Iron in PDB 2nz5: Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2)

The structure of Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2), PDB code: 2nz5 was solved by B.Zhao, D.C.Lamb, S.L.Kelly, M.R.Waterman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.35
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	192.267, 44.859, 105.052, 90.00, 97.76, 90.00
R / Rfree (%)	23.5 / 29.3

The binding sites of Iron atom in the Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2) (pdb code 2nz5). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2), PDB code: 2nz5:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe430 b:24.1 occ:1.00 FE A:HEM430 0.0 24.1 1.0 ND A:HEM430 2.0 28.1 1.0 NC A:HEM430 2.0 28.6 1.0 NB A:HEM430 2.0 26.3 1.0 NA A:HEM430 2.0 26.7 1.0 SG A:CYS356 2.4 30.9 1.0 OAE A:226431 2.8 61.2 1.0 C1B A:HEM430 3.0 26.8 1.0 C1C A:HEM430 3.0 26.7 1.0 C1A A:HEM430 3.1 26.0 1.0 C4A A:HEM430 3.1 26.6 1.0 C4D A:HEM430 3.1 26.0 1.0 C1D A:HEM430 3.1 25.8 1.0 C4B A:HEM430 3.1 24.3 1.0 C4C A:HEM430 3.1 27.6 1.0 CHB A:HEM430 3.4 27.5 1.0 CHA A:HEM430 3.4 24.6 1.0 CB A:CYS356 3.4 28.9 1.0 CHC A:HEM430 3.4 26.0 1.0 CHD A:HEM430 3.4 25.0 1.0 CAK A:226431 3.5 61.0 1.0 OAA A:226431 4.0 58.1 1.0 CAF A:226431 4.0 60.9 1.0 O A:HOH457 4.1 23.6 1.0 CA A:CYS356 4.2 30.5 1.0 CAO A:226431 4.3 60.0 1.0 C2A A:HEM430 4.3 27.8 1.0 C3A A:HEM430 4.3 28.3 1.0 C2B A:HEM430 4.3 23.9 1.0 C3B A:HEM430 4.3 25.8 1.0 C2D A:HEM430 4.3 25.9 1.0 C2C A:HEM430 4.3 25.0 1.0 C3D A:HEM430 4.3 25.2 1.0 C3C A:HEM430 4.3 28.6 1.0 CAL A:226431 4.5 59.7 1.0 C A:CYS356 4.9 30.2 1.0

Iron binding site 2 out of 2 in the Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure and Function Studies of Cytochrome P450 158A1 From Streptomyces Coelicolor A3(2) within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe430 b:28.9 occ:1.00 FE B:HEM430 0.0 28.9 1.0 ND B:HEM430 2.0 33.2 1.0 NB B:HEM430 2.0 31.1 1.0 NC B:HEM430 2.0 30.1 1.0 NA B:HEM430 2.0 30.9 1.0 SG B:CYS356 2.3 29.4 1.0 OAE B:226431 2.8 57.3 0.5 C4D B:HEM430 3.0 33.9 1.0 C1A B:HEM430 3.1 32.0 1.0 C1B B:HEM430 3.1 29.7 1.0 C1C B:HEM430 3.1 29.5 1.0 C1D B:HEM430 3.1 32.3 1.0 C4B B:HEM430 3.1 28.9 1.0 C4A B:HEM430 3.1 31.2 1.0 C4C B:HEM430 3.1 30.2 1.0 CHA B:HEM430 3.4 32.6 1.0 CHB B:HEM430 3.4 28.8 1.0 CHC B:HEM430 3.4 29.9 1.0 CHD B:HEM430 3.4 32.6 1.0 CB B:CYS356 3.5 30.0 1.0 O B:HOH437 3.8 27.0 1.0 CAK B:226431 3.9 56.6 0.5 CAF B:226431 4.2 56.0 0.5 CA B:CYS356 4.3 30.2 1.0 C3B B:HEM430 4.3 28.7 1.0 C3D B:HEM430 4.3 34.8 1.0 C2B B:HEM430 4.3 29.0 1.0 C2D B:HEM430 4.3 33.9 1.0 C2A B:HEM430 4.3 32.5 1.0 C3A B:HEM430 4.3 30.7 1.0 C2C B:HEM430 4.3 29.6 1.0 C3C B:HEM430 4.3 29.0 1.0 O B:HOH442 4.7 36.3 1.0 OAA B:226431 4.7 55.4 0.5 C B:CYS356 5.0 28.9 1.0 CAO B:226431 5.0 56.5 0.5 N B:GLY358 5.0 24.0 1.0 N B:THR357 5.0 27.2 1.0

B.Zhao, D.C.Lamb, L.Lei, S.L.Kelly, H.Yuan, D.L.Hachey, M.R.Waterman. Different Binding Modes of Two Flaviolin Substrate Molecules in Cytochrome P450 158A1 (CYP158A1) Compared to CYP158A2. Biochemistry V. 46 8725 2007.
ISSN: ISSN 0006-2960
PubMed: 17614370
DOI: 10.1021/BI7006959