Iron in PDB 2iuk: Crystal Structure of Soybean Lipoxygenase-D

All present enzymatic activity of Crystal Structure of Soybean Lipoxygenase-D:
1.13.11.12;

The structure of Crystal Structure of Soybean Lipoxygenase-D, PDB code: 2iuk was solved by B.Youn, G.E.Sellhorn, R.J.Mirchel, B.J.Gaffney, H.D.Grimes, C.Kang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50 / 2.4
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	92.760, 115.100, 120.220, 90.00, 112.34, 90.00
R / Rfree (%)	20.3 / n/a

The binding sites of Iron atom in the Crystal Structure of Soybean Lipoxygenase-D (pdb code 2iuk). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Soybean Lipoxygenase-D, PDB code: 2iuk:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Soybean Lipoxygenase-D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Soybean Lipoxygenase-D within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe901 b:34.0 occ:1.00 OXT A:ILE864 2.0 9.3 1.0 NE2 A:HIS525 2.2 18.0 1.0 NE2 A:HIS530 2.3 21.6 1.0 NE2 A:HIS716 2.3 18.9 1.0 O A:ILE864 2.5 18.7 1.0 C A:ILE864 2.6 19.0 1.0 CE1 A:HIS525 2.9 17.8 1.0 OD1 A:ASN720 3.0 20.9 1.0 CE1 A:HIS530 3.1 9.9 1.0 CD2 A:HIS716 3.3 16.3 1.0 CD2 A:HIS525 3.3 19.4 1.0 CE1 A:HIS716 3.3 25.3 1.0 CD2 A:HIS530 3.4 4.5 1.0 CG A:ASN720 3.7 2.1 1.0 ND1 A:HIS525 4.1 16.4 1.0 CA A:ILE864 4.1 22.6 1.0 CB A:ASN720 4.3 3.8 1.0 ND1 A:HIS530 4.3 6.5 1.0 CG A:HIS525 4.3 13.8 1.0 ND2 A:ASN720 4.4 2.4 1.0 CG A:HIS530 4.5 2.0 1.0 CG A:HIS716 4.5 10.7 1.0 ND1 A:HIS716 4.5 19.5 1.0 N A:ILE864 4.6 17.5 1.0 CG2 A:ILE862 4.7 10.6 1.0 CG2 A:ILE864 4.7 23.5 1.0 CB A:ILE864 5.0 21.8 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Soybean Lipoxygenase-D


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Soybean Lipoxygenase-D within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe1901 b:33.9 occ:1.00 OXT B:ILE1864 2.1 26.9 1.0 NE2 B:HIS1525 2.2 16.1 1.0 NE2 B:HIS1530 2.3 25.9 1.0 NE2 B:HIS1716 2.3 18.6 1.0 O B:ILE1864 2.5 28.4 1.0 C B:ILE1864 2.6 25.3 1.0 CE1 B:HIS1525 2.9 13.2 1.0 OD1 B:ASN1720 3.0 12.7 1.0 CE1 B:HIS1530 3.1 18.3 1.0 CE1 B:HIS1716 3.2 16.3 1.0 CD2 B:HIS1716 3.3 12.1 1.0 CD2 B:HIS1530 3.4 14.9 1.0 CD2 B:HIS1525 3.4 15.3 1.0 CG B:ASN1720 3.6 10.0 1.0 CB B:ASN1720 4.1 13.0 1.0 ND1 B:HIS1525 4.1 15.2 1.0 CA B:ILE1864 4.2 17.1 1.0 ND1 B:HIS1530 4.3 10.7 1.0 CG B:HIS1525 4.4 10.2 1.0 ND1 B:HIS1716 4.4 11.9 1.0 ND2 B:ASN1720 4.4 10.8 1.0 CG B:HIS1530 4.4 14.8 1.0 CG B:HIS1716 4.5 13.7 1.0 N B:ILE1864 4.7 14.5 1.0 CG2 B:ILE1862 4.7 14.0 1.0 CG2 B:ILE1864 4.8 15.7 1.0

B.Youn, G.E.Sellhorn, R.J.Mirchel, B.J.Gaffney, H.D.Grimes, C.Kang. Crystal Structures of Vegetative Soybean Lipoxygenase Vlx-B and Vlx-D, and Comparisons with Seed Isoforms Lox-1 and Lox-3. Proteins V. 65 1008 2006.
ISSN: ISSN 0887-3585
PubMed: 17022084
DOI: 10.1002/PROT.21182