Iron in PDB 2nip: Nitrogenase Iron Protein From Azotobacter Vinelandii

All present enzymatic activity of Nitrogenase Iron Protein From Azotobacter Vinelandii:
1.18.6.1;

The structure of Nitrogenase Iron Protein From Azotobacter Vinelandii, PDB code: 2nip was solved by H.Komiya, M.M.Georgiadis, P.Chakrabarti, D.Woo, J.J.Kornuc, D.C.Rees, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	73.120, 90.530, 91.210, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 29

The binding sites of Iron atom in the Nitrogenase Iron Protein From Azotobacter Vinelandii (pdb code 2nip). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Nitrogenase Iron Protein From Azotobacter Vinelandii, PDB code: 2nip:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe290 b:16.6 occ:1.00 FE1 A:SF4290 0.0 16.6 1.0 S4 A:SF4290 2.1 25.0 1.0 SG B:CYS97 2.1 31.3 1.0 S2 A:SF4290 2.2 22.7 1.0 S3 A:SF4290 2.2 23.0 1.0 FE3 A:SF4290 2.5 19.0 1.0 FE2 A:SF4290 2.5 17.6 1.0 FE4 A:SF4290 2.5 18.8 1.0 CB B:CYS97 3.2 32.7 1.0 CA B:CYS97 3.7 37.3 1.0 S1 A:SF4290 3.7 18.2 1.0 N B:ALA98 4.0 36.0 1.0 C B:CYS97 4.3 37.5 1.0 CA B:GLY134 4.4 18.2 1.0 N B:GLY134 4.5 16.0 1.0 SG A:CYS132 4.5 29.8 1.0 SG B:CYS132 4.5 18.3 1.0 SG A:CYS97 4.5 17.7 1.0 N B:GLY99 4.7 38.1 1.0 N B:CYS97 4.9 39.8 1.0 CB A:CYS97 5.0 17.8 1.0

Iron binding site 2 out of 4 in the Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe290 b:17.6 occ:1.00 FE2 A:SF4290 0.0 17.6 1.0 S1 A:SF4290 2.2 18.2 1.0 S3 A:SF4290 2.2 23.0 1.0 S4 A:SF4290 2.3 25.0 1.0 FE4 A:SF4290 2.3 18.8 1.0 SG A:CYS132 2.3 29.8 1.0 FE3 A:SF4290 2.5 19.0 1.0 FE1 A:SF4290 2.5 16.6 1.0 S2 A:SF4290 3.7 22.7 1.0 CB A:CYS132 3.8 25.7 1.0 N B:ALA98 4.2 36.0 1.0 SG B:CYS97 4.2 31.3 1.0 O A:CYS132 4.3 21.7 1.0 C A:CYS132 4.4 22.4 1.0 SG A:CYS97 4.4 17.7 1.0 CG2 A:VAL130 4.4 17.9 1.0 SG B:CYS132 4.6 18.3 1.0 CB B:ALA98 4.6 32.5 1.0 CA A:CYS132 4.6 23.2 1.0 N A:GLY133 4.8 20.9 1.0 CA B:CYS97 4.8 37.3 1.0 CD2 A:PHE135 4.9 21.7 1.0 CB A:VAL130 4.9 20.8 1.0

Iron binding site 3 out of 4 in the Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe290 b:19.0 occ:1.00 FE3 A:SF4290 0.0 19.0 1.0 SG A:CYS97 2.1 17.7 1.0 S1 A:SF4290 2.1 18.2 1.0 S4 A:SF4290 2.2 25.0 1.0 S2 A:SF4290 2.2 22.7 1.0 FE2 A:SF4290 2.5 17.6 1.0 FE1 A:SF4290 2.5 16.6 1.0 FE4 A:SF4290 2.6 18.8 1.0 CB A:CYS97 3.1 17.8 1.0 CA A:CYS97 3.5 17.6 1.0 S3 A:SF4290 3.8 23.0 1.0 N A:ALA98 4.2 16.4 1.0 C A:CYS97 4.3 19.6 1.0 CA A:GLY133 4.5 20.4 1.0 SG A:CYS132 4.5 29.8 1.0 N A:CYS97 4.6 17.9 1.0 SG B:CYS132 4.6 18.3 1.0 CB B:CYS132 4.6 18.1 1.0 SG B:CYS97 4.6 31.3 1.0 O A:CYS132 4.6 21.7 1.0 N A:GLY133 4.9 20.9 1.0 C A:CYS132 4.9 22.4 1.0 N A:GLY134 4.9 20.0 1.0

Iron binding site 4 out of 4 in the Nitrogenase Iron Protein From Azotobacter Vinelandii


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Nitrogenase Iron Protein From Azotobacter Vinelandii within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe290 b:18.8 occ:1.00 FE4 A:SF4290 0.0 18.8 1.0 S1 A:SF4290 2.1 18.2 1.0 S3 A:SF4290 2.2 23.0 1.0 S2 A:SF4290 2.3 22.7 1.0 FE2 A:SF4290 2.3 17.6 1.0 SG B:CYS132 2.3 18.3 1.0 FE1 A:SF4290 2.5 16.6 1.0 FE3 A:SF4290 2.6 19.0 1.0 CB B:CYS132 3.1 18.1 1.0 S4 A:SF4290 3.6 25.0 1.0 SG B:CYS97 4.3 31.3 1.0 N B:GLY134 4.3 16.0 1.0 SG A:CYS97 4.4 17.7 1.0 CA B:CYS132 4.5 18.6 1.0 CD2 B:PHE135 4.5 25.4 1.0 CA B:GLY134 4.5 18.2 1.0 SG A:CYS132 4.6 29.8 1.0 CE2 B:PHE135 4.6 27.0 1.0 CA A:CYS97 4.7 17.6 1.0 N A:ALA98 4.7 16.4 1.0 C B:CYS132 4.8 22.0 1.0 N B:GLY133 4.9 21.3 1.0

J.L.Schlessman, D.Woo, L.Joshua-Tor, J.B.Howard, D.C.Rees. Conformational Variability in Structures of the Nitrogenase Iron Proteins From Azotobacter Vinelandii and Clostridium Pasteurianum. J.Mol.Biol. V. 280 669 1998.
ISSN: ISSN 0022-2836
PubMed: 9677296
DOI: 10.1006/JMBI.1998.1898