Iron in PDB 2qd4: Wild Type Human Ferrochelatase Crystallized with MNCL2

All present enzymatic activity of Wild Type Human Ferrochelatase Crystallized with MNCL2:
4.99.1.1;

The structure of Wild Type Human Ferrochelatase Crystallized with MNCL2, PDB code: 2qd4 was solved by A.E.Medlock, T.A.Dailey, T.A.Ross, H.A.Dailey, W.N.Lanzilotta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.85 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	87.586, 93.062, 109.980, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 23

The structure of Wild Type Human Ferrochelatase Crystallized with MNCL2 also contains other interesting chemical elements:

Chlorine

(Cl)

6 atoms

The binding sites of Iron atom in the Wild Type Human Ferrochelatase Crystallized with MNCL2 (pdb code 2qd4). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Wild Type Human Ferrochelatase Crystallized with MNCL2, PDB code: 2qd4:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Wild Type Human Ferrochelatase Crystallized with MNCL2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Wild Type Human Ferrochelatase Crystallized with MNCL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:20.1 occ:1.00 FE1 A:FES501 0.0 20.1 1.0 S2 A:FES501 2.2 20.3 1.0 S1 A:FES501 2.2 19.9 1.0 SG A:CYS411 2.3 21.3 1.0 SG A:CYS406 2.3 20.9 1.0 FE2 A:FES501 2.7 19.2 1.0 CB A:CYS411 3.2 22.6 1.0 CB A:CYS406 3.4 21.8 1.0 O A:HOH959 4.2 41.4 1.0 CA A:CYS406 4.2 22.1 1.0 O A:HOH826 4.2 19.1 1.0 SG A:CYS196 4.5 20.0 1.0 O A:HOH961 4.5 31.7 1.0 CB A:ASN408 4.6 24.9 1.0 SG A:CYS403 4.6 20.1 1.0 O A:ASN408 4.6 24.9 1.0 CA A:CYS411 4.6 23.6 1.0 N A:CYS403 4.7 20.7 1.0 CB A:CYS403 4.7 20.4 1.0 CB A:SER402 4.8 21.7 1.0 O A:HOH960 4.9 32.8 1.0 CB A:CYS196 4.9 18.8 1.0 N A:ASN408 5.0 24.9 1.0

Iron binding site 2 out of 4 in the Wild Type Human Ferrochelatase Crystallized with MNCL2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Wild Type Human Ferrochelatase Crystallized with MNCL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:19.2 occ:1.00 FE2 A:FES501 0.0 19.2 1.0 S1 A:FES501 2.2 19.9 1.0 S2 A:FES501 2.3 20.3 1.0 SG A:CYS196 2.3 20.0 1.0 SG A:CYS403 2.3 20.1 1.0 FE1 A:FES501 2.7 20.1 1.0 CB A:CYS403 3.4 20.4 1.0 CB A:CYS196 3.4 18.8 1.0 O A:HOH889 3.7 24.3 1.0 N A:CYS403 3.8 20.7 1.0 CA A:CYS403 4.2 20.7 1.0 O A:HOH958 4.3 25.0 1.0 SG A:CYS411 4.5 21.3 1.0 SG A:CYS406 4.5 20.9 1.0 O A:HOH959 4.6 41.4 1.0 CB A:CYS406 4.6 21.8 1.0 CD A:ARG272 4.7 25.3 1.0 CA A:CYS196 4.7 18.1 1.0 NH1 A:ARG272 4.9 27.2 1.0 NH2 B:ARG798 4.9 15.5 1.0 C A:SER402 5.0 20.9 1.0

Iron binding site 3 out of 4 in the Wild Type Human Ferrochelatase Crystallized with MNCL2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Wild Type Human Ferrochelatase Crystallized with MNCL2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe502 b:20.5 occ:1.00 FE1 B:FES502 0.0 20.5 1.0 S2 B:FES502 2.2 21.1 1.0 S1 B:FES502 2.2 20.6 1.0 SG B:CYS911 2.3 22.3 1.0 SG B:CYS906 2.3 21.8 1.0 FE2 B:FES502 2.7 20.1 1.0 CB B:CYS911 3.2 23.4 1.0 CB B:CYS906 3.4 22.6 1.0 CA B:CYS906 4.2 23.0 1.0 O B:HOH953 4.3 22.5 1.0 O B:HOH1101 4.4 28.7 1.0 O B:HOH1051 4.4 58.5 1.0 CB B:ASN908 4.5 25.4 1.0 SG B:CYS696 4.5 21.6 1.0 SG B:CYS903 4.5 20.4 1.0 O B:ASN908 4.6 25.4 1.0 CA B:CYS911 4.6 24.4 1.0 CB B:CYS903 4.7 20.8 1.0 N B:CYS903 4.7 20.9 1.0 N B:ASN908 4.9 25.2 1.0 CB B:SER902 4.9 21.7 1.0 CB B:CYS696 4.9 20.6 1.0

Iron binding site 4 out of 4 in the Wild Type Human Ferrochelatase Crystallized with MNCL2


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Wild Type Human Ferrochelatase Crystallized with MNCL2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe502 b:20.1 occ:1.00 FE2 B:FES502 0.0 20.1 1.0 S1 B:FES502 2.2 20.6 1.0 S2 B:FES502 2.3 21.1 1.0 SG B:CYS696 2.3 21.6 1.0 SG B:CYS903 2.3 20.4 1.0 FE1 B:FES502 2.7 20.5 1.0 CB B:CYS903 3.4 20.8 1.0 CB B:CYS696 3.4 20.6 1.0 O B:HOH1052 3.7 27.6 1.0 N B:CYS903 3.9 20.9 1.0 CA B:CYS903 4.2 21.0 1.0 O B:HOH1050 4.2 25.8 1.0 O B:HOH1051 4.3 58.5 1.0 SG B:CYS911 4.5 22.3 1.0 SG B:CYS906 4.5 21.8 1.0 CD B:ARG772 4.5 25.0 1.0 CB B:CYS906 4.6 22.6 1.0 CA B:CYS696 4.7 20.3 1.0 NH1 B:ARG772 4.9 26.0 1.0 NH2 A:ARG298 4.9 15.8 1.0 C B:SER902 5.0 21.0 1.0

A.E.Medlock, T.A.Dailey, T.A.Ross, H.A.Dailey, W.N.Lanzilotta. A Pi-Helix Switch Selective For Porphyrin Deprotonation and Product Release in Human Ferrochelatase. J.Mol.Biol. V. 373 1006 2007.
ISSN: ISSN 0022-2836
PubMed: 17884090
DOI: 10.1016/J.JMB.2007.08.040