Iron in PDB 2p0m: Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase

All present enzymatic activity of Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase:
1.13.11.33;

The structure of Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase, PDB code: 2p0m was solved by J.Choi, J.K.Chon, S.Kim, W.Shin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.99 / 2.40
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	198.900, 198.900, 136.100, 90.00, 90.00, 120.00
R / Rfree (%)	19.3 / 23

The binding sites of Iron atom in the Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase (pdb code 2p0m). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase, PDB code: 2p0m:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe840 b:36.5 occ:1.00 OXT A:ILE663 2.2 49.7 1.0 NE2 A:HIS361 2.2 41.6 1.0 ND1 A:HIS545 2.3 35.8 1.0 NE2 A:HIS541 2.4 24.6 1.0 NE2 A:HIS366 2.4 39.1 1.0 CE1 A:HIS366 3.0 40.7 1.0 CE1 A:HIS361 3.0 44.1 1.0 CE1 A:HIS541 3.0 24.8 1.0 CE1 A:HIS545 3.0 37.2 1.0 C A:ILE663 3.1 46.4 1.0 O A:ILE663 3.2 46.9 1.0 CD2 A:HIS361 3.3 41.6 1.0 CG A:HIS545 3.4 35.8 1.0 CD2 A:HIS541 3.5 26.3 1.0 CD2 A:HIS366 3.7 40.1 1.0 CB A:HIS545 3.9 35.1 1.0 O A:LEU597 4.1 61.3 1.0 ND1 A:HIS361 4.2 42.4 1.0 NE2 A:HIS545 4.2 36.5 1.0 ND1 A:HIS541 4.2 24.3 1.0 ND1 A:HIS366 4.2 39.6 1.0 CG A:HIS361 4.3 40.5 1.0 CD2 A:HIS545 4.4 35.9 1.0 CG A:HIS541 4.5 25.6 1.0 CA A:ILE663 4.6 44.0 1.0 CG A:HIS366 4.6 38.8 1.0 N A:ILE663 4.8 43.6 1.0 NE2 A:GLN548 4.9 39.3 1.0 CG2 A:ILE663 4.9 42.6 1.0

Iron binding site 2 out of 2 in the Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Revised Structure of Rabbit Reticulocyte 15S-Lipoxygenase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe840 b:40.4 occ:1.00 OXT B:ILE663 2.0 43.2 1.0 NE2 B:HIS366 2.1 34.9 1.0 NE2 B:HIS541 2.3 42.7 1.0 ND1 B:HIS545 2.6 36.9 1.0 NE2 B:HIS361 2.6 32.3 1.0 CE1 B:HIS366 2.8 34.4 1.0 C B:ILE663 3.0 40.9 1.0 O B:ILE663 3.1 42.2 1.0 CE1 B:HIS545 3.2 39.1 1.0 CE1 B:HIS541 3.2 42.6 1.0 CD2 B:HIS366 3.3 34.9 1.0 CD2 B:HIS541 3.3 42.7 1.0 CE1 B:HIS361 3.4 32.3 1.0 CD2 B:HIS361 3.5 32.5 1.0 CG B:HIS545 3.8 36.8 1.0 ND1 B:HIS366 4.0 32.3 1.0 CG B:HIS366 4.2 32.9 1.0 CB B:HIS545 4.3 35.8 1.0 C14 B:RS7841 4.3 66.6 1.0 ND1 B:HIS541 4.3 42.0 1.0 CG B:HIS541 4.4 42.5 1.0 CA B:ILE663 4.4 38.6 1.0 NE2 B:HIS545 4.4 39.1 1.0 ND1 B:HIS361 4.5 31.4 1.0 CG1 B:VAL661 4.6 27.8 1.0 CG B:HIS361 4.6 31.8 1.0 CG2 B:ILE663 4.6 39.1 1.0 N B:ILE663 4.7 37.2 1.0 C16 B:RS7841 4.7 66.4 1.0 CD2 B:HIS545 4.7 37.3 1.0

J.Choi, J.K.Chon, S.Kim, W.Shin. Conformational Flexibility in Mammalian 15S-Lipoxygenase: Reinterpretation of the Crystallographic Data. Proteins V. 70 1023 2008.
ISSN: ISSN 0887-3585
PubMed: 17847087
DOI: 10.1002/PROT.21590