity: Attenuation of Imidazole Binding in Cytochrome P450CAM By An L244A Mutation. Protein Eng.Des.Sel. V. 19 491 2006.
ISSN: ISSN 1741-0126
PubMed: 16943206
DOI: 10.1093/PROTEIN/GZL035